Extracellular matrix (ECM) is a complex of proteins and and carbohydrates deposited in outside of the cell. ECM not only plays an important role in creating adhesive environment for tissue and cells but also it binds to the cell and regulates tissue organization such as cell adhesion, migration, differentiation, and contraction. Many ECM components, such as fibronectin and laminin, interacts with cell using their cell binding motif. The well-known example is RGD motif present in fibronectin. We are interested in studying the function of cell binding motifs in ECM molecules. We found many ECM glycoproteins include RGD motifs in their immunoglobulin-like domains. Using CRISPR techniques, we are creating substitution mutations in such motifs and identify mutations causing defective function of integrins, a cell surface receptor for ECM molecules.