ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization. ☆

Nakamura H, Rho E, Lee CT, Itoh K, Deng D, Watanabe S, Razavi S, Matsubayashi HT, Zhu C, Jung E, Rangamani P, Watanabe S, Inoue T. 

Cell Rep. 2023 Sep 2;:113089. doi: 10.1016/j.celrep.2023.113089. [Epub ahead of print] PubMed PMID: 37734382.

Rapid manipulation of mitochondrial morphology in a living cell with iCMM. 

Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H. 

Cell Rep Methods. 2021 Aug 23;1(4):100052. doi: 10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23. PubMed PMID: 35475143; PubMed Central PMCID: PMC9017203.

Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules. 

Nakamura H. ☆

Methods Mol Biol. 2021;2312:253-276. doi: 10.1007/978-1-0716-1441-9_15. PubMed PMID: 34228295.

The Loss of Nuclear PTEN Increases Tumorigenesis in a Preclinical Mouse Model for Hepatocellular Carcinoma. 

Kato T, Yamada T, Nakamura H, Igarashi A, Anders RA, Sesaki H, Iijima M. 

iScience. 2020 Oct 23;23(10):101548. doi: 10.1016/j.isci.2020.101548. eCollection 2020 Oct 23. PubMed PMID: 33083717; PubMed Central PMCID: PMC7516300.

Rational design and implementation of a chemically inducible heterotrimerization system. ☆

Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T. 

Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x. Epub 2020 Aug 3. PubMed PMID: 32747768; PubMed Central PMCID: PMC9936427.

Harnessing biomolecular condensates in living cells. ☆

Nakamura H, DeRose R, Inoue T. 

J Biochem. 2019 Jul 1;166(1):13-27. doi: 10.1093/jb/mvz028. Review. PubMed PMID: 31020316; PubMed Central PMCID: PMC6821259.

Stimulation-induced changes in diffusion and structure of calmodulin and calmodulin-dependent protein kinase II proteins in neurons. 

Heidarinejad M, Nakamura H, Inoue T. 

Neurosci Res. 2018 Nov;136:13-32. doi: 10.1016/j.neures.2018.01.003. Epub 2018 Feb 1. Review. PubMed PMID: 29395358.

Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. ☆

Nakamura H, Lee AA, Afshar AS, Watanabe S, Rho E, Razavi S, Suarez A, Lin YC, Tanigawa M, Huang B, DeRose R, Bobb D, Hong W, Gabelli SB, Goutsias J, Inoue T. 

Nat Mater. 2018 Jan;17(1):79-89. doi: 10.1038/nmat5006. Epub 2017 Nov 6. PubMed PMID: 29115293; PubMed Central PMCID: PMC5916848.

Optical detection of neuron connectivity by random access two-photon microscopy. 

Shafeghat N, Heidarinejad M, Murata N, Nakamura H, Inoue T. 

J Neurosci Methods. 2016 Apr 1;263:48-56. doi: 10.1016/j.jneumeth.2016.01.023. Epub 2016 Feb 3. PubMed PMID: 26851307.

Toward total synthesis of cell function: Reconstituting cell dynamics with synthetic biology. ☆

Kim AK, DeRose R, Ueno T, Lin B, Komatsu T, Nakamura H, Inoue T. 

Sci Signal. 2016 Feb 9;9(414):re1. doi: 10.1126/scisignal.aac4779. Review. PubMed PMID: 26861045.

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo. 

Inoue T, Narita K, Nonami Y, Nakamura H, Takeda S. 

J Vis Exp. 2015 Jul 13;(101):e52991. doi: 10.3791/52991. PubMed PMID: 26273731; PubMed Central PMCID: PMC4544892.

TRPV4 regulates the integrity of the blood-cerebrospinal fluid barrier and modulates transepithelial protein transport. 

Narita K, Sasamoto S, Koizumi S, Okazaki S, Nakamura H, Inoue T, Takeda S. 

FASEB J. 2015 Jun;29(6):2247-59. doi: 10.1096/fj.14-261396. Epub 2015 Feb 13. PubMed PMID: 25681460.

Cationic amino acid based lipids as effective nonviral gene delivery vectors for primary cultured neurons. 

Aoshima Y, Hokama R, Sou K, Sarker SR, Iida K, Nakamura H, Inoue T, Takeoka S. 

ACS Chem Neurosci. 2013 Dec 18;4(12):1514-9. doi: 10.1021/cn400036j. Epub 2013 Oct 11. PubMed PMID: 24087930; PubMed Central PMCID: PMC3867963.

Developmental changes in ciliary motility on choroid plexus epithelial cells during the perinatal period. 

Nonami Y, Narita K, Nakamura H, Inoue T, Takeda S. 

Cytoskeleton (Hoboken). 2013 Dec;70(12):797-803. doi: 10.1002/cm.21132. Epub 2013 Sep 20. PubMed PMID: 23959957.

Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention. 

Takei Y, Murata A, Yamagishi K, Arai S, Nakamura H, Inoue T, Takeoka S. 

Chem Commun (Camb). 2013 Aug 25;49(66):7313-5. doi: 10.1039/c3cc42489h. PubMed PMID: 23851490.

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier. ☆

Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, Inoue T. 

Nat Chem Biol. 2013 Jul;9(7):437-43. doi: 10.1038/nchembio.1252. Epub 2013 May 12. PubMed PMID: 23666116; PubMed Central PMCID: PMC3870470.

Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals. 

Narita K, Kozuka-Hata H, Nonami Y, Ao-Kondo H, Suzuki T, Nakamura H, Yamakawa K, Oyama M, Inoue T, Takeda S. 

Biol Open. 2012 Aug 15;1(8):815-25. doi: 10.1242/bio.20121081. Epub 2012 Jun 29. PubMed PMID: 23213475; PubMed Central PMCID: PMC3507226.

Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact Hela cells. 

Nakamura H, Bannai H, Inoue T, Michikawa T, Sano M, Mikoshiba K. 

J Biol Chem. 2012 Jul 13;287(29):24563-72. doi: 10.1074/jbc.M111.311399. Epub 2012 May 25. PubMed PMID: 22637479; PubMed Central PMCID: PMC3397881.

Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells. 

Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K. 

J Biol Chem. 2011 Jun 10;286(23):20591-9. doi: 10.1074/jbc.M110.204685. Epub 2011 Apr 22. PubMed PMID: 21515674; PubMed Central PMCID: PMC3121519.

Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 is regulated by actin filaments and 4.1N in neuronal dendrites. 

Fukatsu K, Bannai H, Zhang S, Nakamura H, Inoue T, Mikoshiba K. 

J Biol Chem. 2004 Nov 19;279(47):48976-82. doi: 10.1074/jbc.M408364200. Epub 2004 Sep 10. PubMed PMID: 15364918.

日本語総説

生きた細胞内に人工のハイドロゲル凝縮体をつくる合成生物学技術iPOLYMER

中村 秀樹

生物物理 2021 年 61 巻 3 号 p. 166-170