Experimental Skills

1. Know-how

• Establishing and cultivating photo/mixo/heterotrophic protists

• Feeding & competition experiments (phototrophy, mixotrophy, heterotrophy)

• Growth rates as a function of prey or nutrient concentrations

• Temperature, salinity, and light response

• Warming and eutrophication effects experiments (environmental samples)

• Identification and enumerating protists (environmental samples)

• Enumerating bacteria and nanoflagellates (environmental samples)

• Mycelium fermentation (for food application)

2. Techniques

• Nutrients (QuAAtro, Seal Analytical)

• Chl-a (Turner 10AU)

• LM, SEM, TEM

• DNA (16S, 18S)

• qPCR (Rotor-Gene Q 2plex, Qiagen)

• Quantitative Protargol Staining  (ciliate classification)