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Using the 2005 Leaf Train coordinates as a guideline, we're stopping every 50 km as we travel north and west through the boreal forest. We're targeting young, moderately dense forest, with trees no taller than 6m. We're avoiding bogs and lowland terrain.
With our baseline criteria met, we start our work!
Take a GPS coordinate
Write down notes about the location
Collect soil and leaf litter from around the trees
Next, we flag our target species, measure their size and height, count needle cohorts (for conifers: years of growth that are still hanging on to their needles), and take a core. Finally, we clip a branch from the southern (sunny) side in the top 1/3rd of the canopy.
Back at the RV, leaves go into bags with damp paper towels to keep the leaves hydrated. We keep them cool with ice so they stay fresh until we weigh them.
At the end of a day, we weigh our leaf and needle samples from each site. We scan them to measure their size and shape.
Once a sample's been scanned and weighed, it goes off to a 60°C dehydrating oven. Drying the samples stops respiration and preserves the carbon to nitrogen ratio so we can correctly analyze our samples upon our return.