Embedded Files
Publications
2025
29. Min Ju Jo, and Hye Jin Nam* (2025) Protocol to Screen Chemicals to Enhance Homology-Directed Repair in CRISPR-Cas9 gene editing., Star Protocols 6, 103896, September 19, 2025 https://doi.org/10.1016/j.xpro.2025.103896 ( *corresponding author)
Protocol for screening chemicals that enhance HDR efficiency in human cells.
Instructions for rapid identification of HDR-enhancing compounds in a single assay.
Suitable for high-throughput screening using a standard plate reader.
28. Hye Jin Nam†*, Jun Hee Han†, Jihyeon Yu†, Chang Sik Cho, Dongha Kim, Young Eun Kim, Min Ji Kim, Dong Hyun Jo*, and Sangsu Bae* (2025) Autophagy induction enhances homologous recombination-associated CRISPR-Cas9 gene editing., Nucleic Acids Research, Volume 53, Issue 7, 24 April 2025, gkaf258, https://doi.org/10.1093/nar/gkaf258 (†co-first, *co-corresponding author: lead contact)
Autophagy induction significantly enhances homologous recombination (HR)-mediated CRISPR-Cas9 gene editing across multiple genomic loci.
Cas9-interacting cofactors under autophagic conditions were identified as key modulators of HR efficiency.
In vivo autophagy activation boosts precise gene correction and insertion in mouse models, suggesting therapeutic applicability.
언론 보도: 유전자 편집 새길 열었다. '자가포식'으로 효율 최대 3배 높여
https://www.chosun.com/economy/science/2025/05/25/YJV4WQUKOBGU33W5RQQYZ4KBHU/
https://www.dongascience.com/news/view/71867
http://www.fnnews.com/news/202505231352572160
https://www.yna.co.kr/view/AKR20250523103500063
https://www.etnews.com/20250523000117
27. Dongha Kim, Hye Jin Nam*, and Sung Hee Baek* (2025) Ubiquitination of Transcription Factors in Cancer: Unveiling Therapeutic Potential., Molecular Oncology, https://doi.org/10.1002/1878-0261.70033 ( *co-corresponding author)
Graphical Abstract
Graphical Abstract
26. Jeong Woon Jang, Min Ji Kim, Chang Sik Cho, Jun Hee Han, Sangsu Bae, Hongjun Jeon, Dong Hyun Jo, and Hye Jin Nam* (2025) Optimizing HDAC inhibitors to enhance HDR-associated CRISPR-Cas9 gene editing efficiency in vivo and in vitro., Heliyon, https://doi.org/10.1016/j.heliyon.2025.e42971 (*corresponding author)
HDAC inhibitors to enhance HDR-associated CRISPR-Cas9 gene editing efficiency in vivo and in vitro
2024
25. Hong, H.†, Jun, Y.†, Yoon, S.-B., Park, S., Lee, J., Jang, J.W., Nam, H.J.* and Cho, H.* (2024) Manufacturing Uniform Cerebral Organoids for Neurological Disease Modeling and Drug Evaluation., Biomaterials Research, DOI: 10.34133/bmr.0104 (*Co-corresponding author)
Manufacturing Uniform Cerebral Organoids
Uniform cerebral organoids (UCOs) were generated with high reproducibility and morphological consistency by optimizing organoid size and differentiation monitoring.
UCOs recapitulated early human corticogenesis and exhibited functional neural networks with both excitatory and inhibitory responses.
Neurodevelopmental disease models using UCOs revealed drug-reversible neurite outgrowth defects, supporting their utility for therapeutic screening.
2023
24. Kim, D., Nam, H.J.*, Baek, S.H.* (2023) Post-translational Modifications of Lysine-Specific Demethylase 1., Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, https://doi.org/10.1016/j.bbagrm.2023.194968 (*Co-corresponding author)
23. Hong, H., Yoon, S.-B., Park, J.E., Lee, J.I., Kim, H.Y., Nam, H.J., Cho, H. (2023) MeCP2 dysfunction prevents proper BMP signaling and neural progenitor expansion in brain organoid ., Annals of Clinical and Translational Neurology, https://doi.org/10.1002/acn3.51799.
2022
22. Kim, D., Nam, H.J.* (2022) PARP Inhibitors: Clinical Limitations and Recent Attempts to Overcome Them., International Journal of Molecular Sciences 23.15 (2022): 8412. https://doi.org/10.3390/ijms23158412 (*corresponding author): 95회 이상 피인용
21. Takwale,A.D., Kim, E.Y., Jang, Y., Lee, D.H., Kim, S., Choi, Y., Kim, J.H., Lee, D.Y. Kim, Y., Lee, S.M., Lee, H.K., Nam, H.J., Lee, J.-Y., Cho, J.H., Moon, J.H., Lee, G.S., Kim, J.-H., Kim, P., Park, C.H., Hwang, J.Y. (2022) Structure-activity relationship analysis of novel GSPT1 degraders based on benzotriazinone scaffold and its antitumor effect on xenograft mouse model., Bioorganic Chemistry, Vol 127, 105923, https://doi.org/10.1016/j.bioorg.2022.105923
2021
20. Kim, Y.E., Kim, H.Y., Jung, D., Kang, D., Nam, D.-H., Nam, H.J.*, Cho, H.* (2021) Glioblastoma patient-derived cell-based phenotypic drug screening and identification of possible action mechanisms through proteomic analysis., STAR Protocols, Vol 2, Issue 4,100849. https://doi.org/10.1016/j.xpro.2021.100849 (*Co-corresponding author)
Graphical Abstract
Graphical Abstract
19. Nam, H.J., Baek, S.H. (2021) Epigenetic Regulation in Breast Cancer. In: Noh DY., Han W., Toi M. (eds) Translational Research in Breast Cancer. Advances in Experimental Medicine and Biology, Vol 1187. Springer, Singapore. https://doi.org/10.1007/978-981-32-9620-6_5
18. Nam, H.J. (2021) Autophagy Modulators in Cancer: Focus on Cancer Treatment, Life, 11(8), 839, https://doi.org/10.3390/life11080839
17. Nam, H.J., Kim, Y.E., Moon, B.-S., Kim, H.Y., Jung, D., Choi, S., Jang, J.W., Nam, D.-H., and Cho, H. (2021) Azathioprine antagonizes aberrantly elevated lipid metabolism and induces apoptosis in glioblastoma. iScience 24, 102238, https://doi.org/10.1016/j.isci.2021.102238
Illustrative Images Depicting Research Contents
Illustrative Images Depicting Research Contents
2020
16. Go, A., Jang, J.W., Lee, W., Ha, J.D., Kim, H.J.*, Nam, H.J.* (2020) Augmentation of the antitumor effects of PARP inhibitors in triple negative breast cancer via degradation by hydrophobic tagging modulation. European Journal of Medicinal Chemistry 204 (*Co-corresponding author)
2019
15. Choi, K.J., Na, Y.-J., Jung, W.H., Park, S.B., Kang S., Nam, H.J., Ahn, J.H., Kim, K.Y. (2019) Protective effect of a novel selective 11β-HSD1 inhibitor on eye ischemia reperfusion induced glaucoma. Biochemical Pharmacology 196
14. Nam, H.J. and Baek, S.H. (2019) Epigenetic regulation of the hypoxic response. Current Opinion in Physiology 7, 1-8 (Selected as a Cover Article)
Cover Image
Cover Image
2018
13. Kim, D.*, Nam, H.J.*, Lee, W., Yim, H.Y., Ahn, J.-Y., Park, S.W., Shin, H.-J.R., Yu, R., Won, K.-J., Bae, J.-S., Kim, K.I., Baek, S.H. (2018) PKCa-LSD1-NFkB Signaling Cascade Is Crucial for Epigenetic Control of the Inflammatory Response. Molecular Cell 69, 398-411 (*Co-first author) : 100회 이상 피인용
Graphical Abstract
Graphical Abstract
2017
12. Lim, C.-S.*, Nam, H.J.*, Lee, J., Kim, D., Choi, J., Kang, S.J., Kim, S., Kim, H., Kwak, C., Shim, K.-W., Kim, S., Ko, H.-G., Lee, R.U., Jang, E.-H., Yoo, J., Shim, J., Islam, A., Lee, J.-H., Baek, S.H., Kaang, B.-K. (2017) PKCa-mediated phosphorylation of LSD1 is required for presynaptic plasticity and hippocampal learning and memory. Scientific Reports 7, doi:10.1038/s41598-017-05239-7, (*Co-first author)
11. Kim, K., Lee, J.M., Yu, Y.S., Kim, H., Nam, H.J., Moon, H.-G., Noh, D.-Y., Kim, K.I., Fang, S., Baek, S.H. (2017) RORa2 requires LSD1 to enhance tumor progression in breast cancer. Scientific Reports 7, doi:10.1038/s41598-017-12344-0
10. Lim, C.-S., Kim, H., Yu, N.-K., Kang, S.J., Kim, T., Ko, H.-G., Lee, J., Yang, J.-e., Ryu, H.-H., Park, T., Gim, J., Nam, H.J., Baek, S.H., Wegener, S., Schmitz, D., Boeckers, T.M., Lee, M.G., Kim, E., Lee, J.-H., Lee, Y.-S., Kaang, B.-K. (2017) Enhancing inhibitory synaptic function reverses spatial memory deficits in Shank2 mutant mice. Neuropharmacology doi: 10.1016/j.neuropharm.2016
2016
9. Kim, Y.*, Nam, H.J.*, Lee, J., Park, D.Y., Kim, C., Yu, Y.S., Kim, D., Park, S.W., Bhin, J., Hwang, D., Lee, H., Koh, G.Y. and Baek, S.H. (2016) Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis. Nature Communications 7, doi:10.1038/ncomms10347 (*Co-first author): 250회 이상 피인용
SET7/9-dependent methylation and LSD1-dependent demethylation of HIF-1a regulate protein stability primarily in the nucleus in a hydroxylation- and VHL-independent manner during normoxia and long-term hypoxia.
2014
8. Nam, H.J., Boo, K., Kim, D., Han, D.H., Choe, H.K., Kim, C.R., Sun, W., Kim, H., Kim, K., Lee, H., Metzger, E., Schuele, R., Yoo, S., Takahashi, J. S., Cho, S., Son, G.H., and Baek, S. H. (2014) Phosphorylation of LSD1 by PKCa is crucial for circadian rhythmicity and phase resetting. Molecular Cell 53, 791-805 (First author, Selected as a Cover Article, Faculty of 1000 Recommended): 110회 이상 피인용
Cover Image
Cover Image
언론 보도: 잠 못드는 수면장애... 치료길 열리나, Molecular Cell 誌 표지논문 선정, 신개념 수면장애 치료제 개발 가능성 제시
https://m.science.ytn.co.kr/program/view.php?mcd=0082&key=201402281536433893
https://www.youtube.com/watch?v=9erW2bWmlZY
http://www.bosa.co.kr/news/articleView.html?idxno=492504
https://www.edaily.co.kr/News/Read?newsId=01108646605995112&mediaCodeNo=257
https://news.mt.co.kr/mtview.php?no=2014022716353396650
https://www.asiae.co.kr/article/2014022618553076955&mobile=Y
https://m.dongascience.com/news.php?idx=3886
https://www.mk.co.kr/news/it/5960025
7. Choi, J.H., Yu, N.K., Baek, G.C., Bakes, J., Seo, D., Nam, H.J., Baek, S.H., Lim, C.-S., Lee, YS., and Kaang, B.-K. (2014) Optimization of AAV expression cassettes to improve packaging capacity and transgene expression in neurons. Molecular Brain 7:17.
2013
6. Park, M.S., Kim, W.K., Song, M., Park, M.H., Kim, H.K., Nam, H.J., Baek, S.H., and Kim, H.G. (2013) Protein kinase C-delta-mediated recycling of active KIT in colon cancer. Clinical Cancer Research 19, 4961-4971.
2011
5. Kim, B., Nam, H.J., Pyo, K E., Jang, M.J., Kim, I.S., Kim, D., Boo, K., Lee, S.H., Yoon, J.B., Baek, S.H., and Kim, J.H. (2011) Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex. Biochem. Biophys. Res. Comm. 415, 720-726. IF: 2.297
4. Lee, J.S., Kim, Y., Bhin, J.H., Shin, H-J.R., Nam, H.J., Lee, S.H., Yoon, J.-B., Binda, O., Gozani, O., Hwang, D.H., and Baek, S.H. (2011) Hypoxia induced methylation of a pontin chromatin remodelling factor. Proc. Natl. Acad. Sci. USA 108, 13510-13515.
2010
3. Choi, H.J., Lee, J.M., Kim, H.K., Nam, H.J., Shin, H-J.R., Kim, D.H., Ko, E.Y., Noh, D.Y., Kim, K.I., Kim, J.H., and Baek, S.H. (2010) Bcl3-dependent stabilization of CtBP1 is crucial for the inhibition of apoptosis and tumor progression in breast cancer. Biochem. Biophys. Res. Comm. 400, 396-402.
2. Oh, S.M.*, Nam, H.J. *, Park, J.M. *, Baek, S.H., and Park, S.B. (2010) Development of a benzopyran-containing androgen receptor antagonist to treat antiandrogen-resistant prostate cancer. Chem. Med. Chem. 5, 529-533. (*Co-first author)
2007
Kim, J.H., Lee, J.M., Nam, H.J., Choi, H.J., Yang, J.W., Lee, J.S., Kim, M.H., Kim, S.-I., Chung, C.H., Kim, K.I., and Baek, S.H. (2007) SUMOylation of a pontin chromatin remodeling complex reveals a new signal integration code in prostate cancer. Proc. Natl. Acad. Sci. USA. 104, 20793-20798.
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