My work integrates "single-molecule nanoscopy" and "bioanalytical instrumentation" for next-generation biomedical applications, combining "advanced nanomaterials", "super-resolution imaging", "nanopore sensing", and "fluorescence spectroscopy" to uncover molecular-level details of biological systems.
Current Postdoctoral Research: Protein Fingerprinting at the Single-Molecule Level
At the "Meller Lab", Department of Biomedical Engineering, Technion, I am developing "ultrasensitive single-molecule platforms" for "protein sensing and fingerprinting" using "nanopores and fluorescence detection." My current project focuses on creating a "lab-on-a-chip device" that performs "on-chip protein separation, identification, and digital counting"—enabling "real-time biomarker detection" at extremely low concentrations. This technology aims to revolutionize diagnostics by allowing "high-resolution protein analysis" in "clinical samples" such as liquid biopsies, with potential implications in "early disease detection," "personalized medicine," and "proteomics.".
Ph.D. Research: Super-Resolution Imaging with Carbon-Based Nanoprobes
During my doctoral studies with Prof. Chayan Kanti Nandi at the Indian Institute of Technology (IIT) Mandi, I engineered a custom optical TIRF microscope tailored for single-molecule and super-resolution imaging and conducted pioneering work in Stochastic Optical Reconstruction Microscopy (STORM) and Super-Resolution Radial Fluctuation (SRRF).
My research introduced a novel, biocompatible carbon-based fluorescent nanomaterial—termed Fluorescent Carbon Nano Dots (CNDs)—which enabled 3D imaging of thick muscle tissue in C. elegans and opened new possibilities in deep-tissue imaging. I also investigated photophysical artifacts in fluorescent probes and proposed methodologies to enhance blinking-based localization precision.
Technical Expertise
* Single-Molecule Imaging: smFRET, SPT, blinking-based localization, intensity fluctuation analysis
* Superresolution Microscopy: STORM, SRRF, O-TIRF microscopy, custom-built systems
*Spectroscopy & Advanced Imaging: Fluorescence Correlation Spectroscopy (FCS), Fluorescence Lifetime Imaging Microscopy (FLIM)
* Nanomaterials Development: Carbon nanodots, quantum dots, superparamagnetic fluorescent nanoparticles
*Labeling & Targeting: Protein labeling with carbon-based fluorophores for specific molecular targeting