宋英平¹、謝宏昌²、吳羽婷^{2, *}

¹ 國立台灣大學生物資源暨農學院實驗林管理處對高岳營林區。南投縣信義鄉同富村同和巷11號。

² 國立屏東科技大學森林系。屏東縣內埔鄉老埤村學府路1號。

^* 通訊作者：yutingwu@mail.npust.edu.tw

Pezicula ericae K2 as a new record ectomycorrhizal (ECM) fungal species in Taiwan was isolated from the Pinus taiwanensis mycorrhiza in the field. Pin. taiwanensis seedlings were successfully inoculated with Pez. ericae K2 and transplanted in heavy-metal soil for nearly 6 months. The study had two objectives, to investigate 1. the capacity of heavy metal remediation of Pez. ericae K2 and 2. the effect on the rhizosphere bacterial community composition. Pisolithus tinctorius was used in the study for the comparison. The results showed that plant biomass (dry weight) inoculated with ECM fungi generally significantly higher than the control. Soil K+, Na+, Ca2+, Mg2+ and pH were significantly increased while Zn2+, Cu2+, and As3- were significantly reduced. Pez. ericae K2 absorbed the highest amount of heavy metals. Inoculation of ECM fungi significantly shaped the bacterial community structure in rhizosphere and root tips. Generally, the soil bacterial assemblage was dominated by Acidobacteria, Proteobacteria, and Chloroflexi at phylum level while Actinomycetales, Burkholderiales, Rhizobiales were the dominant orders, among which Burkholderiales and Rhizobiales are important nitrogen-fixing bacteria. The variation of bacterial community structure of root tips was significantly driven by the heavy-metals including Zn2+, Cu2+ and As3- (P < 0.05) while was significantly affected by the environmental factors including soil pH , Mg2+, Ca2+, Na+, K+, Zn2+, Cu2+, As3- (P < 0.05) in the rhizosphere soil. Frankia sp. was only detected in the root tip of Pez. ericae K2 mycorrhiza, which might play an important role in the reduction of soil heavy-metals with Pez. ericae K2.