My educational and research experience is very interdisciplinary. I hold degrees in chemistry, biochemistry, and microbiology. In graduate school, at the U. of Minnesota, I was able to unite my interests, studying bioinorganic chemistry, my thesis work required various skills in biophysics and physical inorganic chemistry, employing advanced techniques in transient kinetics and spectroscopy. My doctoral and postdoctoral research has focused on understanding small molecule activation involved in the oxygen- and nitrogen-cycles. As a Postdoctoral Research Assistant I studied the mechanism of electron transfer and substrate reduction, by the metalloenzyme nitrogenase, and the mechanism of C-C bond formation by a cytochrome P450, CYP121 from Mycobacterium tuberculosis, a potential drug target for tuberculosis antibiotics. I find teaching very rewarding and hope to excite students about science in the world around them, and in the process by which scientific knowledge is discovered.

Education

PhD Chemistry, U. of Minnesota

MS Chemistry, U. of Minnesota

BS Chemistry, Idaho State U.

BS Biochemistry, Idaho State U.

Minor: Microbiology, Idaho State U.

Experience

2019 - Present San Antonio College, Adjunct Professor of Chemistry

2017-18 Senior Scientist, Air Force Drug Testing Lab, Lackland AFB

2016-17 Postdoctoral Research Assistant, Dept. of Chem. U. of Texas, San Antonio

2015-16 High School Science Teacher, Uintah High School

2013-15 Postdoctoral Research Assistant, Dept. of Chem. and Biochem., Utah State U.

Publications

Fielding, A. J.; Kovaleva, E. G.; Farquhar, E. R.; Lipscomb, J. D.; Que, L. Jr., A hyperactive cobalt-substituted extradiol-cleaving catechol dioxygenase, J. Biol. Inorg. Chem., 2011, 16, 341–55.

Fielding, A. J.; Lipscomb, J. D.; Que, L. Jr., Characterization of an O2 adduct of an active cobalt-substituted extradiol-cleaving catechol dioxygenase, J. Am. Chem. Soc., 2012, 134, 796–9.

Huo, L.; Fielding, A. J.; Li, T.; Chen, Y.; Iwaki, H.; Hosler, J. P.; Hasegawa, Y.; Que, L. Jr.; Liu, A., Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase, Biochemistry, 2012, 51, 5811–21.

Williamson N. Oloo, W. N.; Fielding, A. J.; Que, L. Jr., Rate-determining water-assisted O-O bond cleavage of an FeIII-OOH intermediate in a bio-inspired nonheme iron-catalyzed oxidation, J. Am. Chem. Soc., 2013, 135, 6438–41.

Fielding, A. J.; Lipscomb, J. D.; Que, L. Jr., A two-electron-shell game: Intermediates of the extradiol-cleaving dioxygenases, J. Biol. Inorg. Chem., 2014, 19, 491–504.

Davydov, R.; Khadka, N.; Zhi-Yong Yang, Z.-Y.; Fielding, A. J.; Lukoyanov, D.; Dean, D. R.; Seefeldt, L. C.; Hoffman B. M., Exploring electron/proton transfer and conformational changes in the nitrogenase MoFe protein and FeMo-cofactor through cryoreduction/EPR measurements, Israel J. Chem., 2016, 56, 841–51.

Dornevil, K.; Davis, I.;, Fielding, A. J.; Terrell, J. R.; Liu, A., Crosslinking of cYY tyrosines by CYP121 through catalytic shunt pathway and mechanistic implications, J. Biol. Chem., 2016, 292, 13645–57

Fielding, A. J., Dornevil, K., Ma, L., Davis, I., Liu, A., Probing ligand exchange in the P450 enzyme CYP121 from Mycobacterium tuberculosis: Dynamic equilibrium of the distal heme ligand as a function of pH and temperature., J. Am. Chem. Soc., 2017, 139(48), 17484–99.