The physical (wet) lab is located within the College of Public Health laboratory suite in the Iowa Biomedical Innovation Faciligy (IBIF), located with the University of Iowa mecial campus. 

The newly renovated, pressure-controlled lab consists of five connected rooms with a combined footprint of 900 square feet. 

The entry chamber is used for preparing and receiving shipments (samples, collection kits, supplies), including a workbench with built-in storage, a -80C freezer, and label printer. 

The attached antechamber is used for sample processing and DNA extraction, housing a biosafety cabinet for BSL2 samples, a chemical safety cabinet for volatile reagents, centrifuges, homogenizers, and a refrigerator for reagent storage. 

The primary laboratory space, attached to the entry chamber, is used for sequencing library preparation, qPCR, and protein assays. The space has several benchtop workstations, refrigerator, freezers, and the necessary equipment for DNA and protein purification, quantification, and visualization. 

The exit chamber serves primarily as a storage space, with an antechamber used for bacterial culturing, including an anaerobic culturing chamber, temperature- and humidity-controlled incubators, and a -80C freezer for sample storage. 

The College of Public Health laboratory suite also includes a general utility room for shared equipment like autoclaves, a water purification system, laundry and dish washing equipment, and a dedicated freezer farm, and shared access to the Molecular Biology Lab withint the Environmental Health Sciences Research Center lab facilities.

• Hamilton Chemical Fume Hood

• Labconco Biological Safety Cabinet

• Bactron 300 Anaerobic Chamber

• Eppendorf Centrifuge 5425R

• Eppendorf Centrifuge 5810R

• Eppendorf Pipettes – single channel, multi-channel, and repeater

• Mettler Analytical Balance XSR105

• Mettler Precision Balance ME6002T

• Thermo Scientific Vios iDx 165 L CO2 Incubator

• Invitrogen Qubit 4.0

• QuantStudio 3 Real-Time PCR System

• Synergy HI Mono Plate Reader

• Bio-Rad Tempo 96 Thermal Cycler

• Bio-Rad Tempo 48/48 Thermal Cycler

• Bio-Rad Tempo 384 Thermal Cycler

• Bio-Rad PX1 PCR Plate Sealer

• Bio-Rad Gel Electrophoresis Equipment

• Panasonic and Thermo Ultra Cold Freezers

• Fisher Scientific Combo Fridge/Freezer, and VWR Under Bench Fridge and Freezer

• inGenius 3 Gel Doc System with Blue LED Transilluminator

• Fisher Scientific Bead Mill 24

• VWR PCR Workstation

• Fisher Scientific Mini Centrifuge and Vortexer

• Fisher Scientific Microplate Shaker

• Liquid Nitrogen Tank (for flash freezing and frozen homogenization)