Publications
27. Rose, S. M.; Silky Bedi; Sabyasachi Rakshit*; Sharmistha Sinha*(2024, Just Accepted); Nanoscale, substrate-induced Phase Transition within Liquid Condensates Reverses Catalytic Activity of Nanoparticles.
26. Nisha Arora, Jagadish P. Hazra, Sandip Roy, Gaurav K. Bhati, Sarika Gupta, K. P. Yogendran, Abhishek Chaudhuri, Amin Sagar, Sabyasachi Rakshit. (2024); Nature Commun. Emergence of slip-ideal-slip behavior in tip-links serve as force filters of sound in hearing.
25. Pritam Saha, Vishavdeep Vashisht, Ojas Singh, Gaurav Bhati, Surbhi Garg, Sabyasachi Rakshit. (2024); Research Square . Heterogeneity in conformational state space enhances the force-tolerance of mechanosensory proteins. https://doi.org/10.21203/rs.3.rs-2677234/v1
24. Veerpal Kaur; Sanat Ghosh; Tripta Bhatia; Sabyasachi Rakshit(2023); Biochemistry. Redefining the structure of tip-links in hair-cells.
23. Veerpal Kaur; Surbhi Garg*; Sabyasachi Rakshit*(2023); Chem Commun., Polyprotein synthesis: a journey from the traditional pre-translational method to modern post-translational approaches for single-molecule force spectroscopy. https://pubs.rsc.org/en/content/articlelanding/2023/CC/D3CC01756G
22. Silky Bedi, Gaurav Kumar, S. M. Rose, Sabyasachi Rakshit* and Sharmistha Sinha. (2022); Chem Comm. Barrier-free liquid condensates of nanocatalysts as effective concentrators of catalysis. https://doi.org/10.1039/D2CC03111F
21. Veerpal Kaur, Surbji Garg, Sabyasachi Rakshit. (2022); Soft Matter. Instantaneous splice and excise of inteins to synthesize polyproteins on a substrate with tunable linkers,18, 602-608 .
20. Sai Srinivas*, Gayathri S. Singaraju*, Veerpal Kaur*, Sayan Das, Sabyasachi Rakshit (2023)Commun. Biol. Transient interactions drive the lateral clustering of Cadherin-23 on membrane (BIORXIV/2022/475209).
19. Nisha Arora, Jagadish Prasad Hazra*, Sabyasachi Rakshit* (Commun. Biol.); Anisotropy in mechanical unfolding of protein upon partner-assisted pulling and handle-assisted pulling (https://doi.org/10.1038/s42003-021-02445-y).
18. Tanuja Joshi, Surbhi Garg, Alejandro Estaña, Juan Cortes, Pau Bernadó, Sayan Das, Anjana R. Kammath, Amin Sagar*, Sabyasachi Rakshit* (BBRC, 2021); Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins.
Highlights
>Random-coil linkers (-polyGly here) maintain the domain independence in polyproteins of Immunoglobulin repeats.
>Linkers (-RS here) that facilitate interdomain contacts may alter the energy landscape of individual domains cooperatively.
>A simple tripeptide-based modeling approach captures the inter-domain orientations across the IDLs.
17. Surbhi Garg1, Amin Sagar1, Gayathri S. Singaraju1, Rahul Dani, Naimat K. Bari, Athi N. Naganathan*, Sabyasachi Rakshit* (2020); Biochem. J., Weakening of Interaction Networks with Aging in Tip-Link Proteins Induces Hearing Loss., 550, 43-48.
Therapeutic remedy to Age-related hearing-loss (ARHL) still remains myth in absence of biophysical models for aging. We, in search of biophysical model for ARHL, traced a single-point mutation, serine to proline, in tip-link protein that aggressively progresses the hearing-loss at early ages. We report a loss in the circular cross-correlated motions among intradomain β-strands that restore proteins from conformational deformations from sound stimuli with mutation, and may trigger ARHL with normal aging.
16. Ankush Garg, Jagadish Prasad Hazra, Malay Kumar Sannigrahi, Sabyasachi Rakshit*,Sharmistha Sinha* (2019); Biophys. J., Variable Mutations at the p53-R273 Oncogenic Hotspot Position Leads to Altered Properties.
15. Jagadish P. Hazra,Amin Sagar, Nisha Arora, Debadutta Deb, Simerpreet Kaur, and Sabyasachi Rakshit. (2019); BioChem. J. (Open Access) Broken force dispersal network in tip-links by the mutations at the Ca2+ -binding residues induces hearing-Loss. 476 (16): 2411-2425.
Mutations at the Ca2+ binding linker regions alter the force-propagation through the tip-links and promote the force-induced dissociation.
14. G. S. Singaraju, A. Kumar, J. S. Samuel, A. Sagar, J. P. Hazra, M. K. Sannigrahi, R. M. Yennamalli, Ashish and S. Rakshit. (2019); FEBS J.(Open Access) Structural basis of the strong cell‐cell junction formed by cadherin‐23. (BioRchiv; SneakPeak, 10.1111/febs.15141 )
A cartoon representation of cadherin-23 (CDH23) proteins that interact from opposing cell-surfaces and mediate the cell-cell junction. To highlight the difference in the interaction conformation from classical cadherins, we also represented the interaction conformation for classical E-cadherin.
13. Malay K. Sannigrahi, Sai Srinivas, Nilesh Deokate and Sabyasachi Rakshit. (2019); Mol. Oncology. Strong Aggregation-propensity of Cadherin-23 inhibit cell migration.
Cadherin-23 is uniformly expressed at the cell-boundaries of various tissues
Cadherin-23 mediated cell-cell junction hinders cancer cell-migration
12. Malay Kumar Sannigrahi, Sai Srinivas and Sabyasachi Rakshit. (2018), Advances in Experimental Medicine and Biology (Springer Books). The prospects of Cadherin-23 as a mediator of homophillic cell-cell adhesion. 1112:99-105.
Cadherin-23, a long non-classical cadherin is uniformly expressed in normal tissues and down-regulated in cancer.
11. Jagadish P. Hazra, Nisha Arora, Amin Sagar, Shwetha Srinivasan, Abhishek Chaudhuri, and Sabyasachi Rakshit. (2018); Biochem. J. (Cover Page) Force-activated catalytic pathway accelerates bacterial-adhesion against flow. 475 (16): 2611-2620.
Publications from Collaborations
4. S Rani, B Dasgupta, GK Bhati, K Tomar, S Rakshit, S Maiti . (2020), ChemBioChem. , Superior Proton Transfer Catalytic Promiscuity of Cytochrome C in Self‐organized Media.
3. Mandeep Singh,Ana Guzman-Aranguez, Afzal Hussain, Cheerneni S Srinivas, Indu P Kaur. (2019), Nanomedicine , Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics.
2. Naimat K. Bari, G. Kumar, A. Bhatt, J.P.Hazra, A. Garg, Md. Ehsan Ali and Sharmistha Sinha. (2018) , ACS catal. (Just Accepted) Nanoparticle fabrication on Bacterial Microcompartment surface for the development of hybrid enzyme-inorganic catalyst.
1. Naimat K.Bari, Shaswat Barua, Ankush Garg, Malay K.Sannigrahi, Sharmistha Sinha. (2018) Cellulose-metallothionein matrix for metal binding. Carb. Pol. 192, 126-134.