Publications

Hwang HY, Mariam N, Kweon J, Yi H, Jang G, Roh J, Lim K, Park JH, Kim Y, & Kim D. (2026) Structure-guided engineering of AI-derived adenine base editors for nuclear and mitochondrial DNA editing. Nucleic Acids Research, 54(14). https://doi.org/10.1093/nar/gkag737 

Kim MG, Go MJ, Kang SH, Jeong SH & Lim K#. (2025) Revolutionizing CRISPR technology with artificial intelligence. Experimental & Molecular Medicine, 57, 1419-1431. https://doi.org/10.1038/s12276-025-01462-9 [#Corresponding author]

Kweon J, Park S, Jeon MY, Lim K, Jang G, Jang AH, Lee M, Seok C, Lee C, Park S, Ahn J, Jang J, Kim N, Sung YH, Kim D, & Kim Y. (2025) High-efficiency base editing for nuclear and mitochondrial DNA with an optimized DYW-like deaminase. Molecular Therapy, 33(11), 5611-5623. https://doi.org/10.1016/j.ymthe.2025.08.007

Hwang HY, Lee M, Yi H, Seok C, Lim K, Na YR, Kang JS, Park JH & Kim D. (2025) Engineered Sdd7 cytosine base editors with enhanced specificity. Nature Communications, 16, 5881. https://doi.org/10.1038/s41467-025-60789-z 

Lee S, Kim K, Jeong HJ, Choi S, Cheng H, Kim D, Heo S, Mun J, Kim M, Lee E, Choi YJ, Lee SG, Lee EA, Jang Y, Lim K, Kim HS, Jeong E, Myung SJ, Jung DB, Yu CS, Song IH, Corces MR, Kang JH, Myung K, Kwon T, Park TE, Joo J & Cho SW. (2025) Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells. Cancer Research, 85(15), 2890-2904. https://doi.org/10.1158/0008-5472.CAN-24-2938 

Yousefian-Jazi A, Kim S, Chu J, Choi SH, Nguyen PTT, Park U, Kim MG, Hwang H, Lee K, Kim Y, Hyeon SJ, Rhim H, Ryu HL, Lim G, Stein TD, Lim K, Ryu H, & Lee J. (2025) Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice. Molecular Neurodegeneration, 20(1), 16. https://doi.org/10.1186/s13024-024-00792-y

Cho SI*, Lim K*, Hong S*, Lee J, Kim A, Lim CJ, Ryou S, Lee JM, Mok YG, Chung E, Kim S, Han S, Cho SM, Kim J, Kim EK, Nam KH, Oh Y, Choi M, An TH, Oh KJ, Lee S#, Lee H#, & Kim JS#. (2024) Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNA. Cell, 187(1), 95-109 e126. https://doi.org/10.1016/j.cell.2023.11.035  [*Co-first author]

Lim K#. (2024) Mitochondrial genome editing: strategies, challenges, and applications. BMB Reports, 57(1), 19-29. https://doi.org/10.5483/BMBRep.2023-0224  [#Corresponding author]

Lee J, Lim K*, Kim A, Mok YG, Chung E, Cho SI, Lee JM, & Kim JS. (2023) Prime editing with genuine Cas9 nickases minimizes unwanted indels. Nature Communications, 14(1), 1786. https://doi.org/10.1038/s41467-023-37507-8  [*Co-first author]

Lim K*, Cho SI*, & Kim JS. (2022) Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases. Nature Communications, 13(1), 366. https://doi.org/10.1038/s41467-022-27962-0  [*Co-first author]

Cho SI, Lee S, Mok YG, Lim K, Lee J, Lee JM, Chung E, & Kim JS. (2022) Targeted A-to-G base editing in human mitochondrial DNA with programmable deaminases. Cell, 185(10), 1764-1776 e1712. https://doi.org/10.1016/j.cell.2022.03.039

Whisenant D*, Lim K*, Revechon G, Yao H, Bergo MO, Machtel P, Kim JS, & Eriksson M. (2022) Transient expression of an adenine base editor corrects the Hutchinson-Gilford progeria syndrome mutation and improves the skin phenotype in mice. Nature Communications, 13(1), 3068. https://doi.org/10.1038/s41467-022-30800-y  [*Co-first author]

Mok YG, Lee JM, Chung E, Lee J, Lim K, Cho SI, & Kim JS. (2022) Base editing in human cells with monomeric DddA-TALE fusion deaminases. Nature Communications, 13(1), 4038. https://doi.org/10.1038/s41467-022-31745-y

Gim GM, Kwon DH, Eom KH, Moon J, Park JH, Lee WW, Jung DJ, Kim DH, Yi JK, Ha JJ, Lim K, Kim JS, & Jang G. (2022) Production of MSTN-mutated cattle without exogenous gene integration using CRISPR-Cas9. Biotechnology Journal, 17(7), e2100198. https://doi.org/10.1002/biot.202100198

Cho E, Cheon S, Ding M, Lim K, Park SW, Park C, & Lee TH. (2022) Identification of Novel Genes for Cell Fusion during Osteoclast Formation. International Journal of Molecular Sciences, 23(12). https://doi.org/10.3390/ijms23126421

Hwang GH, Jeong YK, Habib O, Hong SA, Lim K, Kim JS, & Bae S. (2021) PE-Designer and PE-Analyzer: web-based design and analysis tools for CRISPR prime editing. Nucleic Acids Research, 49(W1), W499-W504. https://doi.org/10.1093/nar/gkab319

Kim D*, Lim K*, Kim DE, & Kim JS. (2020) Genome-wide specificity of dCpf1 cytidine base editors. Nature Communications, 11(1), 4072. https://doi.org/10.1038/s41467-020-17889-9  [*Co-first author]

Hwang GH, Yu J, Yang S, Son WJ, Lim K, Kim HS, Kim JS, & Bae S. (2020) CRISPR-sub: Analysis of DNA substitution mutations caused by CRISPR-Cas9 in human cells. Computational and Structural Biotechnology Journal, 18, 1686-1694. https://doi.org/10.1016/j.csbj.2020.06.026

Ryu SM*, Koo T*, Kim K*, Lim K*, Baek G, Kim ST, Kim HS, Kim DE, Lee H, Chung E, & Kim JS. (2018) Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nature Biotechnology, 36(6), 536-539. https://doi.org/10.1038/nbt.4148  [*Co-first author]

Hwang GH, Park J, Lim K, Kim S, Yu J, Yu E, Kim ST, Eils R, Kim JS, & Bae S. (2018) Web-based design and analysis tools for CRISPR base editing. BMC Bioinformatics, 19(1), 542. https://doi.org/10.1186/s12859-018-2585-4

Kim D*, Lim K*, Kim ST, Yoon SH, Kim K, Ryu SM, & Kim JS. (2017) Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nature Biotechnology, 35(5), 475-480. https://doi.org/10.1038/nbt.3852  [*Co-first author]

Kim K, Ryu SM, Kim ST, Baek G, Kim D, Lim K, Chung E, Kim S, & Kim JS. (2017) Highly efficient RNA-guided base editing in mouse embryos. Nature Biotechnology, 35(5), 435-437. https://doi.org/10.1038/nbt.3816

Park J, Lim K, Kim JS, & Bae S. (2017) Cas-analyzer: an online tool for assessing genome editing results using NGS data. Bioinformatics, 33(2), 286-288. https://doi.org/10.1093/bioinformatics/btw561