Adductomics Core

Services Offered

Consultation

• Experimental design

• Labeling approaches for targeted and untargeted analysis

• Database searching (Stanstrup doi: 10.3389/fchem.2022.908572)

Primary Sample Processing Approaches

• urine two-phase SPE

• serum two-phase SPE

• tissue DNA extraction and hydrolysis

• cell DNA extraction and hydrolysis

Major Equipment and Instrumentation

• Hybrid Quadrupole-Orbitrap Mass Spectrometer (IQX, Thermo) in-line with HPLC (Vanquish Horizon, Thermo)

Software Analysis

• FeatureHunter ( doi: 10.1021/acs.est.3c04674 )

• MetaboAnalyst ( https://www.metaboanalyst.ca/ )

• MSConvert

• mzMine

Targeted Analysis

• Isotopically labeled adduct standards used to quantify and identify targets

Untargeted Adductome Characterization

• with characteristic fragmentation patterns identified through FeatureHunter

• additional statistical analysis through Metaboanalyst

• putative identification using Stanstrup database

• with or without stable isotope tracking

• with or without relative quantification (i.e., creatinine, SG, dG, etc.)