Plan to Measure Anxiety in the Open Field: (higher scores means more anxious)

I split the circle into two section: inner and outer circle (inner is the middle part and outer is the perimeter)

Scoring Guidelines:

• time spent in outer circle v. time spent in inner circle

• running +2 v. walking -1

• sitting in one area (longer than 3 seconds) +2

• rearing -1

Video 1: 15

time spent in outer circle: 267 seconds

time spent in inner circle: 40

other three categories added together: -1

Video 2: 16 - this mouse was definitely more anxious than the one from Video 1

time spent in outer circle: 261 seconds

time spent in inner circle: 43 seconds

other three categories added together: +11

Video 3: 14

time spent in outer circle: 265 seconds

time spent in inner circle: 58 seconds

other three categories added together: +37

Video 4: 19

time spent in outer circle: 254 seconds

time spent in inner circle: 55 seconds

other three categories added together: +29