Microbial symbionts can strongly influence insect development, survival, physiology, and ecological interactions, but these effects depend on both the identity of the microbial partner and the environment in which the interaction occurs. Our lab studies how variation among microbial symbionts shapes insect performance under environmental stress.
Much of our research focuses on leaf-footed insect family (Coreidae) and their environmentally acquired bacterial symbionts, Caballeronia. Because both the insects and their symbionts can be experimentally manipulated, these associations provide a tractable system for connecting microbial traits with consequences for host fitness. We combine field collections, insect rearing and experimental assays, microbiology, and genomic analyses to investigate how hosts and their microbial partners respond to environmental variation.
Environmental conditions can determine whether a microbial partner is beneficial, neutral, or harmful to its host. We investigate how insects and their microbial symbionts respond to thermal stress, including sustained high temperatures and short-term heat events. By experimentally pairing insects with different Caballeronia strains, we can test whether variation in symbiont thermal tolerance predicts differences in host performance.
Microbial strains that perform differently under environmental stress may differ substantially in their genomic features. We use comparative genomics alongside laboratory experiments to investigate the genetic basis of variation among insect-associated Caballeronia. By connecting genomic differences among strains with traits such as thermal performance, growth, and host-associated fitness effects, we aim to identify microbial characteristics that contribute to successful symbiosis under different environmental conditions and across geographic ranges.
Microbial symbionts can alter how insects interact with other organisms. We are interested in whether beneficial symbionts influence interactions between insects and pathogens, including the plant pathogen Serratia ureilytica. Future work will examine whether variation in host–symbiont partnerships alters pathogen persistence, infection dynamics, or transmission.
Collect insects and environmentally acquired symbionts across hosts, populations, and environmental conditions.
Manipulate host–symbiont combinations and environmental conditions to measure development, survival, and other fitness-related traits.
Culture microbial symbionts and characterize traits such as growth and thermal performance.
Compare microbial genomes and other molecular datasets to identify genetic differences associated with phenotypic variation.