Mouse Behavior Scoring

Paradigm: Open Field

I measure anxiety by the duration of time spent from their nose/face initially touching the wall or parallel walking to the wall but once the mouse turned its head away from the wall I stop the duration. My primary output measure was time/duration in minutes.

Rat 11.15

0.16- 0.26 (10s) 0.30- 0.49 (19s) 0.55- 1.08 (13s) 1.17- 1.26 (9s)

1.33-1.41 (8s) 1.45- 1.46 (1s) 1.50- 1.52 (2s) 2.03- 2.06 (3s)

2.11- 2.15 (4s) 2.20- 2.33 (13s) 2.42- 3.04 (22s) 3.19- 3.27 (8s)

3.32- 3.36 (4s) 3.43- 3.45 (2s) 3.47- 3.49 (2s) 3.53-3.55 (2s)

4.03-4.08 (5s) 4.21- 4.26 (5s) 4.32- 4.33 (1s) 4.38-4.53(15s)

4.56- 5.00 (4s)

total time= 152 seconds= 2.53 minutes

Rat 11.16

0.12-0.21 (9s) 0.23-0.25 (2s) 0.29-0.33 (4s) 0.41-0.46 (5s)

0.48-0.49 (1s) 0.51-0.57 (6s) 1.01- 1.07 (6s) 1.17- 1.23 (6s)

1.24- 1.34 (10s) 1.40-1.41 (1s) 1.43- 1.44 (1s) 1.56- 2.05 (9s)

2.06- 2.15 (9s) 2.23- 2.42 (19s) 2.55- 2.58 (3s) 3.01- 3.04 (3s)

3.15- 3.18 (3s) 3.20- 3.23 (3s) 3.25- 3.34 (9s) 3.39- 3.40 (1s)

3.41- 3.43 (2s) 3.46- 3.47 (1s) 3.50- 3.53 (3s) 4.27- 4.29 (2s)

4.33- 4.35 (2s) 4.39- 4.41 (2s) 4.44- 4.56 (12s) 4.57- 5.00 (3s)

total time=137 seconds= 2.28 minutes

Rat 11.19

0.15- 0.18 (3s) 0.20- 0.23 (3s) 0.24- 0.31 (7s) 0.32- 0.40 (8s)

0.45-0.52 (7s) 1.00- 1.08 (8s) 1.12-1.13 (1s) 1.16- 1.18 (2s)

1.20-1.23 (3s) 1.35-1.38 (3s) 1.47- 1.52 (5s) 1.54-1.55 (1s)

1.56-2.00 (4s) 2.04- 2.11 (7s) 2.13- 2.15 (2s) 2.17- 2.24 (7s)

2.30- 2.32 (2s) 2.34- 2.36 (2s) 2.39- 2.40 (1s) 2.55- 2.57 (2s)

3.04- 3.12 (8s) 3.16-3.17 (1s) 3.19-3.20 (1s) 3.29-3.30 (1s)

3.40- 3.42 (2s) 3.45- 3.46 (1s) 3.49- 3.50 (1s) 3.52- 3.54 (2s)

3.55- 4.07 (12s) 4.12- 4.13 (1s) 4.19- 4.21 (2s) 4.33- 4.34 (1s)

4.38- 4.39 (1s) 4.45- 4.46 (1s) 4.52- 4.56 (4s) 4.49- 5.00 (1s)

total time= 118 seconds= 1.96 minutes

Rat 11.14

0.10-0.14 (4s) 0.17-0.18 (1s) 0.20-0.21 (1s) 0.24-0.25 (1s)

0.28-0.29 (1s) 0.31-0.33 (2s) 0.35-0.41 (6s) 0.46-0.47 (1s)

1.01-1.02 (1s) 1.04-1.09 (5s) 1.22-1.23 (1s) 1.38-1.40 (2s)

1.45-1.48 (3s) 1.57-2.07 (10s) 2.09-2.14 (5s) 2.24- 2.27 (3s)

2.36-2.42 (6s) 2.51-2.56 (5s) 3.03-3.07 (3s) 3.11-3.12 (1s)

3.21-3.22 (1s) 3.24-3.27 (3s) 3.47-3.54 (3s) 3.58-4.01 (3s)

4.13-4.14 (1s) 4.22-4.23 (1s) 4.25-4.27 (2s) 4.33-4.37 (4s)

4.39-4.42 (3s) 4.45-4.48 (3s)

Total time= 86 seconds= 1.43 minutes