DNA Practical Condensed Course
DNA Practical Condensed Course
DNA Practical Condensed Course
This condensed professional DNA practical course offers a concise, comprehensive introduction to essential molecular biology techniques. Participants will gain hands-on experience extracting and purifying DNA from Gram-negative, Gram-positive bacteria and avian blood cells. The curriculum emphasizes critical quality-control methods, including NanoDrop spectrophotometry and agarose gel electrophoresis, to ensure the accurate quantification and visualization of DNA samples. The curriculum emphasizes Polymerase Chain Reaction (PCR), which targets the 16S rRNA gene for microbial classification. This is a vital tool in biotechnology and genetics. Gel Documentation systems support precise data analysis and reinforcing reproducibility in research. By integrating theory with practice, the course equips learners to produce reliable, high-quality results applicable to biotechnology, microbial genetics, and molecular biology.
This intensive five-day program provides a hands-on introduction to essential molecular biology techniques. Participants will learn how to extract, purify, quantify, and analyze DNA from various biological sources, with a strong emphasis on reproducibility and data interpretation.
Target Audience
· Undergraduate students
· Postgraduate students
· Researchers and laboratory trainees in: Genetics, Molecular Biology, Biochemistry. Biotechnology and Microbiology.
Location -NRC
Prf. Dr. Kamal M. A. Khalil, Applied Microbial Genetics Lab., (Lab., 406–408), Genetics & Cytology Department, Biotechnology Research Institute, National Research Centre. 33 El Bohouth St., El Dokki, Giza, Egypt
I - DNA Extraction & Purification
II- DNA Separation & Quantification
Day 4
III - PCR Amplification of 16S rRNA gene
Day 1
- DNA extraction and purification from:
- DNA Separation. (Agarose Gel Electrophoresis)
- Measuring DNA concentration and purity (NanoDrop Spectrophotometer).
Day 2
- DNA Separation. (Agarose Gel Electrophoresis)
- Measuring DNA concentration and purity (NanoDrop Spectrophotometer).
Day 3
- DNA Separation. (Agarose Gel Electrophoresis)
- Measuring DNA concentration and purity (NanoDrop Spectrophotometer).
Day 4
- PCR Amplification of the 16S rRNA gene. 16S rRNA gene analysis for microbial identification and classification. (PCR) and (Agarose Gel Electrophoresis)
- DNA Separation. (Agarose Gel Electrophoresis)