卒業したメンバー
業績等は所属時で研究室に関連するものを抜粋
業績等は所属時で研究室に関連するものを抜粋
博士（工学）、2025年3月
修士（工学）、2022年3月
学士（工学）、2020年3月
日本学術振興会特別研究員DC2、2023年
数理物質科学研究群長表彰（後期課程）、2025年
Akira Nomoto, Kentaro Shiraki. Thermal aggregation of immunoglobulin G depending on the charge state of protein–polyelectrolyte complexes. International Journal of Biological Macromolecules, 139500 (2025)
Akira Nomoto, Kentaro Shiraki, Tsukuru Minamiki. Analysis of interactions between amino acids and monolayers of charged side chains. RSC Applied Interfaces (2025)
Md Motaher Hossain, Jannatul Morshed, Akira Nomoto, Seiya Tsujimura, Subbiah Alwarappan. Porous carbon-based electrochemical platform for direct electron transfer in myoglobin. Electroanalysis. 2024;36:e202300388 (2024)
野本 晃、白木 賢太郎「タンパク質の相転移と溶液の相互溶解に基づく液-液相分離現象」高分子 (2023)
野本 晃、白木 賢太郎「タンパク質の相分離を理解するためのアミノ酸指標」 Bioscience & Industry (2022)
Akira Nomoto, Suguru Nishinami, Kentaro Shiraki. Affinity of aromatic amino acid side chains in amino acid solvents. Biophysical Chemistry 106831, (2022)
Akira Nomoto, Suguru Nishinami, Kentaro Shiraki. Solubility Parameters of Amino Acids on Liquid–Liquid Phase Separation and Aggregation of Proteins. Frontiers in cell and developmental biology 9, 691052 (2021)
修士（工学）、2025年3月
学士（工学）、2023年3月
修士（工学）、2024年3月
Tomohiro Furuki, Azusa Togo, Hatsuho Usuda, Tomohiro Nobeyama, Atsushi Hirano, Kentaro Shiraki. Monovalent Ion Effect on Liquid–Liquid Phase Separation of Aqueous Polyphosphate–Salt Mixtures. The Journal of Physical Chemistry B.
Tomohiro Furuki, Tomohiro Nobeyama, Shunji Suetaka, Ryokei Matsui, Tatsuhiko Fukuoka, Munehito Arai, Kentaro Shiraki. Reentrant condensation of a multicomponent cola/milk system induced by polyphosphate. Food Chemistry: X, 101165 (2024)
Tomohiro Nobeyama, Tomohiro Furuki, Kentaro Shiraki. Phase-Diagram Observation of Liquid–Liquid Phase Separation in the Poly(l-lysine)/ATP System and a Proposal for Diagram-Based Application Strategy. Langmuir 39, 17043-17049 (2023)
日本学術振興会特別研究員PD、2021年4月から2024年3月
液-液相分離による液滴と外部物質の相互作用に基づいた炭酸同化酵素の活性化。特別研究員奨励費（代表）（2021-2024）
抗体医薬の常温での長期保存を可能にする先端ミセルナノ材料の開発。若手研究（代表）（2022-2025)
黄金の蝶々型ナノデバイスを用いた炭酸同化酵素の活性化。ハーモニック伊藤財団 助成事業（2023）
CEMS Rising Star Award in CEMSupra 2024
MBSJ2023 Best Science Pitch Award 2023
Tomohiro Furuki, Tomohiro Nobeyama, Shunji Suetaka, Ryokei Matsui, Tatsuhiko Fukuoka, Munehito Arai, Kentaro Shiraki. Reentrant condensation of a multicomponent cola/milk system induced by polyphosphate. Food Chemistry: X, 101165 (2024)
Tomohiro Nobeyama, Toya Yoshida, Kentaro Shiraki. Interfacial and intrinsic molecular effects on the phase separation/transition of heteroprotein condensates . International Journal of Biological Macromolecules 254 128095-128095 (2024)
Tomohiro Nobeyama, Tomohiro Furuki, Kentaro Shiraki. Phase-Diagram Observation of Liquid–Liquid Phase Separation in the Poly(L-lysine)/ATP System and a Proposal for Diagram-Based Application Strategy. Langmuir 39 (48), 17043-17049 (2023)
Tomohiro Nobeyama, Koji Tataka, Megumi Mori, Tatsuya Murakami, Yoichi Yamada, Kentaro Shiraki. Synthesis of Butterfly‐Like Shaped Gold Nanomaterial: For the Regulation of Liquid–Liquid Phase‐Separated Biomacromolecule Droplets. Small 19 (49), 2300362 (2023)
博士（工学）、2023年3月
修士（工学）、2020年3月
学士（工学）、2018年3月
日本学術振興会特別研究員DC1、2020年4月
数理物質科学研究科長賞、2023年3月
META body研究会・発起人
Ura T, Kagawa A, Sakakibara N, et al. Activation of L-lactate oxidase by the formation of enzyme assemblies through liquid-liquid phase separation. Sci Rep. 2023;13(1):1435. Published 2023 Jan 25.
Sakakibara N, Ura T, Mikawa T, Sugai H, Shiraki K. Transient formation of multi-phase droplets caused by the addition of a folded protein into complex coacervates with an oppositely charged surface relative to the protein. Soft Matter. 2023;19(25):4642-4650.
Ura T, Sakakibara N, Hirano Y, et al. Activation of oxidoreductases by the formation of enzyme assembly. Sci Rep. 2023;13(1):14381
Tomoto Ura, Tomoshi Kameda, Fina A Laksmi, Matsujiro Ishibashi, Tsutomu Arakawa, Kentaro Shiraki, Atsushi Hirano. Affinity of phenolic compounds for transition metal ions immobilized on cation-exchange columns. J Chromatogr A. 1676, 463277 (2022)
Tomoto Ura, Shunsuke Tomita, Kentaro Shiraki. Dynamic behavior of liquid droplets with enzyme compartmentalization triggered by sequential glycolytic enzyme reactions. Chemical Communications 57 (93), 12544-12547 (2021)
Tomoto Ura, Tsutomu Arakawa, Kentaro Shiraki. Insight into the protein salting-in mechanism of arginine, magnesium chloride and ethylene glycol: Solvent interaction with aromatic solutes. International Journal of Biological Macromolecules, 188, 670-677 (2021)
Kentaro Shiraki, Masahiro Mimura, Suguru Nishinami, Tomoto Ura. Effect of Additives on Liquid Droplets and Aggregates of Proteins. Biophys Rev. 12(2), 587-592 (2020)
浦 朋人、白木 賢太郎「酵素反応と液-液相分離」『相分離生物学の全貌』東京化学同人 2020年11月
浦 朋人、白木 賢太郎「液-液相分離による酵素連続反応：細胞内にある代謝の理解」『実験医学』羊土社 2019年11月号
Atsushi Hirano, Kazuki Iwashita, Tomoto Ura, Shun Sakuraba, Kentaro Shiraki, Tsutomu Arakawa, Tomoshi Kameda. Binding affinity of uncharged aromatic solutes for negatively charged resins is enhanced by cations through cation–pai interaction: the case of sodium ion and arginine. J. Chromato. A, 1595 97–107 (2019)
博士（工学）、2023年3月
修士（工学）、2020年3月
学士（工学）、2018年3月
日本学術振興会特別研究員DC1、2020年4月
数理物質科学研究科長賞、2023年3月
西奈美卓、白木賢太郎「タンパク質溶液の状態はシミュレーションできるのか?」分子シミュレーション学会誌“アンサンブル” Vol. 24, No. 4, October (2022)
Akira Nomoto, Suguru Nishinami, Kentaro Shiraki. Affinity of aromatic amino acid side chains in amino acid solvents. Biophysical Chemistry 106831, (2022)
Suguru Nishinami, Tsutomu Arakawa, Kentaro Shiraki. Classification of protein solubilizing additives by fluorescence assay. International Journal of Biological Macromolecules 203, 695-702 (2022)
Yoshitaka Nakauchi, Suguru Nishinami, Yusuke Murakami, Toshihiko Ogura, Hideaki Kano, Kentaro Shiraki. Opalescence Arising from Network Assembly in Antibody Solution. Molecular Pharmaceutics, (2022)
Yoshitaka Nakauchi, Suguru Nishinami, Kentaro Shiraki. Glass-like protein condensate for the long-term storage of proteins. International Journal of Biological Macromolecules 182, 162-167 (2021)
Akira Nomoto, Suguru Nishinami, Kentaro Shiraki. Solubility Parameters of Amino Acids on Liquid–Liquid Phase Separation and Aggregation of Proteins. Frontiers in cell and developmental biology 9, 691052 (2021)
Suguru Nishinami, Keiko Ikeda, Tamiko Nagao, A Hajime Koyama, Tsutomu Arakawa, Kentaro Shiraki. Aromatic interaction of hydantoin compounds leads to virucidal activities. Biophysical Chemistry, 106621(2021)
Taehun Hong, Kazuki Iwashita, Jeungmin Han, Suguru Nishinami, Akihiro Handa, Kentaro Shiraki. Aggregation of hen egg white proteins with additives during agitation. LWT 146, 111378(2021)
Yoshitaka Nakauchi, Suguru Nishinami, Kentaro Shiraki. Glass-like protein condensate for the long-term storage of proteins. International Journal of Biological Macromolecules 182, 162-167 (2021)
西奈美卓、大橋祐美子「プリオンと相分離現象」『相分離生物学の全貌』東京化学同人 2020年11月
西奈美卓、白木賢太郎「相分離生物学で理解するプリオンの存在意義」日本物理学会誌 Vol. 75, No. 4, (2020)
Kentaro Shiraki, Masahiro Mimura, Suguru Nishinami, Tomoto Ura. Effect of Additives on Liquid Droplets and Aggregates of Proteins. Biophys Rev. 12(2), 587-592 (2020)
Suguru Nishinami, Tomoshi Kameda, Tsutomu Arakawa, and Kentaro Shiraki. Hydantoin and Its Derivatives Reduce the Viscosity of Concentrated Antibody Formulations by Inhibiting Associations via Hydrophobic Amino Acid Residues. Ind. Eng. Chem. Res, 58, 36, 16296-16306 (2019)
Suguru Nishinami, Atsushi Hirano, Tsutomu Arakawa, and Kentaro Shiraki. Effects of allantoin and dimethyl sulfoxide on the thermal aggregation of lysozyme. Int. J. Biol. Macromol., 119, 180-185 (2018)
Shogo Oki, Suguru Nishinami, Kentaro Shiraki. Arginine suppresses opalescence and liquid–liquid phase separation in IgG solutions. Int. J. Biol. Macromol. 118, 1708-1712 (2018)
Suguru Nishinami, Shunsuke Yoshizawa, Tsutomu Arakawa and Kentaro Shiraki. Allantoin and hydantoin as new protein aggregation suppressors. Int. J. Biol. Macromol., 114, 497-503 (2018)
Shunsuke Tomita, Ayumi Matsuda, Suguru Nishinami, Ryoji Kurita, Kentaro Shiraki. One-step identification of antibody degradation pathways using fluo-rescence signatures generated by cross-reactive DNA-based arrays. Anal. Chem. 89, 7818-7822 (2017)
博士（工学）、2023年3月
味の素（株）から社会人課程博士
Hayato Nagano, Teruhisa Mannen, Yoshimi Kikuchi, Kentaro Shiraki. The pH-responsive precipitation–redissolution of the CspB fusion protein, CspB50TEV-Teriparatide, triggered by changes in secondary structure. Biochemistry and Biophysics Reports 33, 101435 (2023)
Hayato Nagano, Teruhisa Mannen, Yoshimi Kikuchi, KentaroShiraki. Solution design to extend the pH range of the pH-responsive precipitation of a CspB fusion protein. Protein Expression and Purification 195, 106091 (2022)
学士（工学）、2021年3月
修士（工学）、2023年3月
Ura T, Kagawa A, Sakakibara N, et al. Activation of L-lactate oxidase by the formation of enzyme assemblies through liquid-liquid phase separation. Sci Rep. 2023;13(1):1435. Published 2023 Jan 25.
Sakakibara N, Ura T, Mikawa T, Sugai H, Shiraki K. Transient formation of multi-phase droplets caused by the addition of a folded protein into complex coacervates with an oppositely charged surface relative to the protein. Soft Matter. 2023;19(25):4642-4650.
Ura T, Sakakibara N, Hirano Y, et al. Activation of oxidoreductases by the formation of enzyme assembly. Sci Rep. 2023;13(1):14381
学士（工学）、2022年3月
「タンパク質の集合・離散を制御するペプチドタグの開発」戦略的創造研究推進事業（さきがけ） 2022年度
Hiroka Sugai, Shunsuke Tomita, Sayaka Ishihara, Kentaro Shiraki, Ryoji Kurita. Damage-free evaluation of cultured cells based on multivariate analysis with a single-polymer probe. Chem. Commun., 58, 11083-11086 (2022)
学士（工学）、2022年9月
修士（工学）、2022年3月
学士（工学）、2020年3月
Yuto Akahoshi, Hiroka Sugai, Masahiro Mimura, Yoichi Shinkai, Ryoji Kurita, Kentaro Shiraki, Shunsuke Tomita. Phase-separation propensity of non-ionic amino acids in peptide-based complex coacervation systems. Biomacromolecules 24 (2), 704-713 (2023)
修士（工学）、2021年3月
学士（工学）、2019年3月
Yoshitaka Nakauchi, Suguru Nishinami, Yusuke Murakami, Toshihiko Ogura, Hideaki Kano, Kentaro Shiraki. Opalescence Arising from Network Assembly in Antibody Solution. Molecular Pharmaceutics, (2022)
Yoshitaka Nakauchi, Suguru Nishinami, Kentaro Shiraki. Glass-like protein condensate for the long-term storage of proteins. International Journal of Biological Macromolecules 182, 162-167 (2021)
修士（工学）、2021年3月
学士（工学）、2019年3月
学士（工学）、2021年3月
Yusuke Murakami, Shinichi Miyazaki, Yu Hayashi, Philippe Leproux, Kentaro Shiraki, and Hideaki Kano. Exploration of P granules using Multimodal Nonlinear Optical Imaging with multiplex CARS, SHG, and THG (coherent anti-Stokes Raman scattering, second harmonic generation, and third harmonic generation)
修士（工学）、2020年3月
学士（工学）、2018年3月
Keisuke Tsumura, WeiLin Hsu, Masahiro Mimura, Aiko Horiuchi, Kentaro Shiraki. Lowering the viscosity of a high-concentration antibody solution by protein–polyelectrolyte complex. Journal of Bioscience and Bioengineering, 133, 17-24 (2022)
Masahiro Mimura, Keisuke Tsumura, Ayumi Matsuda, Naoki Akatsuka, and Kentaro Shiraki. Effect of additives on liquid droplet of protein–polyelectrolyte complex for high-concentration formulations. J Chem Phys. 150, 064903 (2019)
学士（工学）、2020年3月
博士（工学）、2019年3月
修士（工学）、2016年3月
学士（工学）、2014年3月
日本学術振興会特別研究員DC2、2017年4月
H30年度数理物質科学研究科長賞
Chika Shibata, Kazuki Iwashita, Kentaro Shiraki. Salt-containing aqueous two-phase system shows predictable partition of proteins with surface amino acids residues. Int. J. Biol. Macromol., 133, 1182-1186 (2019).
Chika Shibata, Kazuki Iwashita, Kentaro Shiraki. Selective separation method of aggregates from IgG solution by aqueous two-phase system. Protein Expr Purif. 161, 57-62 (2019).
Atsushi Hirano, Kazuki Iwashita, Tomoto Ura, Shun Sakuraba, Kentaro Shiraki, Tsutomu Arakawa, Tomoshi Kameda. Binding affinity of uncharged aromatic solutes for negatively charged resins is enhanced by cations through cation–pai interaction: the case of sodium ion and arginine. J. Chromato. A, 1595 97–107 (2019).
白木賢太郎、岩下和輝、三村真大「タンパク質の共凝集と液-液相分離」（分担執筆）シーエムシー出版
Kazuki Iwashita, Akihiro Handa, Kentaro Shiraki. Coacervates and coaggregates: Liquid-liquid and liquid-solid phase transitions by native and unfolded protein complexes. Int. J. Biol. Macromol. 120, 10-18 (2018)
Kazuki Iwashita, Masahiro Mimura, Kentaro Shiraki. Control of aggregation, co-aggregation, and liquid droplet of proteins by small additives. Curr Pharm Biotechnol. 19, 946-955 (2018), review
Kazuki Iwashita, Akihiro Handa, Kentaro Shiraki. Co-aggregation of ovotransferrin and lysozyme. Food Hydrocolloids, 89, 416-424 (2019).
Taehun Hong, Kazuki Iwashita, Kentaro Shiraki. Viscosity Control of Protein Solution by Small Solutes: A Review. Current Protein and Peptide Science, 19, 746-758 (2018)
Atsushi Hirano, Kazuki Iwashita, Shun Sakuraba, Kentaro Shiraki, Tsutomu Arakawa, Tomoshi Kameda. Salt-dependent Elution of Uncharged Aromatic Solutes in Ion-exchange Chromatography. Journal of Chromatography A, 1546, 46-55 (2018)
岩下和輝、亀田倫史、白木賢太郎。カーボンナノチューブとタンパク質の相互作用。生物工学会誌, vol 95, 723-725 (2017)
Shogo Oki, Kazuki Iwashita, Masahiro Kimura, Hideaki Kano, Kentaro Shiraki. Mechanism of co-aggregation in a protein mixture with small additives. Int. J. Biol. Macromol., 107, 1428-1437 (2018)
Kazuki Iwashita, Akihiro Handa, Kentaro Shiraki. Co-aggregation of ovalbumin and lysozyme. Food Hydrocolloids, 67 206-215 (2017)
Taehun Hong, Kazuki Iwashita, Akihiro Handa, Kentaro Shiraki. Arginine prevents thermal aggregation of hen egg white proteins. Food Research International, 97, 272-279 (2017)
Kazuki Iwashita, Motoki Sumida, Kazuya Shirota, and Kentaro Shiraki. A recovery method of surimi wash-water protein by pH shift and heat treatment. Food Science and Technology Research, 22 (6), 743-749 (2016)
Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano. Arginine Suppresses the Adsorption of Lysozyme on Single-Wall Carbon Nanotube, Chemistry Letters, 45, 952-954 (2016)
Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano. Liquid chromatographic analysis of the interaction between amino acids and aromatic surfaces using single-wall carbon nanotubes. Langmuir. 31, 8923-8929 (2015)
Kazuki Iwashita, Naoto Inoue, Akihiro Handa, and Kentaro Shiraki. Thermal aggregation of hen egg white proteins in the presence of salts. Protein J. 34, 212-219 (2015)
白木賢太郎、岩下和輝「タンパク質の凝集：モデルと測定法」『微粒子分散・凝集ハンドブック』シーエムシー出版
博士（工学）、2019年3月
味の素（株）から社会人課程博士
Takahiro Nonaka, Noriko Tsurui, Teruhisa Mannem, Yoshimi Kikuchi, Kentaro Shiraki. Non-chromatographic purification of teriparatide with a pH-responsive CspB tag. Protein Expr. Purif. 155, 66-71 (2019)
Takahiro Nonaka, Noriko Tsurui, Teruhisa Mannem, Yoshimi Kikuchi, Kentaro Shiraki. A new pH-responsive peptide tag for protein purification.Protein Expression and Purification 146, 91-96 (2018)
Shogo Oki, Takahiro Nonaka, Kentaro Shiraki. Specific solubilization of aromatic impurities in culture media: Arg solution improves purification of pH-responsive tag CspB50 with Teriparatide. Protein Expression and Purification 146, 85-90 (2018)
修士（工学）、2019年3月
学士（工学）、2017年3月
Chika Shibata, Kazuki Iwashita, Kentaro Shiraki. Salt-containing aqueous two-phase system shows predictable partition of proteins with surface amino acids residues. Int. J. Biol. Macromol., 133, 1182-1186 (2019).
Chika Shibata, Kazuki Iwashita, Kentaro Shiraki. Selective separation method of aggregates from IgG solution by aqueous two-phase system. Protein Expr Purif. 161, 57-62 (2019).
修士（工学）、2019年3月
学士（工学）、2017年3月
「市販の蛍光色素を利用したパターン認識に基づくタンパク質および細胞識別法の開発」2018年 東日本分析化学若手交流会（優秀ポスター賞）
H30年度数理物質科学研究科長賞
日本学術振興会特別研究員DC2、2020年4月
Masahiro Mimura, Keisuke Tsumura, Ayumi Matsuda, Naoki Akatsuka, and Kentaro Shiraki. Effect of additives on liquid droplet of protein–polyelectrolyte complex for high-concentration formulations. J Chem Phys. 150, 064903 (2019)
国際特許 PTC/JP2018/027771
白木賢太郎、岩下和輝、三村真大「タンパク質の共凝集と液-液相分離」（分担執筆）シーエムシー出版
Kazuki Iwashita, Masahiro Mimura, Kentaro Shiraki. Control of aggregation, co-aggregation, and liquid droplet of proteins by small additives. Curr Pharm Biotechnol. 19, 946-955 (2018), review
Ayumi Matsuda, Masahiro Mimura, Takuya Maruyama, Takaaki Kurinomaru, Shiuhei Mieda, and Kentaro Shiraki. Liquid Droplet of Protein–Polyelectrolyte Complex for High-Concentration Formulations. Journal of Pharmaceutical Sciences, 107, 2713-2719 (2018)
Masahiro Mimura, Shunsuke Tomita, Ryoji Kurita, and Kentaro Shiraki. Array-based generation of response fingerprints with common fluorescent dyes for identification of proteins and cells. Anal Sci. 35, 99-102 (2019)
「タンパク質オリガミで自在にかごをつくる」（解説）
医療用タンパク質の複合体。特願2017-142905（テルモ株式会社、筑波大学）
博士（工学）、2018年3月
修士（工学）、2015年3月
学士（工学）、2013年3月（早期卒業）
笹川科学研究助成、2016年
Shunsuke Yoshizawa, Satoshi Ikawa, Katsuhisa Kitano Kentaro Shiraki. Biochemical mechanism of inactivation of the enzyme by low-temperature atmospheric plasma jet in aqueous solution.
Suguru Nishinami, Shunsuke Yoshizawa, Tsutomu Arakawa and Kentaro Shiraki. Allantoin and hydantoin as new protein aggregation suppressors. Int. J. Biol. Macromol., in press.
Tomohito Nakayama, Shunsuke Yoshizawa, Atsushi Hirano, Takeshi Tanaka, Kentaro Shiraki, Muneaki Hase. Vibrational Energy Transfer from Photo-Excited Carbon Nanotubes to Proteins Observed by Coherent Phonon Spectroscopy. Applied Physics Express, 10 (12), 125101 (2017)
Shunsuke Yoshizawa, Shogo Oki, Tsutomu, Arakawa, and Kentaro Shiraki. Trimethylamine N-oxide (TMAO) is a counteracting solute of benzyl alcohol for multi-dose formulation of immunoglobulin. Int. J. Biol. Macromol., 107, 984-989 (2018)
Shunsuke Yoshizawa, Tsutomu Arakawa, and Kentaro Shiraki. Thermal Aggregation of Human Immunoglobulin G in Arginine Solutions: Contrasting Effects of Stabilizers and Destabilizers, Int. J. Biol. Macromol., 104 (2017) 650–655
Shunsuke Yoshizawa, Tsutomu Arakawa, and Kentaro Shiraki. Effect of counter ions of arginine as an additive for the solubilization of protein and aromatic compounds. Int. J. Biol. Macromol. 91, 471-476 (2016)
Takumi Miyatake, Shunsuke Yoshizawa, Tsutomu Arakawa, Kentaro Shiraki. Charge state of arginine as an additive on heat-induced protein aggregation. Int. J. Biol. Macromol. 87, 563-569 (2016)
吉澤俊祐 、白木賢太郎「タンパク質の凝集剤としての塩・有機溶媒・高分子」生物工学、2015.5月号
Shunsuke Yoshizawa, Tsutomu Arakawa, and Kentaro Shiraki. Dependence of ethanol effects on protein charges. Int. J. Biological Macromolecules. 68, 169-172 (2014)
Eisuke Takai, Tsuyoshi Kitamura, Junpei Kuwabara, Satoshi Ikawa, Shunsuke Yoshizawa, Kentaro Shiraki, Hideya Kawasaki, Ryuichi Arakawa, and Katsuhisa Kitano. Chemical Modification of Amino Acids by Atmospheric-Pressure Cold Plasma in Aqueous Solution. J. Phys. D: Appl. Phys. 47, 285403 (2014)
Eisuke Takai, Shunsuke Yoshizawa, Daisuke Ejima, Tsutomu Arakawa, and Kentaro Shiraki. Synergistic Solubilization of Porcine Myosin in Physiological Salt Solution by Arginine. Int. J. Biological Macromolecules. 62, 674-651 (2013)
修士（工学）、2018年3月
学士（工学）、2016年3月
先導的研究者体験プログラム（ARE）優秀賞「卵白タンパク質の研究」
応用理工学類長賞、2016年3月
Taehun Hong, Kazuki Iwashita, Kentaro Shiraki. Viscosity Control of Protein Solution by Small Solutes: A Review
Taehun Hong, Kazuki Iwashita, Akihiro Handa, Kentaro Shiraki. Arginine prevents thermal aggregation of hen egg white proteins. Food Research International, 97, 272-279 (2017)
修士（工学）、2018年3月
学士（工学）、2016年3月
学修優秀賞（名取賞）2016年3月
Akihiro Endo, Takaaki Kurinomaru, and Kentaro Shiraki. Hyperactivation of serine proteases by the Hofmeister effect.
Akihiro Endo, Takaaki Kurinomaru, and Kentaro Shiraki. Hyperactivation of α-chymotrypsin by the Hofmeister effect. J. Mol. Catal. B: Enzymatic, 133, 432-438 (2016)
博士（工学）、2022年3月（社会人博士）
修士（工学）、2018年3月
学士（工学）、2016年3月
Shogo Oki, Suguru Nishinami, Yoshitaka Nakauchi, Toshihiko Ogura, Kentaro Shiraki. Arginine and its Derivatives Suppress the Opalescence of an Antibody Solution. Journal of Pharmaceutical Sciences, 111, 1126-1132 (2022)
Shogo Oki, Suguru Nishinami, Kentaro Shiraki. Arginine suppresses opalescence and liquid–liquid phase separation in IgG solutions. Int. J. Biol. Macromol. 118, 1708-1712 (2018)
Shogo Oki, Takahiro Nonaka, Kentaro Shiraki. Specific solubilization of aromatic impurities in culture media: Arg solution improves purification of pH-responsive tag CspB50 with Teriparatide. Protein Expression and Purification 146, 85-90 (2018)
Shogo Oki, Kazuki Iwashita, Masahiro Kimura, Hideaki Kano, Kentaro Shiraki. Mechanism of co-aggregation in a protein mixture with small additives. Int. J. Biol. Macromol., Int. J. Biol. Macromol., 107, 1428-1437 (2018)
Shunsuke Yoshizawa, Shogo Oki, Tsutomu, Arakawa, and Kentaro Shiraki. Trimethylamine N-oxide (TMAO) is a counteracting solute of benzyl alcohol for multi-dose formulation of immunoglobulin. Int. J. Biol. Macromol., 107, 984-989 (2018)
Shogo Oki, Tsutomu Arakawa, Kentaro Shiraki. The effects of n-acetyltryptophan and caprylic acid on protein aggregation. J. Biol. Macromol. 16, 3-7 (2016)
修士（工学）、2017年3月
学士（工学）、2015年3月
Masahiro Mimura, Keisuke Tsumura, Ayumi Matsuda, Naoki Akatsuka, and Kentaro Shiraki. Effect of additives on liquid droplet of protein–polyelectrolyte complex for high-concentration formulations. J. Chem. Phys.
Shunsuke Tomita, Ayumi Matsuda, Suguru Nishinami, Ryoji Kurita, Kentaro Shiraki. One-step identification of antibody degradation pathways using fluo-rescence signatures generated by cross-reactive DNA-based arrays. Anal. Chem. 89, 7818-7822 (2017)
医療用タンパク質の複合体。特願2016-205236（テルモ株式会社、筑波大学）
Ayumi Matsuda, Masahiro Mimura, Takuya Maruyama, Takaaki Kurinomaru, Shiuhei Mieda, and Kentaro Shiraki. Liquid Droplet of Protein–Polyelectrolyte Complex for High-Concentration Formulations. Journal of Pharmaceutical Sciences, 107, 2713-2719 (2018)
修士（工学）、2017年3月
学士（工学）、2015年3月
Takumi Miyatake, Shunsuke Yoshizawa, Tsutomu Arakawa, Kentaro Shiraki. Charge state of arginine as an additive on heat-induced protein aggregation. Int. J. Biol. Macromol. 87, 563-569 (2016)
博士（工学）、2015年3月（早期修了）
修士（工学）、2013年3月
学士（工学）、2011年3月
日本学術振興会特別研究員（DC1→PD）
高分子学会関東支部茨城地区若手の会2012年 最優秀ポスター賞
高分子学会関東支部茨城地区若手の会2011年 優秀ポスター賞
PSW2011最優秀ポスター賞
筑波大学大学院 数理物質科学研究科長表彰 博士 2015年3月
筑波大学大学院 数理物質科学研究科長表彰 修士 2013年3月
筑波大学 応用理工学類 第２回学修優秀賞（名取賞） 2011年3月
Takaaki Kurinomaru, Kengo Kuwada, Shunsuke Tomita, Tomoshi Kameda and Kentaro Shiraki. Noncovalent PEGylation through Protein-Polyelectrolyte Interaction: Kinetic Experiment and Molecular Dynamics Simulation, J. Phys. Chem. B. 121, 6785-6791 (2017)
Kengo Kuwada, Takaaki Kurinomaru, Shunsuke Tomita, and Kentaro Shiraki. Noncovalent PEGylation-based enzyme switch in physiological saline conditions using quaternized polyamines. Colloid and Polymer Science, 296, 1551-1556 (2016)
Takaaki Kurinomaru and Kentaro Shiraki. Aggregative protein-polyelectrolyte complex for high-concentration formulation of protein drug. Int. J. Biol. Macromol. in press
Kentaro Shiraki, Takaaki Kurinomaru, and Shunsuke Tomita. Wrap-and-Strip Technology of Protein-Polyelectrolyte Complex for Biomedical Application. Curr. Med. Chem., 23, 276-289 (2016), review
Shunsuke Izaki, Takaaki Kurinomaru, Kenji Handa, Tomoaki Kimoto, and Kentaro Shiraki. Stress Tolerance of Antibody-Poly(amino acid) Complexes for Improving the Stability of High Concentration Antibody Formulations. J. Pharm. Sci. 104, 2457-2463 (2015)
Takuya Maruyama, Shunsuke Izaki, Takaaki Kurinomarua, Kenji Handa, Tomoaki Kimoto, and Kentaro Shiraki. Protein-poly(amino acid) precipitation stabilizes a therapeutic protein of L-asparaginase against physicochemical stress. J. Biosci. Bioeng.120, 720-723 (2015)
栗之丸隆章「高分子電解質で制御するタンパク質の物性と機能」『生物工学』2015.5月号
栗之丸隆章、白木賢太郎「タンパク質沈殿-再溶解法による高濃度タンパク質医薬品の調製」『化学と生物』vol 53-6, 2015
Shunsuke Izaki, Takaaki Kurinomaru, Takuya Maruyama, Kenji Handa, Tomoaki Kimoto and Kentaro Shiraki. Feasibility of Antibody-Poly(amino acid) Complexes: Preparation of High-concentration Antibody Formulations and their Pharmaceutical Properties. J. Pharm. Sci. J. Pharm. Sci. 104, 1929-1937 (2015)
Takaaki Kurinomaru, Tomoshi Kameda, and Kentaro Shiraki. Effects of hydrophobicity and multivalency of polyamines on enzyme hyperactivation of alpha-chymotrypsin. J. Mol. Catal. B: Enzymatic 115, 135-139 (2015)
Takaaki Kurinomaru and Kentaro Shiraki. Non-covalent PEGylation of L-Asparaginase Using PEGylated Polyelectrolyte. J. Pharm. Sci. 104, 587-592 (2015)
Takaaki Kurinomaru, Takuya Maruyama, Shunsuke Izaki, Kenji Handa, Tomoaki Kimoto, and Kentaro Shiraki. Protein-Poly(amino acid) Complex Precipitation for High-concentration Protein Formulation. J. Pharm. Sci. 103, 2248-2254 (2014)
Takaaki Kurinomaru, Shunsuke Tomita, Yoshihisa Hagihara, and Kentaro Shiraki. Enzyme Hyperactivation System Based on Generated Reaction Field by Polyelectrolyte. Langmuir 30, 3826-3831 (2014)
特許、振とうストレス耐性を有するタンパク質水性懸濁剤。特願2014-219536 （テルモ株式会社）
タンパク質水溶性懸濁剤。PCT/JP2014/78667（テルモ株式会社）
耐熱性を有するタンパク質水性懸濁剤。特願2013-223774（テルモ株式会社）
振とうストレス耐性を有するタンパク質水性懸濁剤。特願2013-223775（テルモ株式会社）
耐酸化性を有するタンパク質水性懸濁剤。特願2013-223778（テルモ株式会社）
凝集抑制性を有するタンパク質水性懸濁剤。特願2013-223779（テルモ株式会社）
流動性を有するタンパク質水性懸濁剤。特願2013-223780（テルモ株式会社）
栗之丸隆章、白木賢太郎「高分子電解質をトリガーに用いる酵素機能の制御：トグルスイッチと超活性化」Bioscience & Industry vol. 71, 318-321 (2013)
Takaaki Kurinomaru, Shunsuke Tomita, Shinpei Kudo, Sumon Ganguli, Yukio Nagasaki, and Kentaro Shiraki. Improved Complementary Polymer Pair System: Switching for Enzyme Activity by PEGylated Polymers. Langmuir 28, 4334-4338 (2012).
栗之丸隆章「人工酵素と天然酵素でつくる代謝反応」現代化学 FLASH, 505, 4 (2013)
栗之丸隆章「特別研究員へのインタビュー」生物工学会誌 vol. 91, 12月号 (2013)
修士（工学）、2016年3月
学士（工学）、2012年3月
ウィスコンシン大学マディソン校薬学部に留学、2014年-2015年
Masato Shimada, Eisuke Takai, Daisuke Ejima, Tsutomu Arakawa and Kentaro Shiraki. Heat-induced formation of myosin oligomer-soluble filament complex in high salt solution. International Journal of Biological Macromolecules 73, 17-22 (2015)
修士（工学）、2016年3月
学士（工学）、2014年3月
Tsutomu Arakawa, Masao Tokunaga, Takuya Maruyama, Kentaro Shiraki. Two Elution Mechanisms of MEP Chromatography. Current Protein & Peptide Science (review)
Takuya Maruyama, Shunsuke Izaki, Takaaki Kurinomarua, Kenji Handa, Tomoaki Kimoto, and Kentaro Shiraki. Protein-poly(amino acid) precipitation stabilizes a therapeutic protein of L-asparaginase against physicochemical stress. J. Biosci. Bioeng.120, 720-723 (2015)
Shunsuke Izaki, Takaaki Kurinomaru, Takuya Maruyama, Kenji Handa, Tomoaki Kimoto and Kentaro Shiraki. Feasibility of Antibody-Poly(amino acid) Complexes: Preparation of High-concentration Antibody Formulations and their Pharmaceutical Properties. J. Pharm. Sci. 104, 1929-1937 (2015)
Atsushi Hirano, Takuya Maruyama, Kentaro Shiraki, Tsutomu Arakawa, and Tomoshi Kameda. Mechanism of Protein Desorption from 4-Mercaptoethylpyridine Resins by Arginine Solutions. J. Chromatogr. A, 1373, 141-148 (2014)
Takaaki Kurinomaru, Takuya Maruyama, Shunsuke Izaki, Kenji Handa, Tomoaki Kimoto, and Kentaro Shiraki. Protein-Poly(amino acid) Complex Precipitation for High-concentration Protein Formulation. J. Pharm. Sci. 103, 2248-2254 (2014)
特許、振とうストレス耐性を有するタンパク質水性懸濁剤。特願2014-219536 （テルモ株式会社）
タンパク質水溶性懸濁剤。PCT/JP2014/78667（テルモ株式会社）
耐熱性を有するタンパク質水性懸濁剤。特願2013-223774（テルモ株式会社）
振とうストレス耐性を有するタンパク質水性懸濁剤。特願2013-223775（テルモ株式会社）
耐酸化性を有するタンパク質水性懸濁剤。特願2013-223778（テルモ株式会社）
凝集抑制性を有するタンパク質水性懸濁剤。特願2013-223779（テルモ株式会社）
流動性を有するタンパク質水性懸濁剤。特願2013-223780（テルモ株式会社）
修士（工学）、2016年3月
学士（工学）、2014年3月
Takaaki Kurinomaru, Kengo Kuwada, Shunsuke Tomita, Tomoshi Kameda and Kentaro Shiraki. Noncovalent PEGylation through Protein-Polyelectrolyte Interaction: Kinetic Experiment and Molecular Dynamics Simulation, J. Phys. Chem. B. 121, 6785-6791 (2017)
Kengo Kuwada, Takaaki Kurinomaru, Shunsuke Tomita, and Kentaro Shiraki. Noncovalent PEGylation-based enzyme switch in physiological saline conditions using quaternized polyamines. Colloid and Polymer Science, Colloid and Polymer Science, 296, 1551-1556 (2016)
修士（工学）、2015年3月
学士（工学）、2013年3月
Kentaro Shiraki, Shunsuke Tomita, and Naoto Inoue. Small Amine Molecules: Solvent Design Toward Facile Improvement of Protein Stability Against Aggregation and Inactivation. Curr. Pharm. Biotechnol. 17, 116-125 (2016), review
特許、ケラチンの熱凝集抑制剤。特願2014-254301（株式会社ミルボン）
Kazuki Iwashita, Naoto Inoue, Akihiro Handa, and Kentaro Shiraki. Thermal aggregation of hen egg white proteins in the presence of salts. Protein J. 34, 212-219 (2015)
Naoto Inoue, Eisuke Takai, Tsutomu Arakawa, and Kentaro Shiraki. Specific decrease in solution viscosity of antibodies by arginine for therapeutic formulations. Molecular Pharmaceutics. Jun 2;11(6):1889-96 (2014)
Naoto Inoue, Eisuke Takai, Tsutomu Arakawa, and Kentaro Shiraki, Arginine and Lysine Reduce the High Viscosity of Serum Albumin Solutions for Pharmaceutical Injection, J. Biosci. Bioeng. 117, 539-543 (2014)
「高濃度γグロブリン製剤の粘度低下方法」特許第6179939（筑波大学）
井上直人、白木賢太郎「難水溶性分子の溶解性を改善するアルギニン」Bioindustry vol. 30, 22-28 (2013)
修士（工学）、2015年3月
学士（工学）、2013年3月
Katsuhisa Murakami, Tianchen Dong, Yuya Kajiwara, Takaki Hiyama, Teppei Takahashi, Eisuke Takai, Gai Ohashi, Kentaro Shiraki, and Jun-ichi Fujita. Synthesis of graphene nanoribbons from amyloid templates by gallium vapor-assisted solid-phase graphitization. Appl. Phys. Lett. 104, 243101 (2014)
Eisuke Takai, Gai Ohashi, Ryuichi Ueki, Yoichi Yamada, Jun-ichi Fujita, Kentaro Shiraki. Scanning Electron Microscope Imaging of Amyloid Fibrils. American Journal of Biochemistry and Biotechnology 10, 31-39, (2014)
Eisuke Takai, Gai Ohashi, Tomonori Yoshida, Karin Margareta Sorgjerd, Tamotsu Zako, Mizuo Maeda, Katsuhisa Kitano, Kentaro Shiraki, Degeneration of Amyloid-ß Fibrils Caused by Exposure to Low-Temperature Atmospheric-Pressure Plasma in Aqueous Solution, Applied Physics Letters, 104, 023701 (2014)
博士（工学）2014年3月（早期）
修士（工学）2012年3月
学士（工学）2010年3月
日本学術振興会特別研究員DC1
筑波大学大学院数理物質科学研究科長賞 修士
Masato Shimada, Eisuke Takai, Daisuke Ejima, Tsutomu Arakawa and Kentaro Shiraki. Heat-induced formation of myosin oligomer-soluble filament complex in high salt solution. International Journal of Biological Macromolecules 73, 17–22 (2015)
Toru Nakayama, Taro Sakuraba, Shunsuke Tomita, Akira Kaneko, Eisuke Takai, Kentaro Shiraki, Kentaro Tashiro, Noriyuki Ishii, Yuri Hasegawa, Yoichi Yamada, Reiji Kumai, and Yohei Yamamoto. Charge-Separated Fmoc-Peptide beta-Sheets: Sequence-Secondary Structure Relationship for Arranging Charged Side Chains on Both Sides .Asian J. Org. Chem., 3, 1182-1188 (2014)
Katsuhisa Murakami, Tianchen Dong, Yuya Kajiwara, Takaki Hiyama, Teppei Takahashi, Eisuke Takai, Gai Ohashi, Kentaro Shiraki, and Jun-ichi Fujita. Synthesis of graphene nanoribbons from amyloid templates by gallium vapor-assisted solid-phase graphitization. Appl. Phys. Lett. 104, 243101 (2014)
Naoto Inoue, Eisuke Takai, Tsutomu Arakawa, and Kentaro Shiraki. Specific decrease in solution viscosity of antibodies by arginine for therapeutic formulations. Molecular Pharmaceutics. Jun 2;11(6):1889-96 (2014)
Eisuke Takai, Ken Uda, Shuhei Matsushita, Yui Shikiya, Yoichi Yamada, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits Amyloid Fibrillation of Lysozyme and Directs the Formation of Small Worm-like Aggregates through Non-Covalent Interactions. Biotechnol. Prog. 30, 470-478 (2014)
Eisuke Takai, Ken Uda, Tomonori Yoshida, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits the Fibrillisation and Cytotoxicity of Amyloid-b 40 and 42: Implications for the Contribution of the Thiophilic Interaction. Physical Chemistry Chemical Physics 16, 3566 - 3572 (2014)
Naoto Inoue, Eisuke Takai, Tsutomu Arakawa, and Kentaro Shiraki, Arginine and Lysine Reduce the High Viscosity of Serum Albumin Solutions for Pharmaceutical Injection, Journal of Bioscience and Bioengineering, 117, 539-543 (2014)
Eisuke Takai, Gai Ohashi, Ryuichi Ueki, Yoichi Yamada, Jun-ichi Fujita, Kentaro Shiraki. Scanning Electron Microscope Imaging of Amyloid Fibrils. American Journal of Biochemistry and Biotechnology 10, 31-39, (2014)
Eisuke Takai, Gai Ohashi, Tomonori Yoshida, Karin Margareta Sorgjerd, Tamotsu Zako, Mizuo Maeda, Katsuhisa Kitano, Kentaro Shiraki, Degeneration of Amyloid-beta Fibrils Caused by Exposure to Low-Temperature Atmospheric-Pressure Plasma in Aqueous Solution, Applied Physics Letters, 104, 023701 (2014)
「高濃度γグロブリン製剤の粘度低下方法」特許第6179939（筑波大学）
Eisuke Takai, Shunsuke Yoshizawa, Daisuke Ejima, Tsutomu Arakawa, and Kentaro, Synergistic Solubilization of Porcine Myosin in Physiological Salt Solution by Arginine. International Journal of Biological Macromolecules 62, 647-51 (2013)
Eisuke Takai, Satoshi Ikawa, Katsuhisa Kitano, Junpei Kuwabara, Kentaro Shiraki, Molecular mechanism of plasma sterilization in solution with the reduced pH method: importance of permeation of HOO radicals into the cell membrane, Journal of Physics D: Applied Physics 46, 295402 (2013)
Eisuke Takai, Katsuhisa Kitano, Junpei Kuwabara, Kentaro Shiraki, Protein Inactivation by Low-Temperature Atmospheric Pressure Plasma in Aqueous Solution, Plasma Processes and Polymers 9, 77-82 (2012)
Eisuke Takai, Atsushi Hirano, Kentaro Shiraki, Effects of alkyl chain length of gallate on self-association and membrane-binding. J. Biochem. 150, 165-171 (2011)
高井英輔「タンパク質でハンバーグからプラズマまで」筑波大学広報刊行誌Students, 652, 9 (2014)
高井英輔「コロイドで作ったミクロな分子模型」現代化学 FLASH, 502, 4 (2013)
高井英輔,日本生物物理学会会誌 生物物理,「若手の声：東日本大震災を研究室で被災して」51, 4, 198-199 (2011)
修士（工学）、2013年3月
学士（工学）、2011年3月
Atsushi Hirano, Tomoshi Kameda, Daisuke Shinozaki, Tsutomu Arakawa, Kentaro Shiraki. Molecular Dynamics Simulation of Arginine-Assisted Solubilization of Caffeic Acid: Intervention in the Interaction. J. Phys. Chem. B 117, 7518-7527 (2013)
修士（工学）、2013年3月
学士（工学）、2011年3月
Eisuke Takai, Ken Uda, Shuhei Matsushita, Yui Shikiya, Yoichi Yamada, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits Amyloid Fibrillation of Lysozyme and Directs the Formation of Small Worm-like Aggregates through Non-Covalent Interactions. Biotechnol. Prog. 30, 470-478 (2014)
Yui Shikiya, Shunsuke Tomita, Tsutomu Arakawa, and Kentaro Shiraki. Arginine inhibits proteins adsorption on polystyrene surface. PLoS One 8, e70762 (2013)
修士（工学）、2013年3月
学士（工学）、2011年3月（早期卒業）
博士（工学）、2011年7月（早期修了）
修士（工学）、2009年3月
学士（工学）、2007年3月
日本学術振興会博士研究員、2012年4月
第21回日本MRS学術シンポジウム、奨励賞、2011年
財団法人日本科学協会、平成23年度笹川科学研究助成、2011年
筑波大学、つくばダイアモンド研究奨励費、2010年
Second International Symposium on Atomic Technologyポスター賞
日本蛋白質科学会ポスター賞2010
Shunsuke Tomita, Yukio Nagasaki, and Kentaro Shiraki. Different mechanisms of action of poly(ethylene glycol) and arginine on thermal inactivation of lysozyme and ribonuclease A. Biotechnology and Bioengineering, 109, 2543-2552 (2012)
Takaaki Kurinomaru, Shunsuke Tomita, Shinpei Kudo, Sumon Ganguli, Yukio Nagasaki, and Kentaro Shiraki. Improved complementary polymer pair system: Switching for enzyme activity by PEGylated polymers. Langmuir 28, 4334-4338 (2012)
Len Ito, Masaki Okumura, Kohsaku Tao, Yusuke Kasai, Shunsuke Tomita, Akiko Oosuka, Hidetoshi Yamada, Tomohisa Shibano, Kentaro Shiraki, Takashi Kumasaka, Hiroshi Yamaguchi. Glutathione ethylester, a novel protein refolding reagent, enhances both the efficiency of refolding and correct disulfide formation. Protein J. 31, 499-502 (2012)
Shunsuke Tomita, Yukio Nagasaki, and Kentaro Shiraki. Different mechanisms of action of poly(ethylene glycol) and arginine on thermal inactivation of lysozyme and ribonuclease A. Biotechnology and Bioengineering, 109, 2543-2552 (2012)
Shunsuke Tomita and Kentaro Shiraki. Poly(acrylic acid) is a common non-competitive inhibitor for cationic enzymes with high affinity and reversibility. Journal of Polymer Science Part A: Polymer Chemistry. 49, 3835-3841 (2011)
Shunsuke Tomita, Hiroki Yoshikawa, and Kentaro Shiraki. Arginine controls heat-induced cluster-cluster aggregation of lysozyme at around the isoelectric point. Biopolymers, 95, 695-701 (2011)
Shunsuke Tomita and Kentaro Shiraki. Why do solution additives suppress the heat-induced inactivation of proteins? Biotechnol. Prog., 27, 855-862 (2011)
冨田峻介、白木賢太郎「タンパク質の劣化を抑制する溶液添加剤：非共有結合的な凝集と共有結合的な修飾」極限環境生物学会誌, 9:81-89 (2010)
Shunsuke Tomita, Len Ito, Hiroshi Yamaguchi, Gen-ichi Konishi, Yukio Nagasaki, and Kentaro Shiraki. Enzyme switch by complementary polymer pair system (CPPS). Soft Matter 6, 5320-5326 (2010)
Sumon Ganguli, Keitaro Yoshimoto, Shunsuke Tomita, Hiroshi Sakuma, Tsuneyoshi Matsuoka, Kentaro Shiraki, and Yukio Nagasaki. Improving the Heat Resistance of Ribonuclease A by the Addition of Poly(N,N-diethylaminoethyl methacrylate)-graft-poly(ethylene glycol) (PEAMA-g-PEG). Macromolecular Bioscience, 10, 853-859 (2010)
Sumon Ganguli, Keitaro Yoshimoto, Shunsuke Tomita, Hiroshi Sakuma, Tsuneyoshi Matsuoka, Kentaro Shiraki, and Yukio Nagasaki. Regulation of Lysozyme Activity Based on Thermotolerant Protein/Smart Polymer Complex Formation. J. Am. Chem. Soc., 131, 6549-6553 (2009)
蛋白質アーカイブ#041「タンパク質熱凝集を抑制する化合物」松岡 常吉、冨田 峻介、平野 篤. 公開日 2008/6/30
Shunsuke Tomita, Hiroyuki Hamada, Yukio Nagasaki, and Kentaro Shiraki. Synergistic effect of polyethylene glycol with arginine on the prevention of heat-induced aggregation of lysozyme. J. Physics: Conference Series. 06:012022-012026 (2008)
蛋白質を含む液状組成物中における蛋白質の安定化方法、特許5119545号（筑波大学）
Tsuneyoshi Matsuoka, Shunsuke Tomita, Hiroyuki Hamada, and Kentaro Shiraki. Amidated Amino Acids Are Prominent Additives to Prevent Heat-Induced Aggregation of Lysozyme. J. Biosci. Biochem. vol. 103, 440-443 (2007)
修士（工学）、2012年3月
学士（工学）、2010年3月
修士（工学）、2012年3月
学士（工学）、2010年3月
Hiroki Yoshikawa, Atsushi Hirano, Tsutomu Arakawa, and Kentaro Shiraki. Effects of alcohol on the solubility and structure of native and disulfide-modified bovine serum albumin. Int. J. Biol. Macromol. 50, 1286-1291 (2012)
Atsushi Hirano, Hiroki Yoshikawa, Shuhei Matsushita, Yoichi Yamada, and Kentaro Shiraki. Adsorption and disruption of lipid bilayers by nanoscale protein aggregates. Langmure 28, 3887-3895 (2012)
Hiroki Yoshikawa, Atsushi Hirano, Tsutomu Arakawa, and Kentaro Shiraki. Mechanistic insights into protein precipitation by alcohol. Int. J. Biological Macromolecules 50, 865-871 (2012)
Shunsuke Tomita, Hiroki Yoshikawa, and Kentaro Shiraki. Arginine controls heat-induced cluster-cluster aggregation of lysozyme at around the isoelectric point. Biopolymers, 95, 695-701 (2011).
学士（工学）、2012年3月
学士（工学）、2012年3月
Shunsuke Tomita, Yumiko Tanabe, and Kentaro Shiraki. Oligoethylene glycols prevent thermal aggregation of alpha-chymotrypsin in a temperature-dependent manner: implications for design guidelines. Biotechnol. Prog. 29, 1325-1330 (2013)
博士（工学）（早期修了）、2010年3月
修士（工学）、2008年3月
学士（工学）、2006年3月
筑波大学数理物質科学研究科長賞 博士
日本学術振興会特別研究員DC1→博士研究員PD
ホソカワ粉体工学振興財団・研究者育成助成
Atsushi Hirano, Kentaro Shiraki, and Tsutomu Arakawa. Polyethylene glycol behaves like weak organic solvent. Biopolymers 97, 117-122 (2011)
Ryosuke Ariki, Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Arginine increases solubility of alkyl gallates through interaction with the aromatic ring. J. Biochem. 149, 389-394 (2011)
平野篤、白木賢太郎「姿をかえるタンパク質」生物工学, 89, 404-407 (2011)
Ryosuke Ariki, Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Drug solubilization effect of lauroyl-L-glutamate, J. Biochemistry, 151, 27-33 (2012)
Atsushi Hirano, Kentaro Shiraki, Takako Niikura, Tsutomu Arakawa and Yoshiko Kita. Structure of three Humanin peptides with different activities upon interaction with liposome. International Journal of Biological Macromolecules. 48, 360-363 (2011)
Atsushi Hirano, Ken Uda, Yutaka Maeda, Takeshi Akasaka, and Kentaro Shiraki. One-Dimensional Protein-Based Nanoparticles Induce Lipid Bilayer Disruption: Carbon Nanotube Conjugates and Amyloid Fibrils. Langmuir, 26, 17256-17259 (2010)
Atsushi Hirano, Tomoshi Kameda, Tsutomu Arakawa, Kentaro Shiraki. Arginine-Assisted Solubilization System for Drug Substances: Solubility Experiment and Simulation. J. Phys. Chem. B, 114, 13455-13462 (2010)
Atsushi Hirano, Ken Uda, Yutaka Maeda, Takeshi Akasaka, and Kentaro Shiraki. One-Dimensional Protein-Based Nanoparticles Induce Lipid Bilayer Disruption: Carbon Nanotube Conjugates and Amyloid Fibrils. Langmuir, 26, 17256-17259 (2010)
Atsushi Hirano, Yutaka Maeda, Xiaofei Yuan, Ryuuichi Ueki, Yosuke Miyazawa, Jun-ichi Fujita, Takeshi Akasaka, and Kentaro Shiraki. Controlled dispersion and purification of protein-CNT conjugates by guanidine hydrochloride. Chemistry, 16, 12221-12228 (2010)
Atsushi Hirano, Kentaro Shiraki, Takako Niikura, Tsutomu Arakawa and Yoshiko Kita. Structure changes of natively disordered Humanin in the presence of lipid. International Journal of Biometrics. 46, 375-379 (2010)
Atsushi Hirano, Hiroko Tokunaga, Masao Tokunaga, Tsutomu Arakawa, and Kentaro Shiraki. The solubility of nucleobases in aqueous arginine solutions. Archives of Biochemistry and Biophysics. 497, 90-96 (2010)
Atsushi Hirano, Yutaka Maeda, Takeshi Akasaka, and Kentaro Shiraki. Mechanism of enhanced dispersion of single-walled carbon nanotubes with proteins by alcohols and chaotropes. Japanese Journal of Applied Physics, 49, 06GJ10 (2010)
Akiko Fujimoto, Atsushi Hirano, and Kentaro Shiraki. Ternary system of solution additives with arginine and salt for refolding of beta-galactosidase. Protein J. 29, 161-166 (2010)
Kentaro Shiraki, Atsushi Hirano, Yoshiko Kita, A. Hajime Koyama and Tsutomu Arakawa. Potential application of arginine in interaction analysis. review, Drug Discoveries and Therapeutics, review, 4, 326-333 (2010)
Kotaro Makino, Atsushi Hirano, Kentaro Shiraki, Yutaka Maeda, Muneaki Hase. Ultrafast vibrational motion of carbon nanotubes in different pH environments. Physical Review B. 80, 245428 (2009)
特許、水系媒体中へのカーボンナノチューブの分散方法。特願2009-245773（筑波大学）
特許、水系溶媒中へのカーボンナノチューブの分散方法。特願2008-207851、特許第5224515号（筑波大学）
Tsutomu Arakawa, Atsushi Hirano, Kentaro Shiraki, Yoshiko Kita and A. Hajime Koyama. Stabilizing and destabilizing effects of arginine on deoxyribonucleic acid. Int. J. Biological Macromolecules. 46, 217-222 (2010)
Atsushi Hirano, Yutaka Maeda, Takeshi Akasaka, and Kentaro Shiraki, Synergistically Enhanced Dispersion of Native Protein-Carbon Nanotube Conjugates by Fluoroalcohols in Aqueous Solution. Chem. Eur. J., 15, 9905-9910 (2009)
Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Application of arginine to increse the solubility of poorly water-soluble compounds. J. Proteom. Bioinform., 2, 174 (2008)
蛋白質アーカイブ#041「タンパク質熱凝集を抑制する化合物」松岡 常吉、冨田 峻介、平野 篤. 公開日 2008/6/30
Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Arginine increases the solubility of coumarin: Comparison with salting-in and salting-out additives. J. Biochem. 144, 363-369 (2008)
Atsushi Hirano, Hiroyuki Hamada, and Kentaro Shiraki. Trans-cyclohexanediamines prevent thermal inactivation of protein: Role of hydrophobic and electrostatic interactions. Protein J. 27, 253-257 (2008)
Atsushi Hirano, Hiroyuki Hamada, Tatsunori Okubo, Takumi Noguchi, Hiroki Higashibata, and Kentaro Shiraki. Correlation between thermal aggregation and stability of lysozyme with salts described by molar surface tension increment: an exceptional propensity of ammonium salts as aggregation suppressor. Protein J. 26, 423-433 (2007)
白木 賢太郎、平野 篤、浜田 寛之. タンパク質凝集抑制剤の構造. 生物工学会誌 vol. 84, No. 10, 395-397 (2006)
修士（工学）、2011年3月
学士（工学）、2009年3月
Kentaro Shiraki and Soichiro Kayano, Thermal-Assisted Refolding: Dilution folding initiated at high temeprature. Curr. Pharm. Biotech. 11, 306-308 (2010).
特許、タンパク質のリフォールディング組成物。特願2009-018930
修士（工学）、2011年3月
学士（工学）、2009年3月
Ryosuke Ariki, Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Arginine increases solubility of alkyl gallates through interaction with the aromatic ring. J. Biochem. 149, 389-394 (2011).
Ryosuke Ariki, Atsushi Hirano, Tsutomu Arakawa, Kentaro Shiraki. Drug solubilization effect of lauroyl-L-glutamate, J. Biochemistry, 151, 27-33 (2012).
修士（工学）、2011年3月
Eisuke Takai, Ken Uda, Shuhei Matsushita, Yui Shikiya, Yoichi Yamada, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits Amyloid Fibrillation of Lysozyme and Directs the Formation of Small Worm-like Aggregates through Non-Covalent Interactions. Biotechnol. Prog. 30, 470-478 (2014)
Atsushi Hirano, Hiroki Yoshikawa, Shuhei Matsushita, Yoichi Yamada, and Kentaro Shiraki. Adsorption and disruption of lipid bilayers by nanoscale protein aggregates. Langmure 28, 3887-3895 (2012)
修士（工学）、2010年3月
学士（工学）、2008年3月
Eisuke Takai, Ken Uda, Shuhei Matsushita, Yui Shikiya, Yoichi Yamada, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits Amyloid Fibrillation of Lysozyme and Directs the Formation of Small Worm-like Aggregates through Non-Covalent Interactions. Biotechnol. Prog. 30, 470-478 (2014)
Eisuke Takai, Ken Uda, Tomonori Yoshida, Tamotsu Zako, Mizuo Maeda, and Kentaro Shiraki. Cysteine Inhibits the Fibrillisation and Cytotoxicity of Amyloid-b 40 and 42: Implications for the Contribution of the Thiophilic Interaction. Physical Chemistry Chemical Physics 16, 3566 - 3572 (2014)
特許、βアミロイド線維生成阻害剤。特願2009-245387
修士（工学）、2010年3月
学士（工学）、2008年3月
リフォールディングキット
Akiko Fujimoto, Atsushi Hirano, and Kentaro Shiraki. Ternary system of solution additives with arginine and salt for refolding of beta-galactosidase. Protein J. 29, 161-166 (2010).
特許、タンパク質のリフォールディング組成物。特許出願2009年1月30日、特願2009-018930
学士（工学）、2009年3月
学士（工学）、2007年3月
学士（修士）、2009年3月
学士（工学）、2009年3月
博士（工学）、2008年3月
筑波大学大学院数理物質科学研究科長賞、博士課程
Tsuneyoshi Matsuoka, Hiroyuki Hamada, Koji Matsumoto, and Kentaro Shiraki. Indispensable structure of solution additives that prevent misfolding for heating and refolding of lysozyme. Biotechnol. Prog. 25, 1515-1524 (2009)
Hiroyuki Hamada, Tsutomu Arakawa, and Kentaro Shiraki. Effect of additives on protein aggregation. Curr. Pharm. Biotechnol. 10, 400-407 (2009)
蛋白質アーカイブ#035「タンパク質リフォールディングの標準的な方法」浜田寛之、松岡常吉. 公開日 2008/6/9
Hiroyuki Hamada, Ryouta Takahashi, Takumi Noguchi, and Kentaro Shiraki. The differences in the effect of solution additives on heat- and refolding-induced aggregation. Biotechnol. Prog. 24, 436-443 (2008)
Atsushi Hirano, Hiroyuki Hamada, and Kentaro Shiraki. Trans-cyclohexanediamines prevent thermal inactivation of protein: Role of hydrophobic and electrostatic interactions. Protein J. 27, 253-257 (2008)
Shunsuke Tomita, Hiroyuki Hamada, Yukio Nagasaki, and Kentaro Shiraki. Synergistic effect of polyethylene glycol with arginine on the prevention of heat-induced aggregation of lysozyme. J. Physics 106, 012022 (2008)
Atsushi Hirano, Hiroyuki Hamada, Tatsunori Okubo, Takumi Noguchi, Hiroki Higashibata, and Kentaro Shiraki. Correlation between thermal aggregation and stability of lysozyme with salts described by molar surface tension increment: an exceptional propensity of ammonium salts as aggregation suppressor. Protein J. 26, 423-433 (2007)
Tsuneyoshi Matsuoka, Shunsuke Tomita, Hiroyuki Hamada, and Kentaro Shiraki. Amidated amino acids are prominent additives to prevent heat-induced aggregation of lysozyme. J. Biosci. Bioeng. 103, 440-443 (2007)
Hiroyuki Hamada, and Kentaro Shiraki. L-Argininamide improves the oxidative refolding of lysozyme more effectively than L-arginine. J. Biotechnol. vol. 130, 153-60 (2007)
特許、タンパク質のリフォールディング添加剤及びそれを用いたタンパク質の再生方法。特願2006-167386
浜田 寛之, 白木 賢太郎. アミロイド線維を用いた新しいナノ素材. 現代化学. No. 408, 20-26 (2005).
白木 賢太郎、平野 篤、浜田 寛之. タンパク質凝集抑制剤の構造. 生物工学会誌 vol. 84, No. 10, 395-397 (2006)
修士（工学）、2008年3月
学士（工学）、2006年3月
Sumon Ganguli, Keitaro Yoshimoto, Shunsuke Tomita, Hiroshi Sakuma, Tsuneyoshi Matsuoka, Kentaro Shiraki, and Yukio Nagasaki. Regulation of Lysozyme Activity Based on Thermotolerant Protein/Smart Polymer Complex Formation. J. Am. Chem. Soc., 131, 6549-6553 (2009)
Tsuneyoshi Matsuoka, Hiroyuki Hamada, Koji Matsumoto, and Kentaro Shiraki. Indispensable Structure of Solution Additives to Prevent Inactivation of Lysozyme during Heating and Refolding. Biotechnol. Prog. 25, 1515-1524 (2009)
蛋白質アーカイブ#035「タンパク質リフォールディングの標準的な方法」浜田寛之、松岡常吉. 公開日 2008/6/9
Tsuneyoshi Matsuoka, Shunsuke Tomita, Hiroyuki Hamada, and Kentaro Shiraki. Amidated Amino Acids Are Prominent Additives to Prevent Heat-Induced Aggregation of Lysozyme. J. Biosci. Biochem. vol. 103, 440-443 (2007)
特許、蛋白質の安定化のための温度応答性ポリマーの使用。特願2008-56460
修士（工学）、2008年3月
学士（工学）、2006年3月
Tsuneyoshi Matsuoka, Hiroyuki Hamada, Koji Matsumoto, and Kentaro Shiraki. Indispensable Structure of Solution Additives to Prevent Inactivation of Lysozyme during Heating and Refolding. Biotechnol. Prog. 25, 1515-1524 (2009)
学士（工学）、2007年3月