of Pennsylvania. In 2019, Dr. Johnstone was a NephCure® Kidney International-nominated “Nephrotic Syndrome Specialist,” and in both 2018 and 2019 he earned a Faculty Teaching Award from Temple University for his educational work in the Nephrology Fellowship Program, where he served as the program director. Clinical Interests Dr. Johnstone’s clinical interests focus on glomerular and cystic diseases such as Alport syndrome, FSGS, ADPKD, and other conditions. He also treats patients with genetic and hereditary forms of kidney disease, and drug-induced kidney disease. Additionally, Dr. Johnstone has interests in peritoneal dialysis and improving patient safety in the treatment of renal disorders and injuries. Research Focus During Dr. Johnstone’s medical training, his research interests first focused on studies of ion channels in model organisms. His studies then transitioned to examining podocyte biology and the identification of gene variants associated with focal segmental glomerulosclerosis (FSGS). His past research was supported by grants from the National Institutes of Health, the American Heart Association, and NephCure Kidney International. Dr. Johnstone’s research priorities now include clinical studies of FSGS, uremic pruritus, and rare diseases. A sample of Dr. Johnstone’s past published research is included below. • Canetta PA, et al. CureGN Consortium. Health-related Quality of Life in Glomerular Disease. Kidney Int. 2019 May; 95(5): 1209-1224. • Selewski DT, et al. CureGN Consortium. Clinical Characteristics and Treatment Patterns of Children and Adults With IgA Nephropathy or IgA Vasculitis: Findings From the CureGN Study. Kidney Int Rep. 2018 Aug 3; 3(6): 1373-1384. • Trachtman H, et al. DUET Study Group. DUET: A Phase 2 Study Evaluating the Efficacy and Safety of Sparsentan in Patients With FSGS. J Am Soc Nephrol. 2018 Nov; 29(11): 2745-2754. • Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF. Alterations in Nuclear Structure Promote Lupus Autoimmunity in a Mouse Model. Dis Model Mech. 2016 Aug 1; 9(8): 885-897. • Gipson DS, et al. Complete Remission in the Nephrotic Syndrome Study Network. Clin J Am Soc Nephrol. 2016 Jan 7; 11(1): 81-89. • Sorrell SL, Golder ZJ, Johnstone DB, Frankl FEK. Renal Peroxiredoxin 6 Interacts With Anion Exchanger 1 and Plays a Novel Role in pH Homeostasis. Kidney Int. 2016 Jan; 89(1): 105-112. • George B, Verma R, Soofi AA, Garg P, Zhang J, Park TJ, Giardino L, Ryzhova L, Johnstone DB, Wong H, Nihalani D, Salant DJ, Hanks SK, Curran T, Rastaldi MP, Holzman LB. Crk1/2-dependent Signaling Is Necessary for Podocyte Foot Process Spreading in Mouse Models of Glomerular Disease. J Clin Invest. 2012 Feb; 122(2): 674-692. • Sampson MG, et al. Nephrotic Syndrome Study Network. Integrative Genomics Identifies Novel Associations With APOL1 Risk Genotypes in Black NEPTUNE Subjects. J Am Soc Nephrol. 2016 Mar; 27(3): 814-823. • Johnstone DB, Ikizler O, Zhang J, Holzman LB. Background Strain and the Differential Susceptibility of Podocyte-specific Deletion of Myh9 on Murine Models of Experimental Glomerulosclerosis and HIV Nephropathy. PLoS One. 2013 Jul 10; 8(7): e67839. • Johnstone DB, Shegokar V, Nihalani D, Rathore YS, Mallik L, Ashish, Zare V, Ikizler HO, Powar R, Holzman LB. APOL1 Null Alleles From a Rural Village in India Do Not Correlate With Glomerulosclerosis. PLoS One. 2012; 7(12): e51546. • Zhdanova O, Srivastava S, Di L, Li Z, Tchelebi L, Dworkin S, Johnstone DB, Zavadil J, Chong MM, Littman DR, Holzman LB, Barisoni L, Skolnik EY. The Inducible Deletion of Drosha and microRNAs in Mature Podocytes Results in a Collapsing Glomerulopathy. Kidney Int. 2011 Oct; 80(7): 719-730. • Johnstone DB, Zhang J, George B, Léon C, Gachet C, Wong H, Parekh R, Holzman LB. Podocyte-specific Deletion of Myh9 Encoding Nonmuscle Myosin Heavy Chain 2A Predisposes Mice to Glomerulopathy. Mol Cell Biol. 2011 May; 31(10): 2162-2170. • Arif E, Wagner MC, Johnstone DB, Wong HN, George B, Pruthi PA, Lazzara MJ, Nihalani D. Motor Protein Myo1c Is a Podocyte Protein That Facilitates the Transport of Slit Diaphragm Protein Neph1 to the Podocyte Membrane. Mol Cell Biol. 2011 May; 31(10): 2134-2150. • Garg P, Verma R, Nihalani D, Johnstone DB, Holzman LB. Neph1 Cooperates With Nephrin to Transduce a Signal That Induces Actin Polymerization. Mol Cell Biol. 2007 Dec; 27(24): 8698-8712. • Johnstone DB, Holzman LB. Clinical Impact of Research on the Podocyte Slit Diaphragm. Nat Clin Pract Nephrol. 2006 May; 2(5): 271-282. Review. | 5 | RENAL-ELECTROLYTE DIVISION | WINTER 2020 New Study Seeks to Optimize Chronic Kidney Disease Care Manisha Jhamb, MD, MPH, is working on a new National Institutes of Health-funded R01 award (R01DK116957) to improve the care of patients with chronic kidney disease (CKD). Dr. Jhamb is an assistant professor of medicine in the Renal-Electrolyte Division whose clinical research focuses on understanding and improving patient-centered outcomes in patients with chronic kidney disease and end-stage renal disease (ESRD). Dr. Jhamb is particularly interested in testing the effectiveness of clinical interventions to improve patient symptoms and quality-of-life in CKD and ESRD. Her ongoing studies are evaluating technology-based collaborative care interventions on