Photomedicine and Biophysical Microscopy of Cancer
Image Credit: Bryan Spring
Image Credit: Nima Davoudzadeh
The Spring Laboratory at Northeastern University bridges biophysics, biomedical optics and cancer biology to selectively target microscopic deposits of tumor cells left behind by standard therapies that limit our ability to cure many malignancies. Optical spectroscopic imaging and photophysics are applied to visualize and mop up this residual disease. The ultimate goal of the program is to reduce cancer recurrence and mortality by establishing new approaches for personalized medicine that address tumor heterogeneity, drug-resistance and molecular mechanisms of treatment escape.
Advanced-stage cancer patients are presently subjected to a grueling treatment regimen consisting of surgical tumor debulking and high-dose-intensity chemotherapy. These standard approaches frequently hit a wall due to dose-limiting toxicities as well as mechanisms of drug-resistance and treatment escape via cell signaling networks. To address these challenges, the group’s efforts focus on developments in photomedicine in concert with guidance from in vivo microscopy for the discovery of dynamic molecular mechanisms of treatment escape and for the rational design of therapeutic regimens that overcome resistance. In vivo imaging is used for microscopic-resolution “optical biopsy” to identify drug-resistant cancer cells and to monitor cell signaling events without the need for invasive surgeries and tissue biopsies that can miss smaller lesions. Near infrared light activation of molecular-targeted chromophores is applied to selectively damage drug-resistant cancer cells, to suppress mechanisms of treatment escape and to sensitize the tumor to systemic therapies—including chemotherapy and immunotherapy.
A major focus of the group is to develop multicolor microendoscopy and molecular-targeted agents for both imaging and therapy. The group builds new fluorescence microscopy tools (e.g., hyperspectral, lifetime-resolved and FRET imaging); develops real-time image computation software; designs and synthesizes molecular-targeted probes; and, develops mouse models of cancer when required.
Lang RT, Tatz J, Kercher EM, Palanisami A, Brooks DH, Spring BQ. "Multi-channel correlation improves the noise tolerance of real-time hyperspectral micro-image mosaicking". J Biomed Opt, 24(12), 126002 (2019). Download PDF
May 1, 2020 - Congratulations to Dr. Eric Kercher who successfully defended his doctoral dissertation "New Methods and Tools for Optimizing Precision Photomedicine Research." Eric is the Spring Lab's inaugural PhD student and graduate. Eric's publication record speaks for itself—he has co-authored 8 papers published and under review during his graduate training spanning photomedicine, cancer biology and new device development.
April 1, 2020 - The Spring Laboratory has been awarded a NIH-NCI R01 award (R01 CA226855, $3.2M) titled "Multiplexed and dynamically targeted photoimmunotherapy of heterogeneous, chemoresistant micrometastases guided by online in vivo optical imaging of cell-surface biomarkers".
June 28 to July 4, 2019 - The Spring Research Group presented at the International Photodynamic Association (IPA) World Congress 2019 in Boston. Congratulations to Eric, Arvind, and Ryan and all the poster award winners:
Eric Kercher — Poster of Excellence (Top 10) and Invited Slam Talk
Arvind Mohan — Poster of Excellence (Top 10) and Invited Slam Talk
Ryan Lang — Poster of Merit (Top 20)
June 6, 2019 - Congratulations to Eric Kercher who passed his preliminary thesis examination!
Photo: group dinner at Petit Robert Bistro to celebrate. Left to right: Liam Price, Becca Harman, Alejandro Olmos, Bryan Spring, Ryan Lang, and Eric Kercher.
Feb 1 to 5, 2019 - The Spring Research Group presented at Photonics West 2019 in San Franciscio.
Eric Kercher: Real-time GPU accelerated hyperspectral unmixing, Poster 10889-40
Ryan Lang: Micro-image mosaicking for video-rate multi-channel fluorescence microendoscopy, Contributed oral presentation 10854-40, Endoscopic Microscopy
Kai Zhang: Custom fabrication of a high-performance femtosecond fiber laser for multiphoton microscopy, Contributed oral presentation 10882-28, Multiphoton Microscopy in the Biomedical Sciences
Bryan Spring: Targeting drug-resistant glioblastoma stem cells using photodynamic therapy, Invited oral presentation 10860-3, Photodynamic Therapy
Sept 6, 2018 - Members of the Spring Lab present their work at the annual Smith Family Foundation Scientific Poster Session.
The Smith Family Awards Program for Excellence in Biomedical Research has awarded $32.8 million in grants to support 163 newly independent faculty over the past 26 years. Each investigator receives $300,000 over three years.
Professor Spring received this prestigious award in 2017 for his project entitled: "Peering into Cancer Stem Cell Niches to Guide Suppression of Multiple Signaling Loop Pathways"
Left to right: Kai Zhang, Eric Kercher, Ryan Lang, and Taresh Sharan.
Visit to Moffitt Cancer Center to explore a new collaboration among mathematical oncologists, biomedical physicists and ovarian tumor immunologists. Left to right: Jose Conejo-Garcia, Bryan Spring, Heiko Enderling, and Bob Gatenby (May 15, 2018; Tampa).