Photomedicine and Biophysical Microscopy of Cancer

Image Credit: Bryan Spring

Image Credit: Nima Davoudzadeh

The Spring Laboratory at Northeastern University bridges biophysics, biomedical optics and cancer biology to selectively target microscopic deposits of tumor cells left behind by standard therapies that limit our ability to cure many malignancies. Optical spectroscopic imaging and photophysics are applied to visualize and mop up this residual disease. The ultimate goal of the program is to reduce cancer recurrence and mortality by establishing new approaches for personalized medicine that address tumor heterogeneity, drug-resistance and molecular mechanisms of treatment escape.

Advanced-stage cancer patients are presently subjected to a grueling treatment regimen consisting of surgical tumor debulking and high-dose-intensity chemotherapy. These standard approaches frequently hit a wall due to dose-limiting toxicities as well as mechanisms of drug-resistance and treatment escape via cell signaling networks. To address these challenges, the group’s efforts focus on developments in photomedicine in concert with guidance from in vivo microscopy for the discovery of dynamic molecular mechanisms of treatment escape and for the rational design of therapeutic regimens that overcome resistance. In vivo imaging is used for microscopic-resolution “optical biopsy” to identify drug-resistant cancer cells and to monitor cell signaling events without the need for invasive surgeries and tissue biopsies that can miss smaller lesions. Near infrared light activation of molecular-targeted chromophores is applied to selectively damage drug-resistant cancer cells, to suppress mechanisms of treatment escape and to sensitize the tumor to systemic therapies—including chemotherapy and immunotherapy.

A major focus of the group is to develop multicolor microendoscopy and molecular-targeted agents for both imaging and therapy. The group builds new fluorescence microscopy tools (e.g., hyperspectral, lifetime-resolved and FRET imaging); develops real-time image computation software; designs and synthesizes molecular-targeted probes; and, develops mouse models of cancer when required.

Latest Publication

Davoudzadeh N, Ducourthial G, Spring BQ. "Custom fabrication and mode-locked operation of a femtosecond fiber laser for multiphoton microscopy". Sci Rep 2019; 9: 4233. Download PDF

News

June 28 to July 4, 2019 - The Spring Research Group presented at the International Photodynamic Association (IPA) World Congress 2019 in Boston. Congratulations to Eric, Arvind, and Ryan and all the poster award winners:

Eric Kercher — Poster of Excellence (Top 10) and Invited Slam Talk

Arvind Mohan — Poster of Excellence (Top 10) and Invited Slam Talk

Ryan Lang — Poster of Merit (Top 20)

IPA Gala: View from the State roomIPA Gala dinnerIPA Gala: Awards ceremony with fireworks over the harborEric presenting his posterCarousel imageCarousel image

June 6, 2019 - Congratulations to Eric Kercher who passed his preliminary thesis examination!

Photo: group dinner at Petit Robert Bistro to celebrate.


Feb 1 to 5, 2019 - The Spring Research Group presented at Photonics West 2019 in San Franciscio.

Eric Kercher: Real-time GPU accelerated hyperspectral unmixing, Poster 10889-40

Ryan Lang: Micro-image mosaicking for video-rate multi-channel fluorescence microendoscopy, Contributed oral presentation 10854-40, Endoscopic Microscopy

Kai Zhang: Custom fabrication of a high-performance femtosecond fiber laser for multiphoton microscopy, Contributed oral presentation 10882-28, Multiphoton Microscopy in the Biomedical Sciences

Bryan Spring: Targeting drug-resistant glioblastoma stem cells using photodynamic therapy, Invited oral presentation 10860-3, Photodynamic Therapy


Sept 6, 2018 - Members of the Spring Lab present their work at the annual Smith Family Foundation Scientific Poster Session.


The Smith Family Awards Program for Excellence in Biomedical Research has awarded $32.8 million in grants to support 163 newly independent faculty over the past 26 years. Each investigator receives $300,000 over three years.


Professor Spring received this prestigious award in 2017 for his project entitled: "Peering into Cancer Stem Cell Niches to Guide Suppression of Multiple Signaling Loop Pathways"

May 15, 2018 - Congrats to Ryan, Kai, Arvind, and Dr. Spring for their great presentations at the 2018 American Society for Photobiology Conference in Tampa Bay!

Visit to Moffitt Cancer Center to explore a new collaboration among mathematical oncologists, biomedical physicists and ovarian tumor immunologists: Bob Gatenby, Heiko Enderling, Jose Conejo-Garcia, and Bryan Spring (May 15, 2018; Tampa).