Publications
2023
The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Ga chaperone and GEF Ric-8A.
Muñoz Reyes D, McClelland D, Arroyo-Urea S, Sánchez-Yepes S, Sabín J, Pérez-Suárez S, Menedez M, Mansilla A, García-Nafría J, Sprang S, Sanchez-Barrena MJ
Elife. 2023 doi: 10.7554/eLife.86151
Gracia P, Cremades N.
Methods Mol Biol. 2023;2551:379-394. doi: 10.1007/978-1-0716-2597-2_25. PMID: 36310216
New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach A, García-Nafría J, Tate CG.
Curr Opin Struct Biol. 2023 Mar 22;80:102574. doi: 10.1016/j.sbi.2023.102574. PMID: 36963163
Molecular Cloning Using In Vivo DNA Assembly.
Arroyo-Urea S, Watson JF, García-Nafría J.
Methods Mol Biol. 2023;2633:33-44. doi: 10.1007/978-1-0716-3004-4_3. PMID: 36853454
Structural insights into promiscuous GPCR-G protein coupling.
Carrión-Antolí A., Mallor-Franco J., Arroyo-Urea S., García-Nafría J.
Progress in Molecular Biology and Translational Science. 2023;195:137-152. doi: 10.1016/bs.pmbts.2022.06.015
2022
Modeling the impact of COVID-19 on future tuberculosis burden.
Tovar M, Aleta A, Sanz J, Moreno Y.
Commun Med (Lond). 2022 Jun 29;2:77. doi: 10.1038/s43856-022-00145-0. eCollection 2022. PMID: 35784445
Alebouyeh S, Cárdenas-Pestana JA, Vazquez L, Prados-Rosales R, Del Portillo P, Sanz J, Menéndez MC, García MJ.
Front Microbiol. 2022 Oct 4;13:956602. doi: 10.3389/fmicb.2022.956602. eCollection 2022. PMID: 36267176
Molecular mechanism for the synchronized electrostatic coacervation and co-aggregation of alpha-synuclein and tau.Gracia P, Polanco D, Tarancón-Díez J, Serra I, Bracci M, Oroz J, Laurents DV, García I, Cremades N.Nat Commun. 2022 Aug 6;13(1):4586. doi: 10.1038/s41467-022-32350-9.
Characterisation of Amyloid Aggregation and Inhibition by Diffusion-Based Single Molecule Fluorescence TechniquesPolanco D, Carrancho A, Gracia P, Cremades NBiophysika. 2022 Nov 21;2(4):506.doi: 10.3390/biophysica2040043
Effects of oligomer toxicity, fibril toxicity and fibril spreading in synucleinopathies.Cascella R, Bigi A, Cremades N, Cecchi C.Cell Mol Life Sci. 2022 Mar 4;79(3):174. doi: 10.1007/s00018-022-04166-9.
The Pathological G51D Mutation in Alpha-Synuclein Oligomers Confers Distinct Structural Attributes and Cellular Toxicity.Xu CK, Castellana-Cruz M, Chen SW, Du Z, Meisl G, Levin A, Mannini B, Itzhaki LS, Knowles TPJ, Dobson CM, Cremades N, Kumita JR.Molecules. 2022 Feb 15;27(4):1293. doi: 10.3390/molecules27041293.
Mechanisms underlying TARP modulation of the GluA1/2-γ8 AMPA receptor.
Herguedas B, Kohegyi BK, Dohrke JN, Watson JF, Zhang D, Ho H, Shaikh SA, Lape R, Krieger JM, Greger IH.Nat Commun. 2022 Feb 8;13(1):734. doi: 10.1038/s41467-022-28404-7.2021
Correa-Macedo W, Fava VM, Orlova M, Cassart P, Olivenstein R, Sanz J, Xu YZ, Dumaine A, Sindeaux RH, Yotova V, Pacis A, Girouard J, Kalsdorf B, Lange C, Routy JP, Barreiro LB, Schurr E.
J Clin Invest. 2021 Nov 15;131(22):e148013. doi: 10.1172/JCI148013.PMID: 34473646
Structure determination of GPCRs: cryo-EM compared with X-ray crystallography.García-Nafría J, Tate CG.Biochem Soc Trans. 2021 Sep 28:BST20210431. doi: 10.1042/BST20210431.
Lipoprotein Metabolism in Familial Hypercholesterolemia.Chemello K, García-Nafría J, Gallo A, Martín C, Lambert G, Blom D.J Lipid Res. 2021 Mar 3:100062. doi: 10.1016/j.jlr.2021.100062.
The role of water in the primary nucleation of protein amyloid aggregation.Camino JD, Gracia P, Cremades N.Biophys Chem. 2021 Feb;269:106520. doi: 10.1016/j.bpc.2020.106520. Epub 2020 Dec 5.
The release of toxic oligomers from α-synuclein fibrils induces dysfunction in neuronal cells.Cascella R, Chen SW, Bigi A, Camino JD, Xu CK, Dobson CM, Chiti F, Cremades N, Cecchi C.Nat Commun. 2021 Mar 22;12(1):1814. doi: 10.1038/s41467-021-21937-3.
α-Helical peptidic scaffolds to target α-synuclein toxic species with nanomolar affinity.Santos J, Gracia P, Navarro S, Peña-Díaz S, Pujols J, Cremades N, Pallarès I, Ventura S.Nat Commun. 2021 Jun 18;12(1):3752. doi: 10.1038/s41467-021-24039-2.
All-or-none amyloid disassembly via chaperone-triggered fibril unzipping favors clearance of α-synuclein toxic species.Franco A, Gracia P, Colom A, Camino JD, Fernández-Higuero JÁ, Orozco N, Dulebo A, Saiz L, Cremades N, Vilar JMG, Prado A, Muga A.Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):e2105548118. doi: 10.1073/pnas.2105548118.
2020
Sanz J, Maurizio PL, Snyder-Mackler N, Simons ND, Voyles T, Kohn J, Michopoulos V, Wilson M, Tung J, Barreiro LB.
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23317-23322. doi: 10.1073/pnas.1820846116. Epub 2019 Oct 14. PMID: 31611381
Khan N, Downey J, Sanz J, Kaufmann E, Blankenhaus B, Pacis A, Pernet E, Ahmed E, Cardoso S, Nijnik A, Mazer B, Sassetti C, Behr MA, Soares MP, Barreiro LB, Divangahi M.
Cell. 2020 Oct 29;183(3):752-770.e22. doi: 10.1016/j.cell.2020.09.062. PMID: 33125891
Self-acetylation at the active site of phosphoenolpyruvate carboxykinase (PCK1) controls enzyme activity.Latorre-Muro P, Baeza J, Hurtado-Guerrero R, Hicks T, Delso I, Hernández-Ruiz C, Velazquez-Campoy A, Lawton AJ, Angulo J, Denu JM, Carrodeguas JA.J Biol Chem. 2020 Dec 17:jbc.RA120.015103. doi: 10.1074/jbc.RA120.015103. Online ahead of print. PMID: 33334880
Multiplicity of α-Synuclein Aggregated Species and Their Possible Roles in Disease.Gracia P, Camino JD, Volpicelli-Daley L, Cremades N.Int J Mol Sci. 2020 Oct 28;21(21):E8043. doi: 10.3390/ijms21218043.
The extent of protein hydration dictates the preference for heterogeneous or homogeneous nucleation generating either parallel or antiparallel β-sheet α-synuclein aggregates.J.D. Camino, P. Gracia, S.W. Chen, J. Sot, I. de la Arada, V. Sebastián, J.L.R. Arrondo, F. Goñi, C.M. Dobson, N. Cremades.Chem. Sci., (2020), DOI: 10.1039/d0sc05297c.
Molecular determinants of β-arrestin coupling to formoterol-bound β1-adrenoceptor.Lee Y., Warne T., Nehme R., Pandey S., Chaturvedi M., Dwivedi-Agnihotri H., Edwards P., García-Nafría J., Leslie A., Shukla AK., Tate CG.Nature. 2020 Jul;583(7818):862-866. doi: 10.1038/s41586-020-2419-1.
Coating and Stabilization of Liposomes by Clathrin-Inspired DNA Self-Assembly.Baumann KN, Piantanida L, García-Nafría J, Sobota D, Voïtchovsky K, Knowles TPJ, Hernández-Ainsa S.ACS Nano. 2020 Feb 25;14(2):2316-2323. doi: 10.1021/acsnano.9b09453. Epub 2020 Feb 5.
Cryo-Electron Microscopy: Moving Beyond X-Ray Crystal Structures for Drug Receptors and Drug Development.García-Nafría J, Tate CG.Annu Rev Pharmacol Toxicol. 2020 Jan 6;60:51-71. doi: 10.1146/annurev-pharmtox-010919-023545. Epub 2019 Jul 26.
Pharmacology and preclinical validation of a novel anticancer compound targeting PEPCK-M.Aragó M, Moreno-Felici J, Abás S, Rodríguez-Arévalo S, Hyroššová P, Figueras A, Viñals F, Pérez B, Loza MI, Brea J, Latorre P, Carrodeguas JA, García-Rovés PM, Galdeano C, Ginex T, Luque FJ, Escolano C, Perales JC.Biomed Pharmacother. 2020 Jan;121:109601. doi: 10.1016/j.biopha.2019.109601.
Trodusquemine displaces protein misfolded oligomers from cell membranes and abrogates their cytotoxicity through a generic mechanism.Limbocker R, Mannini B, Ruggeri FS, Cascella R, Xu CK, Perni M, Chia S, Chen SW, Habchi J, Bigi A, Kreiser RP, Wright AK, Albright JA, Kartanas T, Kumita JR, Cremades N, Zasloff M, Cecchi C, Knowles TPJ, Chiti F, Vendruscolo M, Dobson CM.Commun Biol. 2020 Aug 13;3(1):435. doi: 10.1038/s42003-020-01140-8.
2019
Snyder-Mackler N, Sanz J, Kohn JN, Voyles T, Pique-Regi R, Wilson ME, Barreiro LB, Tung J.
Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1219-1228. doi: 10.1073/pnas.1811758115. Epub 2018 Dec 11. PMID: 30538209
Spotting the old foe-revisiting the case definition for TB.
Houben RMGJ, Esmail H, Emery JC, Joslyn LR, McQuaid CF, Menzies NA, Sanz J, Shrestha S, White RG, Yang C, Cobelens F.
Lancet Respir Med. 2019 Mar;7(3):199-201. doi: 10.1016/S2213-2600(19)30038-4. PMID: 30823971
Harrison GF, Sanz J, Boulais J, Mina MJ, Grenier JC, Leng Y, Dumaine A, Yotova V, Bergey CM, Nsobya SL, Elledge SJ, Schurr E, Quintana-Murci L, Perry GH, Barreiro LB.
Nat Ecol Evol. 2019 Aug;3(8):1253-1264. doi: 10.1038/s41559-019-0947-6. Epub 2019 Jul 29. PMID: 31358949
Tovar M, Arregui S, Marinova D, Martín C, Sanz J, Moreno Y.
Nat Commun. 2019 Nov 29;10(1):5457. doi: 10.1038/s41467-019-13387-9. PMID: 31784512
Efficient and Robust NK-Cell Transduction With Baboon Envelope Pseudotyped Lentivector.
Colamartino ABL, Lemieux W, Bifsha P, Nicoletti S, Chakravarti N, Sanz J, Roméro H, Selleri S, Béland K, Guiot M, Tremblay-Laganière C, Dicaire R, Barreiro L, Lee DA, Verhoeyen E, Haddad E.
Front Immunol. 2019 Dec 16;10:2873. doi: 10.3389/fimmu.2019.02873. eCollection 2019. PMID: 31921138
Design, Synthesis, and Efficacy Testing of Nitroethylene- and 7-Nitrobenzoxadiazol-Based Flavodoxin Inhibitors against Helicobacter pylori Drug-Resistant Clinical Strains and in Helicobacter pylori-Infected Mice.Salillas S, Alías M, Michel V, Mahía A, Lucía A, Rodrigues L, Bueno J, Galano-Frutos JJ, De Reuse H, Velázquez-Campoy A, Carrodeguas JA, Sostres C, Castillo J, Aínsa JA, Díaz-de-Villegas MD, Lanas Á, Touati E, Sancho J.J Med Chem. 2019 Jul 11;62(13):6102-6115. doi: 10.1021/acs.jmedchem.9b00355.
In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning.Watson JF, García-Nafría J.J Biol Chem. 2019 Oct 18;294(42):15271-15281. doi: 10.1074/jbc.REV119.009109.
Cryo-EM structures of GPCRs coupled to Gs, Gi and Go.García-Nafría J, Tate CG.Mol Cell Endocrinol. 2019 May 15;488:1-13. doi: 10.1016/j.mce.2019.02.006.
Architecture of the heteromeric GluA1/2 AMPA receptor in complex with the auxiliary subunit TARP γ8.Herguedas B, Watson JF, Ho H, Cais O, García-Nafría J, Greger IH.Science. 2019 Apr 26;364(6438). pii: eaav9011. doi: 10.1126/science.aav9011.
The Molecular Control of Calcitonin Receptor Signaling.Dal Maso E, Glukhova A, Zhu Y, Garcia-Nafria J, Tate CG, Atanasio S, Reynolds CA, Ramírez-Aportela E, Carazo JM, Hick CA, Furness SGB, Hay DL, Liang YL, Miller LJ, Christopoulos A, Wang MW, Wootten D, Sexton PM.ACS Pharmacol Transl Sci. 2019 Jan 11;2(1):31-51. doi: 10.1021/acsptsci.8b00056.
Druggability Simulations and X-Ray Crystallography Reveal a Ligand-Binding Site in the GluA3 AMPA Receptor N-Terminal Domain.Lee JY, Krieger J, Herguedas B, García-Nafría J, Dutta A, Shaikh SA, Greger IH, Bahar I.Structure. 2019 Feb 5;27(2):241-252.e3. doi: 10.1016/j.str.2018.10.017.
Novel Small Molecules Targeting the Intrinsically Disordered Structural Ensemble of α-Synuclein Protect Against Diverse α-Synuclein Mediated Dysfunctions.Tóth G, Neumann T, Berthet A, Masliah E, Spencer B, Tao J, Jobling MF, Gardai SJ, Bertoncini CW, Cremades N, Bova M, Ballaron S, Chen XH, Mao W, Nguyen P, Tabios MC, Tambe MA, Rochet JC, Junker HD, Schwizer D, Sekul R, Ott I, Anderson JP, Szoke B, Hoffman W, Christodoulou J, Yednock T, Agard DA, Schenk D, McConlogue L.Sci Rep. 2019 Nov 18;9(1):16947. doi: 10.1038/s41598-019-52598-4.
Defining α-synuclein species responsible for Parkinson's disease phenotypes in mice.Froula JM, Castellana-Cruz M, Anabtawi NM, Camino JD, Chen SW, Thrasher DR, Freire J, Yazdi AA, Fleming S, Dobson CM, Kumita JR, Cremades N, Volpicelli-Daley LA.J Biol Chem. 2019 Jul 5;294(27):10392-10406. doi: 10.1074/jbc.RA119.007743. Epub 2019 May 29. Erratum in: J Biol Chem. 2020 Jan 24;295(4):1142.
2018
Projecting social contact matrices to different demographic structures.
Arregui S, Aleta A, Sanz J, Moreno Y.
PLoS Comput Biol. 2018 Dec 7;14(12):e1006638. doi: 10.1371/journal.pcbi.1006638. eCollection 2018 Dec. PMID: 30532206
Arregui S, Iglesias MJ, Samper S, Marinova D, Martin C, Sanz J, Moreno Y.
Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3238-E3245. doi: 10.1073/pnas.1720606115. Epub 2018 Mar 21. PMID: 29563223
BCG Educates Hematopoietic Stem Cells to Generate Protective Innate Immunity against Tuberculosis.
Kaufmann E, Sanz J, Dunn JL, Khan N, Mendonça LE, Pacis A, Tzelepis F, Pernet E, Dumaine A, Grenier JC, Mailhot-Léonard F, Ahmed E, Belle J, Besla R, Mazer B, King IL, Nijnik A, Robbins CS, Barreiro LB, Divangahi M.
Cell. 2018 Jan 11;172(1-2):176-190.e19. doi: 10.1016/j.cell.2017.12.031. PMID: 29328912
Genetic and evolutionary determinants of human population variation in immune responses.
Sanz J, Randolph HE, Barreiro LB.
Curr Opin Genet Dev. 2018 Dec;53:28-35. doi: 10.1016/j.gde.2018.06.009. Epub 2018 Jun 28. PMID: 29960896
Dynamic Acetylation of Phosphoenolpyruvate Carboxykinase Toggles Enzyme Activity between Gluconeogenic and Anaplerotic Reactions.Latorre-Muro P, Baeza J, Armstrong EA, Hurtado-Guerrero R, Corzana F, Wu LE, Sinclair DA, López-Buesa P, Carrodeguas JA, Denu JM.Mol Cell. 2018 Sep 6;71(5):718-732.e9. doi: 10.1016/j.molcel.2018.07.031.
Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go.García-Nafría J, Nehmé R, Edwards PC, Tate CG.Nature. 2018 Jun;558(7711):620-623. doi: 10.1038/s41586-018-0241-9. Epub 2018 Jun 20.
A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits.Almeida-Souza L, Frank RAW, García-Nafría J, Colussi A, Gunawardana N, Johnson CM, Yu M, Howard G, Andrews B, Vallis Y, McMahon HT.Cell. 2018 Jul 12;174(2):325-337.e14. doi: 10.1016/j.cell.2018.05.020. Epub 2018 Jun 7.
Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein.García-Nafría J, Lee Y, Bai X, Carpenter B, Tate CG.Elife. 2018 May 4;7. pii: e35946. doi: 10.7554/eLife.35946.
Multistep Inhibition of α-Synuclein Aggregation and Toxicity in Vitro and in Vivo by Trodusquemine.Perni M, Flagmeier P, Limbocker R, Cascella R, Aprile FA, Galvagnion C, Heller GT, Meisl G, Chen SW, Kumita JR, Challa PK, Kirkegaard JB, Cohen SIA, Mannini B, Barbut D, Nollen EAA, Cecchi C, Cremades N, Knowles TPJ, Chiti F, Zasloff M, Vendruscolo M, Dobson CM.ACS Chem Biol. 2018 Aug 17;13(8):2308-2319. doi: 10.1021/acschembio.8b00466. Epub 2018 Jun 28.
Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments.Chen SW, Cremades N.Methods Mol Biol. 2018;1779:45-60. doi: 10.1007/978-1-4939-7816-8_4.
Best Practices for Generating and Using Alpha-Synuclein Pre-Formed Fibrils to Model Parkinson's Disease in Rodents.Polinski NK, Volpicelli-Daley LA, Sortwell CE, Luk KC, Cremades N, Gottler LM, Froula J, Duffy MF, Lee VMY, Martinez TN, Dave KD.J Parkinsons Dis. 2018;8(2):303-322. doi: 10.3233/JPD-171248.
The contribution of biophysical and structural studies of protein self-assembly to the design of therapeutic strategies for amyloid diseases.Cremades N, Dobson CM.Neurobiol Dis. 2018 Jan;109(Pt B):178-190. doi: 10.1016/j.nbd.2017.07.009. Epub 2017 Jul 12.