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Improved method for the assessment of DNA damage (The Comet Assay)
Marcus S. Cooke and Mahsa KarbaschiUS Patent PENDINGImproved method for the assessment of DNA damage (The Comet Assay)
An improved technology with competitive advantages in the field:
Increase in number of cell-containing slides being processed at a time.
Slide carriers facilitate parallel, batch processing of multiple slides reducing manual handling and decreases the risk of damage/loss of fragile cell-containing gels.
The slide carriers make for easy insertion and removal of slides from the electrophoresis tank.
Highly compact design saves bench space.
Enhanced cooling, via ceramic platform and cool pack (no external cooling, such as wet ice, needed).
Multiple experiments in a single run.
50 slides can be run in 20 minutes.
Devices and methods for high-throughput assay
20 Feb 2018Marcus S. Cooke, Pratikkumar Shah, Mahsa Karbaschi, Shekar BhansaliUS Patent 9,897,572Devices and methods for high-throughput assay
Abstract
Apparatuses and associated methods for manipulating an assembly of glass slides employed in cellular assay processes are provided. Each apparatus can accommodate at least one removable rack of slides to undergo electrophoresis in a comet assay. The slides can remain in the same apparatus while being subjected to a sequence of fluid staining and washing with temperature control, advantageously shortening the amount of time required for processing the slides by keeping them in the same workstation for the entire duration of the assay.
High throughput approach for processing slides for all variations of the single cell gel electrophoresis assay
6 August 2019Marcus S. Cooke and Mahsa KarbaschiUS Patent 10,401,323High throughput approach for processing slides for all variations of the single cell gel electrophoresis assay
3 September 2019Marcus S. Cooke and Masha KarbaschiUK Patent GB2531983
Abstract
This invention relates to a method of performing electrophoretic analysis on an substrate comprising: providing a substrate on a surface of at least one supporting member; and performing electrophoresis on the substrate by submerging the or each supporting member in an electrophoresis tank having a base and side walls enclosing a volume containing an electrophoresis buffer and inducing an electric current between ends thereof; wherein the or each supporting member is aligned within the tank such that each surface upon which the substrate is located is nonparallel with respect to the base of the electrophoresis tank and is substantially parallel with the direction of the electric current.
Novel chilling apparatus
23 June 2020Mahsa Karbaschi and Marcus S. CookeUS Patent 10,690,574Novel chilling apparatus
Abstract
The subject invention provides an apparatus for cooling and/or freezing samples. In an exemplary embodiment, the apparatus is a stackable chilling plate used in a comet assay. In specific embodiments, the chilling plate can accommodate glass slides deposited with an agarose gel suspension, wherein the gel is cured by a refrigerant disposed underneath a thermally-conductive top plate. Advantageously, the cooling/freezing apparatus provided herein can easily accommodate the placement of multiple cellular sample slides in a compact configuration.
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