High-Performance Liquid Chromatography (HPLC)- Laser-Induced Fluorescence Technique for Clinical Applications
High-Performance Liquid Chromatography (HPLC)- Laser-Induced Fluorescence Technique for Clinical Applications
HPLC is a separation technique in combination with various types of detection methods, used to separate, purify, and quantify the individual components of a mixture. HPLC in combination with Laser-Induced Fluorescence (LIF) and LED-Induced fluorescence detection (LED-IF) system is being used for the analysis of clinical samples (serum, tears, saliva, cellular sample, tissue homogenate etc.). HPLC-LIF is an established technique for the detection of various biomolecules (proteins, amino acids, and others) due to its high sensitivity (of the order of femtomoles). In the current scenario, LED is gaining attention due to its size, low power consumption, and cost-effectiveness. Our group is working with the HPLC- LED-IF-based system for generating chromatograms of body fluids collected from healthy volunteers and patients with certain diseases (cancer, cardiovascular, eye diseases, endometriosis). The current system has shown sensitivity (of the order of picomoles) and high specificity for the diagnosis of myocardial infarction, dry eye, and glaucoma. We are also trying to optimize the use of the system for the diagnosis of endometriosis and cervical cancer.
Adigal SS, Bhandary SV, Hegde N, Nidheesh VR, John RV, Rizvi A, George SD, Kartha VB, Chidangil S. Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome. RSC Advances. 2023;13(32):22559-68.
John RV, Devasia T, Adigal SS, Lukose J, Kartha VB, Chidangil S. Serum protein profile study of myocardial infarction using a LED induced fluorescence based HPLC system. Journal of Chromatography B. 2023 Feb 15;1217:123616.
John RV, Devasiya T, VR N, Adigal S, Lukose J, Kartha VB, Chidangil S. Cardiovascular biomarkers in body fluids: progress and prospects in optical sensors. Biophysical Reviews. 2022 Aug;14(4):1023-50.
Adigal SS, Rizvi A, Rayaroth NV, John RV, Barik A, Bhandari S, George SD, Lukose J, Kartha VB, Chidangil S. Human tear fluid analysis for clinical applications: progress and prospects. Expert Review of Molecular Diagnostics. 2021 Aug 3;21(8):767-87.