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I made a video-rate laser scanning confocal and multi-photon microscopy system in In Vivo Micro-Visualization Laboratory in KAIST that was built on previously reported design (1-2).
(1) Veilleux I et al., IEEE Journal of Selected Topics in Quantum Electronics 14(1), 10-18, 2008
(2) Kim P et al., Journal of biomedical optics 13(1), 010501, 2008
Video-rate Laser Scanning Confocal Microscopy System
Video-rate Laser Scanning Confocal Microscopy System
- 30 frames (512 x 512 pixels) per second, 3 color-fluorescence detection (GFP, RFP, Far Red)
- Scanner consists of an aluminum-coated polygonal mirror with 36 facets (X-axis fast scan) and a silver-coated galvano mirror (Y-axis slow scan)
Details of the system were described in following papers.
Video-rate Laser Scanning Two-photon Microscopy System
Video-rate Laser Scanning Two-photon Microscopy System
- 30 frames (512 x 512 pixels) per second, 3 color-fluorescence (CFP, GFP/YFP, RFP) and SHG detection
- Scanner consists of an gold-coated polygonal mirror with 36 facets (X-axis fast scan) and a silver-coated galvano mirror (Y-axis slow scan)
- Laser power can be controlled by half-wave plate and polarizer
- A prism pair is adapted to the system for dispersion compensation.
Prototypes of Intravital Microscopy System
Prototypes of Intravital Microscopy System
I designed and implemented the compact version of the video-rate laser scanning confocal microscope.
1st Prototype
1st Prototype
Compact design for minimizing space
Video-rate laser scanning confocal microscopy system
- 30 frames (512 x 512 pixels) per second
- 3 color- fluorescence detection (GFP, RFP, Far Red)
- 3 color fiber laser (488, 561, 640 nm)
Funding: Global Frontier Project (Korea National Research Foundation)
2nd Prototype
2nd Prototype
Video-rate laser scanning confocal microscopy system
- 30 frames (512 x 512 pixels) per second
- 4 color- fluorescence detection (Violet, GFP, RFP, Far Red)
- 4 color fiber laser (405, 488, 561, 640 nm)
- Motorized pinholes
- XYZ translational animal stage with body temperature controller
- Combined design of optional two-photon microscope system
Funding: KAIST End Run Project
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