研究業績

■最近発表した論文

2025

LIPID RICH 1 Modulates Allocation of Carbon between Starch and Triacylglycerol in Arabidopsis Leaves.

Yamaguchi M, Shigenobu S, Yamaguchi K, Higashi Y, Okazaki Y, Saito K, Mishiro-Sato E, Kano K, Sugiyama R, Yamazaki M, Sugano SS, Fukuyoshi S, Ueda H, Hara-Nishimura I, Shimada TL.

J Exp Bot. 2025 Feb 8:eraf048.

Plant-specific tail-anchored coiled-coil protein MAG3 stabilizes Golgi-associated ERESs to facilitate protein exit from the ER.

Takagi J, Takahashi H, Moriya KC, Nagano M, Fukao Y, Ueda H, Tamura K, Shimada T, Hara-Nishimura I.

Commun. Biol. 2025 8, 358.

2024

Quantitative analysis of the root posture of Arabidopsis thaliana mutants with wavy roots.

Yagi H, Hara-Nishimura I, Ueda H.

Quant Plant Biol. 9, e5.

Lipid droplets in Arabidopsis thaliana leaves contain myosin-binding proteins and enzymes associated with furan-containing fatty acid biosynthesis.

Omata Y, Sato R, Mishiro-Sato E, Kano K, Ueda H, Hara-Nishimura I, Shimada TL.

Front Plant Sci. 2024 Mar 1:15:1331479.

ARGONAUTE1-binding Tudor domain proteins function in small interfering RNA production for RNA-directed DNA methylation.

Takei T, Tsukada M, Tamura K, Hara-Nishimura I, Fukao Y, Kurihara Y, Matsui M, Saze H, Tsuzuki M, Watanabe Y, Hamada T.

Plant Physiol. 2024 Mar 6:kiae135.

2023

The Arabidopsis katamari2 Mutant Exhibits a Hypersensitive Seedling Arrest Response at the Phase Transition from Heterotrophic to Autotrophic Growth.

Hosokawa C, Yagi H, Segami S, Nagano AJ, Koumoto Y, Tamura K, Oka Y, Matsushita T, Shimada T.

Plant Cell Physiol. 2023 Dec 7:pcad156.

Shoot gravitropism and organ straightening cooperate to arrive at a mechanically favorable shape in Arabidopsis.

Tugawa S, Miyake Y, Okamoto K, Toyota M, Yagi H, Morita MT, Hara-Nishimura I, Demura T, Ueda H.

Sci Rep. 2023 July;13:11165.

Pexophagy in plants: a mechanism to remit cells from oxidative damage caused under high-intensity light.

Goto-Yamada S, Oikawa K, Hayashi Y, Mano S, Yamada K, Nishimura M.

Autophagy. 2023 May;19(5):1611-1613.

Stomatal regulators are co-opted for seta development in the astomatous liverwort Marchantia polymorpha.

Moriya KC, Shirakawa M, Loue-Manifel J, Matsuda Y, Lu Y-T, Tamura K, Oka Y, Matsushita T, Hara-Nishimura I, Ingram G, Nishihama R, Goodrich J, Kohchi T, Shimada T.

Nat Plants. 2023 Feb;9(2):302-314.

2022

Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light.

Oikawa K, Goto-Yamada S, Hayashi Y, Takahashi D, Kimori Y, Shibata M, Yoshimoto K, Takemiya A, Kondo M, Hikino K, Kato A, Shimoda K, Ueda H, Uemura M Numata K, Ohsumi Y, Hara-Nishimura I, Mano S, Yamada K, Nishimura M.

Nat Commun. 2022 Dec 5;13(1):7493.

Image-Based Analysis Revealing the Molecular Mechanism of Peroxisome Dynamics in Plants.

Goto-Yamada S, Oikawa K, Yamato KT, Kanai M, Hikino K, Nishimura M, Mano S.

Front Cell Dev Biol. 2022 May 3;10:883491.

Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.

Hu S, Li B, Wu F, Zhu D, Zouhar J, Gao C, Shimada T, Rojo E, Hara-Nishimura I, Jiang L, Shen J.

Proc Natl Acad Sci U S A. 2022 May 17;119(20):e2200492119.

Aerial (+)-borneol modulates root morphology, auxin signaliing and meristemtic activity in Arabidopsis roots.

Fukuda K, Uefune M, Fukaki H, Yamauchi Y, Hara-Nishimura I, Ozawa R, Matsui K, Sugimoto K, Okada K, Imai R, Takahashi K, Enami S, Wurst R, Takabayashi J.

Biol Lett. 2022 May;18(5):20210629.

2021

Spatiotemporal relationship between auxin dynamics and hydathode development in Arabidopsis leaf teeth.

Yagi H, Tamura K, Matsushita T, Shimada T.

Plant Sign Behav. 2021 Oct 25;1989216.

A Rich and Bountiful Harvest: Key Discoveries in Plant Cell Biology.

Cheung AY, Cosgrove DJ, Hara-Nishimura I, Jürgens G, Lloyd C, Robinson DG, Staehelin LA, Weijers D.

Plant Cell. 2021 Sep 15;koab234 Online ahead of print.

Galactoglucomannan structure of Arabidopsis seed-coad mucilage in GDP-mannose synthesis impaired mutants.

Nishigaki N, Yoshimi Y, Kuki H, Kunieda T, Hara-Nishimura I, Tsumuraya Y, Takahashi D, Dupree P, Kotake T.

Physiol Plant. 2021 Nov;173(3):1244-1252.

Regulation and physiological significance of the nuclear shape in plants.

Goto C, Hara-Nishimura I, Tamura K.

Front Plant Sci. 2021 Jun 10;12:673905.

In vitro assembly of nuclear envelope in tobacco cultured cells.

Tamura K, Ueda H, Hara-Nishimura I.

Nucleus. 2021 Dec 12(1):82-89.

Excess sterol accumulation affects seed morphology and physiology in Arabidopsis thaliana.

Shimada TL, Ueda T, Hara-Nishimura I.

Plant Signal Behav. 2021 Apr 3;16(4):1872217.

The Arabidopsis transcription factor NAI1 activates the NAI2 promoter by binding to the G-box motifs.

Sarkar S, Stefanik N, Kunieda T, Hara-Nishimura I, Yamada K.

Plant Signal Behav. 2021 Feb 1;16(2):1846928.

2020

Mechanical measurement of gravitropic bending force in pea sprouts.

Nakaso Y, Arimoto S, Kawaguchi K, Muto T, Ueda H.

Plant Biotechnol (Tokyo). 2020 Dec 25;37(4):475-480.

Estimating the flexural rigidity of Arabidopsis inflorescence stems: Free-vibration test vs. three-point bending test.

Nakata MT, Nakao M, Denda A, Onoda Y, Ueda H, Demura T.

Plant Biotechnol (Tokyo). 2020 Dec 25;37(4):471-474.

Subnuclear gene positioning through lamina association affects copper tolerance.

Sakamoto Y, Sato M, Sato Y, Harada A, Suzuki T, Goto C, Tamura K, Toyooka K, Kimura H, Ohkawa Y, Hara-Nishimura I, Takagi S, Matsunaga S.

Nat Commun. 2020 Nov 20;11(1):5914.

Higher Stomatal Density Improves Photosynthetic Induction and Biomass Production in Arabidopsis Under Fluctuating Light.

Sakoda K, Yamori W, Shimada T, Sugano SS, Hara-Nishimura I, Tanaka Y.

Front Plant Sci. 2020 Oct 21;11:589603.

The nuclear envelope protein KAKU4 determines the migration order of the vegetative nucleus and sperm cells in pollen tubes.

Goto C, Tamura K, Nishimaki S, Maruyama D, Hara-Nishimura I.

J Exp Bot. 2020 Oct 22;71(20):6273-6281.

Characterization of rhizome transcriptome and identification of a rhizomatous ER body in the clonal plant Cardamine leucantha.

Araki KS, Nagano AJ, Nakano RT, Kitazume T, Yamaguchi K, Hara-Nishimura I, Shigenobu S, Kudoh H.

Sci Rep. 2020 Aug 6;10(1):13291.

Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5 -B.

Nagano M, Ueda H, Fukao Y, Kawai-Yamada M, Hara-Nishimura I.

Plant Signal Behav. 2020 Sep 1;15(9):1790196.

Dynamic Capture and Release of Endoplasmic Reticulum Exit Sites by Golgi Stacks in Arabidopsis.

Takagi J, Kimori Y, Shimada T, Hara-Nishimura I.

iScience. 2020 Jul 24;23(7):101265.

Arabidopsis ECHIDNA protein is involved in seed coloration, protein trafficking to vacuoles, and vacuolar biogenesis.

Ichino T, Maeda K, Hara-Nishimura I, Shimada T.

J Exp Bot. 2020 Jul 6;71(14):3999-4009.

Excess sterols disrupt plant cellular activity by inducing stress-responsive gene expression.

Shimada TL, Yamaguchi K, Shigenobu S, Takahashi H, Murase M, Fukuyoshi S, Hara-Nishimura I.

J Plant Res. 2020 May;133(3):383-392.

A space-saving visual screening method, Glycine max FAST, for generating transgenic soybean.

Iwabuchi K, Shimada TL, Yamada T, Hara-Nishimura I.

Plant Signal Behav. 2020;15(2):1722911.

Endoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants.

Yamada K, Goto-Yamada S, Nakazaki A, Kunieda T, Kuwata K, Nagano AJ, Nishimura M, Hara-Nishimura I.

Commun Biol. 2020 Jan 14;3(1):21.

NAI2 and TSA1 Drive Differentiation of Constitutive and Inducible ER Body Formation in Brassicaceae.

Stefanik N, Bizan J, Wilkens A, Tarnawska-Glatt K, Goto-Yamada S, Strzałka K, Nishimura M, Hara-Nishimura I, Yamada K.

Plant Cell Physiol. 2020 Apr 1;61(4):722-734.

Vacuolar processing enzymes in the plant life cycle.

Yamada K, Basak AK, Goto-Yamada S, Tarnawska-Glatt K, Hara-Nishimura I.

New Phytol. 2020 Apr;226(1):21-31.

Arabidopsis FLYING SAUCER 2 Functions Redundantly with FLY1 to Establish Normal Seed Coat Mucilage.

Kunieda T, Hara-Nishimura I, Demura T, Haughn GW.

Plant Cell Physiol. 2020 Feb 1;61(2):308-317.

Regulation of Sugar and Storage Oil Metabolism by Phytochrome during De-etiolation.

Kozuka T, Sawada Y, Imai H, Kanai M, Hirai MY, Mano S, Uemura M, Nishimura M, Kusaba M, Nagatani A.

Plant Physiol. 2020 Feb;182(2):1114-1129.

2019

Sucrose Starvation Induces Microautophagy in Plant Root Cells.

Goto-Yamada S, Oikawa K, Bizan J, Shigenobu S, Yamaguchi K, Mano S, Hayashi M, Ueda H, Hara-Nishimura I, Nishimura M, Yamada K.

Front Plant Sci. 2019 Dec 3;10:1604.

HIGH STEROL ESTER 1 is a key factor in plant sterol homeostasis.

Shimada TL, Shimada T, Okazaki Y, Higashi Y, Saito K, Kuwata K, Oyama K, Kato M, Ueda H, Nakano A, Ueda T, Takano Y, Hara-Nishimura I.

Nat Plants. 2019 Nov;5(11):1154-1166.

AtUBL5 regulates growth and development through pre-mRNA splicing in Arabidopsis thaliana.

Watanabe E, Mano S, Nishimura M, Yamada K.

PLoS One. 2019 Nov 15;14(11):e0224795.

Biogenesis of leaf endoplasmic reticulum body is regulated by both jasmonate-dependent and independent pathways.

Nakazaki A, Yamada K, Kunieda T, Tamura K, Hara-Nishimura I, Shimada T.

Plant Signal Behav. 2019;14(8):1622982.

tRNA wobble modification affects leaf cell development in Arabidopsis thaliana.

Nakai Y, Horiguchi G, Iwabuchi K, Harada A, Nakai M, Hara-Nishimura I, Yano T.

Plant Cell Physiol. 2019 May 10. pii: pcz064.

Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction.

Goto C, Hashizume S, Fukao Y, Hara-Nishimura I, Tamura K.

Nucleus. 2019 Dec;10(1):81-92.

Autophagy controls reactive oxygen species homeostasis in guard cells that is essential for stomatal opening.

Yamauchi S, Mano S, Oikawa K, Hikino K, Teshima KM, Kimori Y, Nishimura M, Shimazaki KI, Takemiya A.

Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):19187-19192.

Re-evaluation of physical interaction between plant peroxisomes and other organelles using live-cell imaging techniques.

Oikawa K, Hayashi M, Hayashi Y, Nishimura M.

J Integr Plant Biol. 2019 Jul;61(7):836-852.

Identification of Periplasmic Root-Cap Mucilage in Developing Columella Cells of Arabidopsis thaliana.

Maeda K, Kunieda T, Tamura K, Hatano K, Hara-Nishimura I, Shimada T.

Plant Cell Physiol. 2019 Jun 1;60(6):1296-1303.

Soybean (Glycine max L.) triacylglycerol lipase GmSDP1 regulates the quality and quantity of seed oil.

Kanai M, Yamada T, Hayashi M, Mano S, Nishimura M.

Sci Rep. 2019 Jun 20;9(1):8924.

Physiological function of photoreceptor UVR8 in UV-B tolerance in the liverwort Marchantia polymorpha.

Kondou Y, Miyagi Y, Morito T, Fujihira K, Miyauchi W, Moriyama A, Terasawa T, Ishida S, Iwabuchi K, Kubo H, Nishihama R, Ishizaki K, Kohchi T.

Planta. 2019 May;249(5):1349-1364.

Effect of mutation of C-terminal and heme binding region of Arabidopsis catalase on the import to peroxisomes.

Fujikawa Y, Suekawa M, Endo S, Fukami Y, Mano S, Nishimura M, Esaka M.

Biosci Biotechnol Biochem. 2019 Feb;83(2):322-325.

A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones.

Poret M, Chandrasekar B, van der Hoorn RAL, Déchaumet S, Bouchereau A, Kim TH, Lee BR, Macquart F, Hara-Nishimura I, Avice JC.

Front Plant Sci. 2019 Feb 4;10:46.

Polar Localization of the Borate Exporter BOR1 Requires AP2-Dependent Endocytosis.

Yoshinari A, Hosokawa T, Amano T, Beier MP, Kunieda T, Shimada T, Hara-Nishimura I, Naito S, Takano J.

Plant Physiol. 2019 Apr;179(4):1569-1580.

Leaf Endoplasmic Reticulum Bodies Identified in Arabidopsis Rosette Leaves Are Involved in Defense against Herbivory.

Nakazaki A, Yamada K, Kunieda T, Sugiyama R, Hirai MY, Tamura K, Hara-Nishimura I, Shimada T.

Plant Physiol. 2019 Apr;179(4):1515-1524.

How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening.

Ueda H, Hara-Nishimura I.

Methods Mol Biol. 2019;1924:215-221.

A Golgi-Released Subpopulation of the Trans-Golgi Network Mediates Protein Secretion in Arabidopsis.

Uemura T, Nakano RT, Takagi J, Wang Y, Kramer K, Finkemeier I, Nakagami H, Tsuda K, Ueda T, Schulze-Lefert P, Nakano A.

Plant Physiol. 2019 Feb;179(2):519-532.

ANGUSTIFOLIA Regulates Actin Filament Alignment for Nuclear Positioning in Leaves.

Iwabuchi K, Ohnishi H, Tamura K, Fukao Y, Furuya T, Hattori K, Tsukaya H, Hara-Nishimura I.

Plant Physiol. 2019 Jan;179(1):233-247.

2018

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.

Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T.

PLoS One. 2018 Oct 31;13(10):e0205117.

Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium.

Inoue S, Morita R, Kuwata K, Kunieda T, Ueda H, Hara-Nishimura I, Minami Y.

Plant Physiol Biochem. 2018 Nov;132:138-144.

Synaptotagmin-Associated Endoplasmic Reticulum-Plasma Membrane Contact Sites Are Localized to Immobile ER Tubules.

Ishikawa K, Tamura K, Ueda H, Ito Y, Nakano A, Hara-Nishimura I, Shimada T.

Plant Physiol. 2018 Oct;178(2):641-653.

The AP-1 Complex is Required for Proper Mucilage Formation in Arabidopsis Seeds.

Shimada T, Kunieda T, Sumi S, Koumoto Y, Tamura K, Hatano K, Ueda H, Hara-Nishimura I.

Plant Cell Physiol. 2018 Nov 1;59(11):2331-2338.

Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha.

Mano S, Nishihama R, Ishida S, Hikino K, Kondo M, Nishimura M, Yamato KT, Kohchi T, Nakagawa T.

PLoS One. 2018 Oct 4;13(10):e0204964.

Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family.

Takenaka Y, Kato K, Ogawa-Ohnishi M, Tsuruhama K, Kajiura H, Yagyu K, Takeda A, Takeda Y, Kunieda T, Hara-Nishimura I, Kuroha T, Nishitani K, Matsubayashi Y, Ishimizu T.

Nat Plants. 2018 Sep;4(9):669-676.

Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells.

Goto-Yamada S, Hikino K, Nishimura M, Nakagawa T, Mano S.

Methods Mol Biol. 2018;1794:245-258.

Stress granule formation is induced by a threshold temperature rather than a temperature difference in Arabidopsis.

Hamada T, Yako M, Minegishi M, Sato M, Kamei Y, Yanagawa Y, Toyooka K, Watanabe Y, Hara-Nishimura I.

J Cell Sci. 2018 Aug 20;131(16). pii: jcs216051.

Endoplasmic Reticulum (ER) Membrane Proteins (LUNAPARKs) are Required for Proper Configuration of the Cortical ER Network in Plant Cells.

Ueda H, Ohta N, Kimori Y, Uchida T, Shimada T, Tamura K, Hara-Nishimura I.

Plant Cell Physiol. 2018 Oct 1;59(10):1931-1941.

Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana.

Takagi J, Uemura T.

Methods Mol Biol. 2018;1789:155-165.

GREEN FLUORESCENT SEED, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds.

Shimada T, Fuji K, Ichino T, Teh OK, Koumoto Y, Hara-Nishimura I.

Methods Mol Biol. 2018;1789:1-7.

The Multifaceted Roles of Plant Vacuoles.

Martinoia E, Mimura T, Hara-Nishimura I, Shiratake K.

Plant Cell Physiol. 2018 Jul 1;59(7):1285-1287.

Specialized Vacuoles of Myrosin Cells: Chemical Defense Strategy in Brassicales Plants.

Shirakawa M, Hara-Nishimura I.

Plant Cell Physiol. 2018 Jul 1;59(7):1309-1316.

Plant Vacuoles.

Shimada T, Takagi J, Ichino T, Shirakawa M, Hara-Nishimura I.

Annu Rev Plant Biol. 2018 Apr 29;69:123-145.

Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants.

Hatsugai N, Hara-Nishimura I.

Methods Mol Biol. 2018;1743:163-171.

Involvement of Adapter Protein Complex 4 in Hypersensitive Cell Death Induced by Avirulent Bacteria.

Hatsugai N, Nakatsuji A, Unten O, Ogasawara K, Kondo M, Nishimura M, Shimada T, Katagiri F, Hara-Nishimura I.

Plant Physiol. 2018 Feb;176(2):1824-1834.

Membrane Dynamics and Multiple Functions of Oil Bodies in Seeds and Leaves.

Shimada TL, Hayashi M, Hara-Nishimura I.

Plant Physiol. 2018 Jan;176(1):199-207.

2017

HSP90 stabilizes auxin receptor TIR1 and ensures plasticity of auxin responses.

Watanabe E, Mano S, Hara-Nishimura I, Nishimura M, Yamada K.

Plant Signal Behav. 2017 May 4;12(5):e1311439.

Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots.

Wang S, Yoshinari A, Shimada T, Hara-Nishimura I, Mitani-Ueno N, Feng Ma J, Naito S, Takano J.

Plant Cell. 2017 Apr;29(4):824-842.

The chemical compound bubblin induces stomatal mispatterning in Arabidopsis by disrupting the intrinsic polarity of stomatal lineage cells.

Sakai Y, Sugano SS, Kawase T, Shirakawa M, Imai Y, Kawamoto Y, Sugiyama H, Nakagawa T, Hara-Nishimura I, Shimada T.

Development. 2017 Feb 1;144(3):499-506.

Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana.

Tamura K, Fukao Y, Hatsugai N, Katagiri F, Hara-Nishimura I.

Nucleus. 2017 May 4;8(3):301-311.

Isolation of Protein Storage Vacuoles and Their Membranes.

Shimada T, Hara-Nishimura I.

Methods Mol Biol. 2017;1511:163-168.

PYK10 myrosinase reveals a functional coordination between endoplasmic reticulum bodies and glucosinolates in Arabidopsis thaliana.

Nakano RT, Piślewska-Bednarek M, Yamada K, Edger PP, Miyahara M, Kondo M, Böttcher C, Mori M, Nishimura M, Schulze-Lefert P, Hara-Nishimura I, Bednarek P.

Plant J. 2017 Jan;89(2):204-220.