Lab Facilities
The primary functions of the Microbial Exposomics Laboratory facilities are to 1) process and prep microbiome samples for sequencing, and 2 ) perform in vitro experiments on bacterial cultures.
Space
The physical (wet) lab is located within the University of Iowa College of Public Health laboratory suite in the Oakdale Multi-Tenant Facility.
The lab consists of two rooms with a combined footprint of approximately 1,100 square feet. One room is for sample processing, DNA extraction, and bacterial culturing, and one is for DNA replication and library prep for sequencing.
The processing room includes a biosafety cabinet for BSL2 samples, a chemical safety cabinet for volatile reagents, an anaerobic culturing chamber, incubators, centrifuges, and homogenizers, as well as several bench-top workstations, refrigerators, and freezers for sample and reagent storage.
The library prep room has several bench-top workstations, refrigerators, freezers, and the necessary equipment for DNA purification, replication, quantification, and visualization.
The College of Public Health laboratory suite also includes multiple shared equipment rooms for large equipment like autoclaves, as well as additional office space for Dr. Eggers and her laboratory staff.
Equipment
Hamilton Chemical Fume Hood
Fisher Scientific Biological Safety Cabinet
Bactron 300 Anaerobic Chamber
Eppendorf Centrifuge 5425R
Eppendorf Centrifuge 5810R
Eppendorf Pipettes – single channel, multi-channel, and repeater
Mettler Analytical Balance XSR105
Mettler Precision Balance ME6002T
Invitrogen Qubit 4.0
Synergy HI Mono Plate Reader
Bio-Rad Tempo 96 Thermal Cycler
Bio-Rad Tempo 48/48 Thermal Cycler
Bio-Rad Tempo 384 Thermal Cycler
Bio-Rad PX1 PCR Plate Sealer
Bio-Rad Gel Electrophoresis Equipment
Panasonic and Thermo Ultra Cold Freezers
Fisher Scientific Combo Fridge/Freezer, and VWR Under Bench Fridge and Freezer
inGenius 3 Gel Doc System with Blue LED Transilluminator
Fisher Scientific Bead Mill 24
VWR PCR Workstation
Fisher Scientific Mini Centrifuge and Vortexer
Fisher Scientific Microplate Shaker
Liquid Nitrogen Tank (for flash freezing and frozen homogenization)
Have questions? Contact the lab manager, Joe Eggers, at joseph-eggers@uiowa.edu