Publications

Complete List of Published Work in MyBibliography:

https://www.ncbi.nlm.nih.gov/myncbi/carlos.subauste.1/bibliography/public/

Selected Publications

1.    Vos S, Portillo JC, Hubal A, Bapputty R, Pfaff A, Aaron R, Weng M, Sun D, Lu Z-R, Yu J-S, Subauste CS. CD40 Induces Unfolded Protein Response, Upregulation of VEGF, and Vascular Leakage in Diabetic Retinopathy. Diabetes 2025. Feb 20.

2.   Hubal A, Vendhoti A, Shaffer CN, Vos S, Lopez Corcino Y, Subauste CS. 2025. Inhibition of Src signaling induces autophagic killing of Toxoplasma gondii via PTEN-mediated deactivation of Akt. PLoS Pathogens Jan 27, 2025 https://doi.org/10.1371/journal.ppat.1012907

3. Hubal A, Pfaff A, Vos S, Upadhyay M, Bonilha V, Subauste CS. A Simplified Method for Isolation and Culture of Retinal Pigment Epithelial Cells from Adult Mice. J Vis Exp. 2024 May 24;(207). doi: 10.3791/66921.

4. Portillo JC, Pfaff A, Vos S, Weng M, Nagaraj RH, Subauste CS. Advanced Glycation End Products Upregulate CD40 in Human Retinal Endothelial and Müller Cells: Relevance to Diabetic Retinopathy. Cells. 2024 Feb 29;13(5):429. doi: 10.3390/cells13050429.PMID: 38474393 

5. Vos S, Aaron R, Weng M, Daw J, Rodriguez-Rivera E, Subauste CS. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression. Invest Ophthalmol Vis Sci. 2023 Jun 1;64(7):17. doi: 10.1167/iovs.64.7.17.PMID: 37294707 

6. Portillo JC, Yu JS, Vos S, Bapputty R, Lopez Corcino Y, Hubal A, Daw J, Arora S, Sun W, Lu ZR, Subauste CS. Disruption of retinal inflammation and the development of diabetic retinopathy in mice by a CD40-derived peptide or mutation of CD40 in Müller cells. Diabetologia. 2022 Dec;65(12):2157-2171. doi: 10.1007/s00125-022-05775-6. Epub 2022 Aug 3.PMID: 35920844 

7. Yu JS, Daw J, Portillo JC, Subauste CS. CD40 Expressed in Endothelial Cells Promotes Upregulation of ICAM-1 But Not Pro-Inflammatory Cytokines, NOS2 and P2X7 in the Diabetic Retina. Invest Ophthalmol Vis Sci. 2021 Sep 2;62(12):22. doi: 10.1167/iovs.62.12.22.PMID: 34546322 

8. Portillo JC, Yu JS, Hansen S, Kern TS, Subauste MC, Subauste CS. A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion. FASEB J. 2021 Mar;35(3):e21412. doi: 10.1096/fj.201903203RR.PMID: 33675257

9. Subauste CS. The CD40-ATP-P2X 7 Receptor Pathway: Cell to Cell Cross-Talk to Promote Inflammation and Programmed Cell Death of Endothelial Cells. Front Immunol. 2019 Dec 17;10:2958. doi: 10.3389/fimmu.2019.02958. eCollection 2019.PMID: 31921199 

10. Lopez Corcino Y, Gonzalez Ferrer S, Mantilla LE, Trikeriotis S, Yu JS, Kim S, Hansen S, Portillo JC, Subauste CS. Toxoplasma gondii induces prolonged host epidermal growth factor receptor signalling to prevent parasite elimination by autophagy: Perspectives for in vivo control of the parasite. Cell Microbiol. 2019 Oct;21(10):e13084. doi: 10.1111/cmi.13084. Epub 2019 Jul 17.PMID: 31290228 

11. Portillo JC, Van Grol J, Saffo S, Lopez Corcino Y, Rodriguez M, Fox BA, Bzik DJ, Ward NL, Dubyak GR, Rojas RE, Toosi Z, Subauste CS. CD40 in Endothelial Cells Restricts Neural Tissue Invasion by Toxoplasma gondii. Infect Immun. 2019 Jul 23;87(8):e00868-18. doi: 10.1128/IAI.00868-18. Print 2019 Aug.PMID: 31109947

11. Corcino YL, Portillo JC, Subauste CS. Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii. Sci Rep. 2019 Jan 24;9(1):669. doi: 10.1038/s41598-018-36724-2.PMID: 30679495 

13. Portillo JC, Muniz-Feliciano L, Lopez Corcino Y, Lee SJ, Van Grol J, Parsons SJ, Schiemman WP, Subauste CS. Toxoplasma gondii induces FAK-Src-STAT3 signaling during infection of host cells that prevents parasite targeting by autophagy. PLoS Pathog. 2017 Oct 16;13(10):e1006671. doi: 10.1371/journal.ppat.1006671. eCollection 2017 Oct.PMID: 29036202

14. Portillo JC, Lopez Corcino Y, Dubyak GR, Kern TS, Matsuyama S, Subauste CS. Ligation of CD40 in Human Müller Cells Induces P2X7 Receptor-Dependent Death of Retinal Endothelial Cells. Invest Ophthalmol Vis Sci. 2016 Nov 1;57(14):6278-6286. doi: 10.1167/iovs.16-20301.PMID: 27893093

15. Portillo JC, Lopez Corcino Y, Miao Y, Tang J, Sheibani N, Kern TS, Dubyak GR, Subauste CS. CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes. 2017 Feb;66(2):483-493. doi: 10.2337/db16-0051. Epub 2016 Jul 29.PMID: 27474370

16. Liu E, Lopez Corcino Y, Portillo JA, Miao Y, Subauste CS. Identification of Signaling Pathways by Which CD40 Stimulates Autophagy and Antimicrobial Activity against Toxoplasma gondii in Macrophages. Infect Immun. 2016 Aug 19;84(9):2616-26. doi: 10.1128/IAI.00101-16. Print 2016 Sep.PMID: 27354443

17. Portillo JA, Schwartz I, Zarini S, Bapputty R, Kern TS, Gubitosi-Klug RA, Murphy RC, Subauste MC, Subauste CS. Proinflammatory responses induced by CD40 in retinal endothelial and Müller cells are inhibited by blocking CD40-Traf2,3 or CD40-Traf6 signaling. Invest Ophthalmol Vis Sci. 2014 Dec 4;55(12):8590-7. doi: 10.1167/iovs.14-15340.PMID: 25477319

18. Portillo JA, Greene JA, Schwartz I, Subauste MC, Subauste CS. Blockade of CD40-TRAF2,3 or CD40-TRAF6 is sufficient to inhibit pro-inflammatory responses in non-haematopoietic cells. Immunology. 2015 Jan;144(1):21-33. doi: 10.1111/imm.12361.PMID: 25051892

19. Portillo JA, Greene JA, Okenka G, Miao Y, Sheibani N, Kern TS, Subauste CS. CD40 promotes the development of early diabetic retinopathy in mice. Diabetologia. 2014 Oct;57(10):2222-31. doi: 10.1007/s00125-014-3321-x.PMID: 25015056

20. Muniz-Feliciano L, Van Grol J, Portillo JA, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. Toxoplasma gondii-induced activation of EGFR prevents autophagy protein-mediated killing of the parasite. PLoS Pathog. 2013;9(12):e1003809. Epub 2013 Dec 19.PMID: 24367261

21. Ogolla PS, Portillo JA, White CL, Patel K, Lamb B, Sen GC, Subauste CS. The protein kinase double-stranded RNA-dependent (PKR) enhances protection against disease cause by a non-viral pathogen. PLoS Pathog. 2013;9(8):e1003557. doi: 10.1371/journal.ppat.1003557. Epub 2013 Aug 22.PMID: 23990781

22. Van Grol J, Muniz-Feliciano L, Portillo JA, Bonilha VL, Subauste CS. CD40 induces anti-Toxoplasma gondii activity in nonhematopoietic cells dependent on autophagy proteins. Infect Immun. 2013 Jun;81(6):2002-11. doi: 10.1128/IAI.01145-12. Epub 2013 Mar 18.PMID: 23509150 

23. Portillo JA, Okenka G, Reed E, Subauste A, Van Grol J, Gentil K, Komatsu M, Tanaka K, Landreth G, Levine B, Subauste CS. The CD40-autophagy pathway is needed for host protection despite IFN-Γ-dependent immunity and CD40 induces autophagy via control of P21 levels. PLoS One. 2010 Dec 31;5(12):e14472. doi: 10.1371/journal.pone.0014472.PMID: 21217818

24. Van Grol J, Subauste C, Andrade RM, Fujinaga K, Nelson J, Subauste CS. HIV-1 inhibits autophagy in bystander macrophage/monocytic cells through Src-Akt and STAT3. PLoS One. 2010 Jul 22;5(7):e11733. PMID: 20661303 

25. Portillo JA, Van Grol J, Zheng L, Okenka G, Gentil K, Garland A, Carlson EC, Kern TS, Subauste CS. CD40 mediates retinal inflammation and neurovascular degeneration. J Immunol. 2008 Dec 15;181(12):8719-26. doi: 10.4049/jimmunol.181.12.8719.PMID: 19050292

26. Subauste CS, Andrade RM, Wessendarp M. CD40-TRAF6 and autophagy-dependent anti-microbial activity in macrophages. Autophagy. 2007 May-Jun;3(3):245-8. doi: 10.4161/auto.3717. Epub 2007 May 15.PMID: 17224624

27. Andrade RM, Wessendarp M, Gubbels MJ, Striepen B, Subauste CS. CD40 induces macrophage anti-Toxoplasma gondii activity by triggering autophagy-dependent fusion of pathogen-containing vacuoles and lysosomes. J Clin Invest. 2006 Sep;116(9):2366-77. doi: 10.1172/JCI28796.PMID: 16955139

28. Subauste CS, Wessendarp M, Sorensen RU, Leiva LE. CD40-CD40 ligand interaction is central to cell-mediated immunity against Toxoplasma gondii: patients with hyper IgM syndrome have a defective type 1 immune response that can be restored by soluble CD40 ligand trimer. J Immunol. 1999 Jun 1;162(11):6690-700.PMID: 10352287