PUBLICATION

主要業績

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精子走化性・運動調節に関する主な研究成果

Shiba K, Inaba K.  (2023) The role of soluble adenylyl cyclase in the regulation of flagellar motility in ascidian sperm. Biomolecules 13(11), 1594. 

Shiba K, Baba SA, Fujiwara E, Inaba K.  (2023) Calaxin is required for asymmetric bend initiation and propagation in sperm flagella. Front Cell Dev Biol. 11:1136404. 

Shiba K, Inaba K.  (2022) The roles of two CNG channels in the regulation of ascidian sperm chemotaxis. Int J Mol Sci. 23(3): 1648.

Yoshida K, Shiba K, Sakamoto A, Ikenaga J, Matsunaga S, Inaba K, Yoshida M. (2018) Ca²⁺ efflux via plasma membrane Ca²⁺-ATPase mediates chemotaxis in ascidian sperm. Sci Rep.8(1):16622. 

Shiba K, Inaba K. (2014) Distinct roles of soluble and transmembrane adenylyl cyclases in the regulation of flagellar motility in ciona sperm. Int J Mol Sci., 15(8): 13192-208.

Shiba K, Shibata D, Inaba K. (2014) Autonomous changes in the swimming direction of sperm in the gastropod Strombus luhuanus. J Exp Biol., 217(Pt 6): 986-96. 

Mizuno K, Shiba K, Okai M, Takahashi Y, Shitaka Y, Oiwa K, Tanokura M, Inaba K. (2012) Calaxin drives sperm chemotaxis by Ca²⁺-mediated direct modulation of a dynein motor. Proc Natl Acad Sci U S A., 109(50): 20497-502. 

Shiba K, Baba SA, Inoue T, Yoshida M.  (2008) Ca²⁺ bursts occur around a local minimal concentration of attractant and trigger sperm chemotactic response. Proc Natl Acad Sci USA 105, 19311-19316.

Yoshida M, Shiba K, Yoshida K, Tsuchikawa H, Ootou O, Oishi T, Murata M. Ascidian sperm activating and attracting factor: importance of sulfate groups for the activities and implication of its putative receptor. FEBS letters 582, 3429-3433

Shiba K, Marian T, Krasznai Z, Baba SA, Morisawa M, Yoshida M.  (2006) Na⁺/Ca²⁺ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa. Cell Motility and the Cytoskeleton 63, 623-632.

Shiba K, Tagata T, Ohmuro J, Mogami Y, Matsumoto M, Hoshi M, Baba SA.  (2006) Peptide induced hyperactivation-like vigorous flagellar movement in starfish sperm. Zygote 14, 23-32.

Shiba K, Ohmuro J, Mogami Y, Nishigaki T, Wood CD, Darszon A, Tatsu Y, Yumoto N, Baba SA. (2005) Sperm-activating peptide induces asymmetric flagellar bending in sea urchin sperm. Zool Sci 22, 293-299.

Nishigaki T, Wood CD, Tatsu Y, Yumoto N, Furuta T, Elias D, Shiba K, Baba SA, Darszon A.  (2004) A Sea Urchin Egg Jelly Peptide Induces a cGMP-Mediated Decrease in Sperm Intracellular Ca²⁺ Prior to Its Increase. Dev Biol 272, 376-388.

鞭毛・繊毛の機能に関する主な研究成果

Shibata A, Yamamoto C, Tani Y, Yanase R, Shiba K, Sato Y, Yabuki A, Inaba K. The photophobic response in the apusomonad Podomonas kaiyoae is mediated by coordination of cilia and actin filaments. Commun Biol. 2025 Dec 18;8(1):1787. 

Sasaki K, Shiba K, Nakamura A, Kawano N, Satouh Y, Yamaguchi H, Morikawa M, Shibata D, Yanase R, Jokura K, Nomura M, Miyado M, Takada S, Ueno H, Nonaka S, Baba T, Ikawa M, Kikkawa M, Miyado K, Inaba K. Calaxin is required for cilia-driven determination of vertebrate laterality. Commun Biol. 2019 Jun 20;2:226. 

Jokura K, Sato Y, Shiba K, Inaba K. Two distinct compartments of a ctenophore comb plate provide structural and functional integrity for the motility of giant multicilia. Curr Biol. 2022 Oct 19:S0960-9822(22)01603-7.

Kutomi O, Yamamoto R, Hirose K, Mizuno K, Nakagiri Y, Imai H, Noga A, Obbineni JM, Zimmermann N, Nakajima M, Shibata D, Shibata M, Shiba K, Kita M, Kigoshi H, Tanaka Y, Yamasaki Y, Asahina Y, Song C, Nomura M, Nomura M, Nakajima A, Nakachi M, Yamada L, Nakazawa S, Sawada H, Murata K, Mitsuoka K, Ishikawa T, Wakabayashi KI, Kon T, Inaba K. A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light. Sci Adv. 2021 Feb 26;7(9):eabf3621. 

Jokura K, Shibata D, Yamaguchi K, Shiba K, Makino Y, Shigenobu S, Inaba K. CTENO64 Is Required for Coordinated Paddling of Ciliary Comb Plate in Ctenophores. Curr Biol. 2019 Oct 21; 29(20):3510-3516.e4. 

Nomura M, Atsuji K, Hirose K, Shiba K, Yanase R, Nakayama T, Ishida KI, Inaba K. Microtubule stabilizer reveals requirement of Ca²⁺ -dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae. Biol Open. 2019 Feb 11;8(2). 

Mizuno K, Shiba K, Yaguchi J, Shibata D, Yaguchi S, Prulière G, Chenevert J, Inaba K. Calaxin establishes basal body orientation and coordinates movement of monocilia in sea urchin embryos. Sci Rep. 2017 Sep 7;7(1):10751. 

Shiba K, Inaba K. Inverse relationship of Ca²⁺-dependent flagellar response between animal sperm and prasinophyte algae. J Plant Res. 2017 May;130(3):465-473. 

Konno A, Shiba K, Cai C, Inaba K. Branchial cilia and sperm flagella recruit distinct axonemal components. PLoS One. 2015 May 11;10(5):e0126005.

Jin Y, Yaguchi S, Shiba K, Yamada L, Yaguchi J, Shibata D, Sawada H, Inaba K. (2013) Glutathione transferase theta in apical ciliary tuft regulates mechanical reception and swimming behavior of Sea Urchin Embryos. Cytoskeleton, 70: 453–470.

イメージング・波形解析の共同研究成果

Sugiura K, Shiba K, Inaba K, Matsumoto M. (2022) Morphological differences in tardigrade spermatozoa induce variation in gamete motility. BMC Zoology, 7(1): 8. 

Kinoshita-Terauchi N, Bellgrove A, Shiba K, Inaba K. (2021) Flagellar motility during sperm chemotaxis and phototaxis in fucalean algae. European Journal of Phycology 56:1, 85-93. 

Kinoshita-Terauchi N, Shiba K, Umezawa T, Matsuda F, Motomura T, Inaba K. (2019) A brown algal sex pheromone reverses the sign of phototaxis by cAMP/Ca²⁺-dependent signaling in the male gametes of Mutimo cylindricus (Cutleriaceae). J Photochem Photobiol B. 192:113-123. 

Momose T, De Cian A, Shiba K, Inaba K, Giovannangeli C, Concordet JP. (2018) High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion. Sci Rep. 8(1):11734. 

Kamiya R, Shiba K, Inaba K, Kato-Minoura T. (2018) Release of sticky glycoproteins from Chlamydomonas flagella during microsphere translocation on the surface membrane. Zoolog Sci. 35(4):299-305. 

Konno A, Ikegami K, Konishi Y, Yang HJ, Abe M, Yamazaki M, Sakimura K, Yao I, Shiba K, Inaba K, Setou M. (2016) Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice. J Cell Sci. 129(14):2757-66. 

Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, Fujihara Y, Isotani A, Inaba K, Ikawa M. (2015) Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 350(6259):442-5. 

Hirohashi N, Alvarez L, Shiba K, Fujiwara E, Iwata Y, Mohri T, Inaba K, Chiba K, Ochi H, Supuran CT, Kotzur N, Kakiuchi Y, Kaupp UB, Baba SA. (2013) Sperm from Sneaker Male Squids Exhibit Chemotactic Swarming to CO2. Curr Biol., 23(9): 775-81. 

Kikuchi A, Shiba K, Ozawa T, Nakano K, Inaba K, Numata O. (2012) Black Tea High-Molecular-Weight Polyphenol Increases the Motility of Sea Urchin Sperm by Activating Mitochondrial Respiration. Biosci Biotechnol Biochem., 76(12): 2321-4. 

Saito T, Shiba K, Inaba K, Yamada L, Sawada H. (2012) Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis. Proc Natl Acad Sci U S A., 109(11): 4158-62.

Kambara Y, Shiba K, Yoshida M, Sato C, Kitajima K, Shingyoji C. (2011) Mechanism regulating Ca²⁺-dependent mechanosensory behaviour in sea urchin spermatozoa. Cell Structure and Function 36, 69-82 .

Yaguchi S, Yaguchi J, Wei Z, Shiba K, Angerer LM, Inaba K.  (2010) ankAT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo. Developmental Biology 348, 67-75.

著書・総説

柴 小菊．精子運動の調節機構．稲葉一男（監修）, 『最新 精子学』，筑波大学出版会，2025年12月，pp.64-68． 

柴 小菊．細胞の行動．中垣　俊之・石川　拓司 (編), 『細胞の行動力学』，東京大学出版会，2025年12月，pp.1–45．

Shiba K. Regulatory mechanisms for sperm chemotaxis and flagellar motility. Genesis 2023 Sep 12:e23549. 

Inaba K, Shiba K. Microscopic analysis of sperm movement: links to mechanisms and protein components. Microscopy (Oxf). 2018 Jun 1;67(3):144-155. 

Inaba K, Kutomi O, Shiba K, Cosson J. Sperm guidance: comparison with motility regulation in bikont species. In: Flagellar Mechanics and Sperm Guidance (Cosson J, Ed), Bentham Science Publishers, 2015 Nov; pp.349-389. 

Inaba K, Mizuno K, Shiba K. (2014) Structure, Function, and Phylogenetic Consideration of Calaxin. Sexual Reproduction in Animals and Plants, pp 49-57.

稲葉 一男,  柴 小菊,  吉田 学（2014）精子運動の活性化と走化性. 

「動植物の受精学－共通機構と多様性」（澤田 均 編）、 化学同人、pp.286-300

水野 克俊,  柴 小菊,  稲葉 一男（2013）精子が卵を目指すしくみ―運動の方向転換を司るタンパク質「カラクシン」. 

遺伝, Vol. 67(5), 610-617. NTS

Shiba K, Mizuno K and Inaba K. Molecular comparison of the axonemal components between sperm flagella and Chlamydomonas flagella. Spermatology: new horizons in the 21 century (ed. M. Moriwsawa). Adthree Publishing Co. Ltd (2012), pp.30-40.

柴 小菊, 吉田 学 (2005)卵由来物質によるホヤ精子の運動制御機構. 号外海洋, 41, 98-104 海洋出版