Resume

Current Academic Affiliation

Banaras Hindu University (B.H.U.) (2017-19): M.Sc. Applied Microbiology
Gargi College, University of Delhi (2014-17): B.Sc. (H) Microbiology
Udai Pratap School, Varanasi, Uttar Pradesh (2014): Senior Secondary School Certificate (Science)
Udai Pratap School, Varanasi, Uttar Pradesh (2014): Higher Secondary School Certificate

Dissertation Project under Dr. Rajan Sankarnarayanan in Centre for Cellular and Molecular Biology (CCMB), Hyderabad (January-June 2019)
IAS-INSA-NASI Summer Research Fellow (May-July 2018): Undertook a project entitled ‘Characterisation of Chaperonin Proteins in Candida glabrata’ as IAS-INSA-NASI Summer Research Fellow under Dr. Rupinder Kaur, Laboratory of Fungal Pathogenesis, Centre for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad.

Microbiological techniques: Generation of knockouts by homologous recombination in Candida, Knockout screening in Candida, Gene deletion strategy, Bacterial and yeast transformation.
Bacterial cell culture: E. coli- (DH-5α and Rosetta), Making competent and ultra-competent E. coli cells, Blue-white screening, Spotting assay, Replica plating.
Molecular techniques: Molecular Cloning [Restriction-free (RF) cloning, Site-Directed Mutagenesis (SDM)], SDS-PAGE, Western blotting, DNA extraction and purification, Agarose gel electrophoresis, PCR, Colony PCR, Plasmid PCR, Fusion PCR, Overlap extension PCR.
Protein purification: Ni-NTA chromatography, Gel filtration chromatography/Size exclusion chromatography, Pull-down (Immunoprecipitation) using lipid-coated agarose beads, Protein preparation using TCA method.
Mammalian Cell Culture: Maintaining cell lines, freezing, storage, transfection, producing pseudoviruses (HIV core pseudoviruses), harvesting pseudoviruses, expressing non-classical amino acid in pseudoviruses using orthogonal translation system.
Bioinformatics and statistical analysis: Sequence alignment (NCBI-BLAST), Primer design, ExPASy's ProtParam tool, T-COFFEE, MATLAB, PyMol, ChimeraX, SnapGene.

Awarded the Carl Storm International Diversity Award in Physical Virology GRC (January 22-27 2023) in Italy.
Awarded the Prime Minister Research Fellowship (PMRF), Lateral Entry, May 2020 Cycle.
Awarded the IAS-INSA-NASI Summer Research Fellowship (May 2018-July 2018): Fellowship for pursuing a summer research project in the lab of Dr. Rupinder Kaur, CDFD, Hyderabad
Awarded the CBSE CSSS Scholarship (2014-2019)
1st Prize: Puspangana Singh, Dibyendu Kumar Das. Mapping the Conformational Dynamics of MERS-CoV Spike Protein. LMB-UNIGE Graduate Life Sciences Symposium. LMB-MRC, Cambridge University (Virtual). July 8-10, 2020. [Poster].
1st Prize [Shakuntala Tiwari Memorial Research Excellence Award]: Puspangana Singh. Venturing into the real-time conformational landscape of the spike protein of coronaviruses for host cell entry. National Conference on Environmental and Industrial Biotechnology (NCEIB). Dr. Ambedkar Institute of Technology for Handicapped, Kanpur. November 10-12, 2022. [Student talk].
3rd Prize: Puspangana Singh, Priyanka Bhakt, Rupinder Kaur. Characterisation of Chaperonin Proteins in Candida glabrata. Summer Trainee Colloquium. Centre for DNA Fingerprinting and Diagnostics (CDFD, Hyderabad). June 29th, 2018. [Student talk].