Laser Induced Fluorescence for Tissue samples

The transformation of normal into malignant tissue is accompanied by biochemical alterations at the cellular level. Laser-induced fluorescence (LIF) can reflect those changes either as variations in the fluorescence intensity or as shifts in the fluorescence maxima of bio fluorophores present in tissues. We fabricated fiber optic probes and assembled the fluorescence setup for the detection and screening of various types of cancers. An oral cancer screening device is already developed by the team. The multicenter trial of the device is funded by the DST which will be resuming soon. By analyzing the in-vitro tissue fluorescence spectra we have demonstrated the discrimination of normal and cervicitis tissue from cervical malignancy (cervical cancer). Often physicians confuse cervicitis with malignancy, whereas it is an inflammatory state of the tissue.  

Since different types of renal cancer show various energy metabolism for their survival, LIF can be used as an effective tool for differentiating various renal cancers as well as detecting cancer from normal renal tissue.  We can detect renal cancer (chromophobe renal cell carcinoma) in vitro using the label-free laser-induced fluorescence (LIF) technique. LIF can be extended to in-vivo detection of various cancers and can also be used for surgical demarcation. It can also be extended for the selection of a biopsy site for the histopathologic confirmation of the malignancy. The use of synchronous fluorescence for the various body fluids can give information about the condition of the subject.   Currently working on the detection of endometriosis from blood plasma and peritoneal fluid using synchronous fluorescence spectroscopy.