Projects
scRNA-seq has been a trend in biotechnology, and very useful for dissecting complex cellular identities and genetic landscape. We recently applied 10X Genomics system to identify a specific cell subtype in heterogenous tissue of oral& maxillofacial region.
16S rRNA amplicon sequencing targets a specific region of the 16S rRNA gene to profile microbial communities. Using specific primers, this region is amplified and sequenced using a next-generation platform. Bioinformatics tools then process the data to identify and classify the microbial species present.
We set the useful system to visualize cell signaling dynamics in diverse biological contexts using fluorescent protein-based biosensors. Above movie shows actin dynamics in endothelial cells according to localized stimulation (Kim JM et al. PNAS, 2016)
The SNU Dental Multiomics Center (DMC) comprises a pioneering group of seven dental specialists who lead the application of state-of-the-art next-generation sequencing (NGS) technology in the field of dental science. Within our consortium, each specialist focuses on specific multiomics fields, including genomics, epigenomics, transcriptomics, and proteomics, which enhances the professionalism of our center. Both our members and external clients can receive guidance throughout the entire process of diverse sequencing technologies such as RNA-Seq, ATAC-seq, WES, and more. Notably, DMC is fully equipped with the entire pipeline for oral microbiome studies, encompassing 16S rRNA amplicon and shotgun metagenomic sequencing. Various samples and specimens collected from the oromaxillofacial region are required to be stored at the SNU Dental Hospital Oral Tissue Bank, and sequencing information is subsequently deposited into the bank as secondary resources. This deposited database can be integrated with patient data to reveal clinical significance. The overarching goal of DMC is to provide all the necessary resources to achieve successful multiomics studies, spanning from microbes to hosts and from basic to translational research.
*Contact information: jinmankim@snu.ac.kr (research coordinator); https://dri.snu.ac.kr/ (Dental Multiomics Center)