Scaffold Optimization
Scaffold Optimization
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Promotion of lymph vessel growth via attachment of Vascular Endothelial Growth Factor (VEGF )
Promotion of lymph vessel growth via attachment of Vascular Endothelial Growth Factor (VEGF )
The loading of VEGF was quantified using ELISA by the amount of VEGF left in the media after incubation with and without lymph nodes.
The loading of VEGF was quantified using ELISA by the amount of VEGF left in the media after incubation with and without lymph nodes.
The VEGF left in the media was significantly less in media incubated with lymph nodes than in media only, indicating that more than 50% of VEGF was able to attach to decellularized lymph nodes. Heparin cross-linking did not increase VEGF loading in lymph nodes.
The VEGF left in the media was significantly less in media incubated with lymph nodes than in media only, indicating that more than 50% of VEGF was able to attach to decellularized lymph nodes. Heparin cross-linking did not increase VEGF loading in lymph nodes.
The release of VEGF was quantified by placing the lymph node into fresh media and testing the VEGF concentration in the media over time.
The release of VEGF was quantified by placing the lymph node into fresh media and testing the VEGF concentration in the media over time.
The lymph nodes released most of the VEGF during the first 24 hours. After 48 hours, 80% of the VEGF was released. No significant difference was observed between lymph nodes with and without heparin cross-linking for either VEGF loading or release.
The lymph nodes released most of the VEGF during the first 24 hours. After 48 hours, 80% of the VEGF was released. No significant difference was observed between lymph nodes with and without heparin cross-linking for either VEGF loading or release.
VEGF is able to attach and release from the lymph node scaffold.
VEGF is able to attach and release from the lymph node scaffold.
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