Publication Summaries

The most up to date information on citations is on Google Scholar.

Schobs L, Lu H, Liu X, Turner R, Bai P, Koot RE, Zhou S, Chasmai M (2021) PyKale: Knowledge-Aware Machine Learning from Multiple Sources in Python. arXiv:2106.09756

Patel AV, Turner RD, Rifflet A, Acosta-Martin AE, Nichols A, Awad MM, Lyras D, Boneca IG, Bern M, Collins M, Mesnage S PGfinder, a novel analysis pipeline for the consistent, reproducible and high-resolution structural analysis of bacterial peptidoglycans. bioRxiv doi: 10.1101/2021.06.01.446515

Harding AL, Murdoch C, Danby S, Hasan MZ, Nakanishi H, Furuno T, Hadad S, Turner RD, Colley HE (2021) Determination of Chemical Irritation Potential Using a Defined Gene Signature Set on Tissue-Engineered Human Skin Equivalents. JID Innovations 1 (2), 100011

Pasquina-Lemonche L, Burns J, Turner RD, Kumar S, Tank R, Mullin N, Wilson JS, Chakrabarti B, Bullough PA, Foster SJ and Hobbs JK (2020) The architecture of the Gram-positive bacterial cell wall. Nature 582, 294–297

Paterson TE, Bari A, Bullock AJ, Turner RD, Montalbano G, Fiorilli S, Vitale-Brovarone C, MacNeil S and Shepherd J (2020) Multifunctional copper-containing mesoporous glass nanoparticles as antibacterial and proangiogenic agents for chronic wounds. Frontiers in Bioengineering and Biotechnology, https://doi.org/10.3389/fbioe.2020.00246

Wingham JR, Turner RD, Shepherd J, Majewski C (2020) Micro-CT for analysis of laser sintered micro-composites. Rapid Prototyping Journal 26 (4), 649-657.

Turner RD, Wingham JR (Joint First Author), Paterson TE, Shepherd J, Majewski C (2020) Use of silver-based additives for the development of antibacterial functionality in Laser Sintered polyamide 12 parts. Scientific Reports 10 (1), 1-11

Manton JD, Xiao Y, Turner RD, Christie G and Rees EJ (2018) ELM: super-resolution analysis of wide-field images of fluorescent shell structures. Methods and Applications in Fluorescence 6 (3), 037001

Summary: A super resolution method for determining the size of microscopic "shells" of various types without the need for a very complicated microscope.

Turner RD, Mesnage S, Hobbs JK, Foster SJ (2018) Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology. Nature Communications 9 (1), 1263

Summary: This blog post.

Weihs F, Wacnik K, Turner RD, Culley S, Henriques R and Foster SJ (2018) Heterogeneous localisation of membrane proteins in Staphylococcus aureus. Nature Scientific Reports 8 (1) 3657

Summary: Cell membranes are essential for life and for bacteria to cause infections. Here, we explore the lack of uniformity in the S. aureus membrane.

Lund VA, Wacnik K, Turner RD (Joint First Author), Cotterell BE, Walther CG, Fenn SJ, Grein F, Wollman AJM, Leake MC, Olivier N, Cadby A, Mesnage S, Jones S and Foster SJ (2018) Molecular coordination of Staphylococcus aureus cell division. eLife 7, e32057

Summary: In this manuscript we used localisation microscopy (PALM/STORM) to investigate the distribution of cell wall synthesis and the proteins responsible for it in high detail. We question current models of growth and division in S. aureus which propose discrete distributions of the components involved.

Renzoni AM, Kelley WL, Rosato RR, Martinez MP, Roch M, Fatouraei M, Haeusser DP, Margolin W, Fenn S, Turner RD, Foster SJ, and Rosato AE (2017) Molecular bases determining daptomycin resistance-mediated re-sensitization to β-lactams ("see-saw effect") in Methicillin Resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 61 (1), e01634-16 (Citations: 5)

Summary: This study contains microscopic analyses of Staphylococcus aureus treated with antibiotics. We investigated the mechanisms by which bacteria are made sensitive to one antibiotic by another.

Tank R, Turner RD, Cadby A, Foster SJ, Hobbs JK (2016) Towards the STORMForce microscope. Microscopy and Analysis, March 16-18

Summary: This article describes our approach to integrating Atomic Force Microscopy and Single Molecule Localisation Microscopy, with the goal of applying this to study the bacterial cell wall.

Turner RD, Foster SJ, Hobbs JK (2016) Atomic force microscopy analysis of bacterial cell wall peptidoglycan architecture. In: Hong HJ, ed. Bacterial Cell Wall Homeostasis: Methods and Protocols. Springer New York, 3-9 (Citations: 4)

Summary: This book chapter contains detailed instructions for preparing bacterial cell walls (from both Gram negative and Gram positive bacteria) for Atomic Force Microscopy.

Wheeler R, Turner RD (Joint First Author), Bailey RG, Salamaga B, Mesnage S, Mohamad SAS, Hayhurst EJ, Horsburgh M, Hobbs JK, Foster SJ (2015) Bacterial cell enlargement requires control of cell wall stiffness mediated by peptidoglycan hydrolases. MBio 6 (4), e00660-15 (Citations: 10)

Summary: In this article, we demonstrated that polymer chain length in the bacterial cell wall affects cell wall stiffness. Bacteria with long polymer chains, and thus stiff cell walls cannot grow properly. We show that the cell wall needs to be stretchy so that bacteria can grow.

Rosado H, Turner RD, Foster SJ, Taylor PW (2015) Impact of the ß-Lactam resistance modifier (-)-epicatechin gallate on the non-random distribution of phospholipids across the cytoplasmic membrane of Staphylococcus aureus. International Journal of Molecular Sciences 16 (8), 16710-16727 (Citations: 5)

Summary: This study investigated the impact of epicatechin gallate (which can be derived from green tea!) on the organisation of the cell membrane in Staphylococcus aureus.

Bailey RG, Turner RD, Mullin N, Clarke N, Foster SJ, Hobbs JK (2014) The interplay between cell wall mechanical properties and the cell cycle in Staphylococcus aureus. Biophysical Journal 107 (12), 2538-2545 (Citations: 7)

Summary: Here, we showed that different parts of the cell wall of Staphylococcus aureus have different mechanical properties and that these are linked to cell wall polymer organisation and to stage in the cell cycle.

Bottomley AL, Kabli AF, Hurd AF, Turner RD, Garcia-Lara J, Foster SJ (2014) Staphylococcus aureus DivIB is a peptidoglycan-binding protein that is required for a morphological checkpoint in cell division. Molecular Microbiology 94 (5), 1041-1064 (Citations: 11)

Summary: This study determined the function of the gene DivIB.

Cartron M, England SE, Chiriac AI, Josten M, Turner RD, Rauter Y, Hurd AF, Sahl HG, Jones S, Foster SJ (2014) Bactericidal activity of the human skin fatty acid cis-6-hexadecanoic acid on Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 58 (7), 3599-3609 (Citations: 16)

Summary: This study was an investigation of the effects of a fatty acid (naturally present on human skin) on Staphylococcus aureus. This work may underpin development of fatty acid derivatives as antimicrobials.

Turner RD, Vollmer W, Foster SJ (2014) Different walls for rods and balls: the diversity of peptidoglycan. Molecular Microbiology 91 (5), 862-874 (Citations: 67)

Summary: This article reviews work on determining cell wall structure in bacteria (mostly the use of microscopy) and sets some of my work into a historical context.

Turner RD, Hurd A, Cadby A, Hobbs JK, Foster SJ (2013) Cell wall elongation mode in Gram negative bacteria is determined by peptidoglycan architecture. Nature Communications 4, 1496 (Citations: 47)

Summary:

Turner RD, Ratcliffe E, Wheeler R, Hobbs JK, Golestanian R, Foster SJ (2010) Peptidoglycan architecture can specify division planes in Staphylococcus aureus. Nature Communications 1, 26 (Citations: 69)

Summary: Staphylococcus aureus can cause several human diseases, some of them fatal. The drug resistant variant (MRSA) is emblematic of antibiotic resistance and repeatedly makes headlines. Staphylococcus aureus and other bacteria are surrounded by a polymer cell wall, and the assembly of this is the target for some of our best antibiotics. In this study, we used Atomic Force Microscopy and fluorescence microscopy to study the structure and assembly mechanism of the cell wall, yielding new insights into how Staphylococcus aureus grows and divides.

Turner RD, Thomson N, Kirkham J, Devine D (2010) Improvement of the pore trapping method to immobilize vital coccoid bacteria for high resolution AFM: A study of Staphylococcus aureus. Journal of Microscopy 238, 102-110 (Citations: 21)

Summary: Atomic Force Microscopy works by using a very small probe attached to a soft spring - this is used to figuratively “feel” all over a surface resulting in 3D map. As the probe scans over the surface it applies force to the sample being imaged, which may cause it to dislodge. In this study, we developed an improved method for anchoring bacteria such that they were stable for imaging. This allowed us to obtain high resolution videos of bacterial cell division.

Turner RD, Kirkham J, Devine D, Thomson N (2009). Second harmonic atomic force microscopy of living Staphylococcus aureus bacteria. Applied Physics Letters 94, 043901 (Citations: 32)