Objective:
The project titled "PRODUCTION OF BIODEGRADABLE PLASTIC FROM POLLUTING PLASTIC" was developed for the SynBio Bootcamp 1.0 organized by iGEM Panama, this project seeks to transform the plastic pollutant Polyethylene (PET) into a biodegradable plastic for reuse. Using three constructs: one that degrades PET through the enzyme PETase, MHETase, and Candida antarctica Lipase B (CALB), converting it into terephthalate (tpa); a second that degrades the TPA, converting it into Protocatecuato (PCA) and a third that transforms the PCA into 2-pyrone-4,6-dicarboxylate (PDC) which, when polymerized, is a fundamental component for the construction of biodegradable polymers. Finally, this design is designed to be implemented at an industrial level where part or all of the PET plastics, which are discarded daily, are reused and thus contribute to reducing pollution in the seas and soils of Panama.
Technologies used in the development of the project:
Development tool: Benchling
Project management: Zoom, Google Docs