Ritvik Gupta

ritvikiiserm@gmail.com| Ritvik.gupta@mpi-bn.mpg.de | +91 9023667947

Max Planck Institute for Heart and Lung Research, Ludwigstraße 43, 61231 Bad Nauheim, Germany

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PROFILE

Interdisciplinary researcher with skills and experience in RNA biology, Protein engineering/Biochemistry, and Molecular biology
Self-motivated, problem-solving, and adaptable with excellent communication skills. Enthusiastic about all domains of science.

LANGUAGES

English | Hindi | Punjabi | German A1 |

EDUCATION

BS-MS (Bachelor of Science-Master of Science)

Indian Institute of Science Education and Research, Mohali

BS-MS dual degree program aims to integrate the conventional bachelor's and master's programs into a more holistic science education experience, bringing together the biological, chemical, mathematical, physical, and earth sciences. I majored in Biology and minored in Science and Society Studies.

PUBLICATIONS

A compositional view comparing modern biological condensates and primitive phase-separated compartments

Selene M.C. Cannelli, Ritvik Gupta, Tan Nguyen, Arunava Poddar, Srishti Sharma, Prachiti V. Vithole, Tony Z.

Liquid-liquid phase separation (LLPS) is a process that often occurs due to binding between oppositely charged biopolymers, and has gained increasing attention recently due to their ubiquity in biological systems and ability to direct essential cellular processes.

RESEARCH EXPERIENCE

BLUE MARBLE SPACE INSTITUTE OF SCIENCE (BMSIS)| Research Associate

Literature review comparing the similarities and differences of primitive (prebiotic) and modern (biological) phase separation systems in hopes of discovering more about the potential evolution from primitive membrane-less compartments to modern membrane-less organelles and preparing the review paper, which is published in the journal Peptide Science.

RETINAL REGENERATION IN ZEBRAFISH| Master's thesis student

To understand the molecular mechanisms underlying retina regeneration using the zebrafish model. Knocking out genes such as rnf2 and tuba using CRISPR Cas9 and comparing the expression of these mutant fishes with control (non-mutated fishes).

MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH | Master's thesis student

Relevance of Transcriptional Adaptation in human health and disease. I worked on the therapeutic significance of Transcriptional Adaptation in conditions such as Thalassemia and Sickle cell anemia; a transgenic approach was used to target the Human Hemoglobin gene. Apart from the HBB gene, I also worked on the Pannexin 1 gene to observe the phenomena of Transcriptional Adaptation in the Pannexin gene family in HEK cells. CRISPRCas9 was used to knockout Pannexin 1 gene (56.7 Kb).

INTERNATIONAL GENETICALLY ENGINEERED MACHINE (iGEM) | Experimental team leader

I, along with my team, prepared a diagnostic kit for the early detection of oral squamous cell carcinoma through salivary biomarkers. In this project, a prototype for detecting OSCC (Oral Squamous Cell Carcinoma) in an Indian cohort by detecting the presence of abnormally regulated protein bio- markers in the suspect's saliva.

MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH Summer Intern (Vitually)

Identifying possible new models for Transcriptional adaptation/genetic compensation in humans and mice. After completion, the work was presented in front of the lab.

PROTEIN ENGINEERING AND STRUCTURAL BIOCHEMISTRY (IISERM)| Intern

Analyzing phase separation behavior of HU DNA ligases. Extraction and Purification of proteins from Thermus thermophilus bacterial species and trying to study their phase separation behavior.

SYNTHETIC ORGANIC CHEMISTRY (IISERM)| Summer Intern

I worked on moisture-sensitive and organic-catalyzed reactions. I utilized continuous flow technology i.e. used a micro-reactor to carry out certain reactions. I incorporated the use of several chromatography techniques.

SKILLS

RNA and Cell Biology

RNA isolation, SPRI cleanup, cDNA synthesis, qPCR, handling and working with HEK, HUDEP1, HUDEP2, and HAP1 cells, gDNA isolation, and Transfection, CRISPR Cas9, Oligo annealing, and cloning guides into backbone vector, Designing guides for CRISPR Cas9 [tools such as cctop, CRISPR scan, ape].

Molecular Biology

Cloning (digestion, ligation, transformation), PCR, preparing competent cells, mini prep, midi prep, agarose, and SDS gel electrophoresis.

Biochemistry

Protein extraction and purification and all techniques related to it.

Basic Bio-informatics

Primer designing(PCR, qPCR), Sanger Sequencing, Pymol, GraphPad Prism, Microsoft Word, Excel, and PowerPoint, Image J.

WORKSHOPS/CONFERENCES

BluScicon (Blue Marble Space Science Conversations)

Gave a talk/Presented my work on "comparative studies between modern and Primitive Phase separated systems" at BluSciCon.

EMBO EMBL| Cellular Mechanisms Driven by Phase Separation: Attended the EMBO|EMBL: Cellular Mechanisms Driven by Phase separation conference and did the role of event reporter at the conference.
SPARC-INDO-US Immunology Workshop - Indian Institute of Technology, Ropar

Various techniques related to Immunobiology were discussed, followed by a series of talks.

CERTIFICATES

ACHIEVMENTS

William G. Kerckhoff-Stiftung : William G Kerckhoff Scholarship, at Max Planck Institute for Heart and Lung, Germany.
Experimental team leader iGem. One of the 8 students selected to represent IISER Mohali in iGEM 2021, an independent international competition founded by the Massachusetts Institute of Technology for the advancement of synthetic biology.
Winner of Impact Grant from the Frederick Gardner Cottrell Foundation for iGEM 2021; one of the top 90 teams worldwide to secure this grant.
Academic Excellence Award (Achieved 10/10 SPI)
Awarded level A recognition by NCC(National Cadet Corps).

EXTRA-CURRICULAR ACTIVITIES

EMBO|EMBL

· Attendee/Event reporter at EMBO | EMBL: Cellular mechanisms driven by Phase separation. Blog on the event: blog/embo/ritvik

Science Blogging

Website: https://thesigmabiol.wixsite.com/biology

Instagram Handle: https://instagram.com/sigmabiol?utm_medium=copy_link

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