Research Methods:

This section provides an overview of the experimental approaches and model systems Dr. Khaja Shameem employs to investigate the molecular mechanisms of cardiovascular disease. His research integrates advanced cellular and molecular techniques with preclinical models of cardiac injury and repair. By combining these methodologies, he aims to uncover novel insights into signaling pathways, disease progression, and potential therapeutic strategies in heart disease.

Selected Publications:

Mohammed Abdul KS, Han K, Guerrero AB, Wilson CN, Kulkarni A, Purcell NH. Increased PHLPP1 expression through ERK-4E-BP1 signaling axis drives nicotine induced oxidative stress related damage of cardiomyocytes. J Mol Cell Cardiol. 2024 Aug;193:100-112. doi: 10.1016/j.yjmcc.2024.05.014. Epub 2024 Jun 6. PubMed PMID: 38851627.

Mohammed Abdul KS, Rayadurgam J, Faiz N, Jovanović A, Tan W. Cardioprotection by isosteviol derivate JC105: A unique drug property to activate ERK1/2 only when cells are exposed to hypoxia-reoxygenation. J Cell Mol Med. 2020 Sep;24(18):10924-10934. doi: 10.1111/jcmm.15721. Epub 2020 Aug 14. PubMed PMID: 32794652; PubMed Central PMCID: PMC7521240.

Neonatal Rat Ventricular Myocytes (NRVMs) for In vitro studies:

Selected Publications:

Mohammed Abdul KS, Han K, Guerrero AB, Wilson CN, Kulkarni A, Purcell NH. Increased PHLPP1 expression through ERK-4E-BP1 signaling axis drives nicotine induced oxidative stress related damage of cardiomyocytes. J Mol Cell Cardiol. 2024 Aug;193:100-112. doi: 10.1016/j.yjmcc.2024.05.014. Epub 2024 Jun 6. PubMed PMID: 38851627.

Selected Publications:

Mohammed Abdul KS, Han K, Guerrero AB, Wilson CN, Kulkarni A, Purcell NH. Increased PHLPP1 expression through ERK-4E-BP1 signaling axis drives nicotine induced oxidative stress related damage of cardiomyocytes. J Mol Cell Cardiol. 2024 Aug;193:100-112. doi: 10.1016/j.yjmcc.2024.05.014. Epub 2024 Jun 6. PubMed PMID: 38851627.

Mouse Model of TAC and Drug Induced Cardiac Hypertrophy:

Mouse Hemorrhagic Stroke Model:

Mouse Ischemic Stroke Model:

Hypoxia studies:

Selected Publications:

Mohammed Abdul KS, Faiz N, Jovanović A, Tan W. Isosteviol Protects H9c2 Cells Against Hypoxia-reoxygenation by Activating ERK1/2. Cardiovasc Hematol Disord Drug Targets. 2021;21(1):73-77. doi: 10.2174/1871529X21666210216122022. PubMed PMID: 33593268.

Mohammed Abdul KS, Rayadurgam J, Faiz N, Jovanović A, Tan W. Cardioprotection by isosteviol derivate JC105: A unique drug property to activate ERK1/2 only when cells are exposed to hypoxia-reoxygenation. J Cell Mol Med. 2020 Sep;24(18):10924-10934. doi: 10.1111/jcmm.15721. Epub 2020 Aug 14. PubMed PMID: 32794652; PubMed Central PMCID: PMC7521240.

Selected Publications:

Mohammed Abdul KS, Jovanović S, Jovanović A. Exposure to 15% oxygen in vivo up-regulates cardioprotective SUR2A without affecting ERK1/2 and AKT: a crucial role for AMPK. J Cell Mol Med. 2017 Jul;21(7):1342-1350. doi: 10.1111/jcmm.13064. Epub 2017 Jan 25. PubMed PMID: 28121062; PubMed Central PMCID: PMC5487919.

Mohammed Abdul KS, Jovanović S, Du Q, Sukhodub A, Jovanović A. Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH. Biochim Biophys Acta. 2015 May;1852(5):709-19. doi: 10.1016/j.bbadis.2015.01.001. Epub 2015 Jan 8. PubMed PMID: 25576887; PubMed Central PMCID: PMC4547089.

Mohammed Abdul KS, Jovanović S, Du Q, Sukhodub A, Jovanović A. A link between ATP and SUR2A: A novel mechanism explaining cardioprotection at high altitude. Int J Cardiol.2015;189:73-6. doi: 10.1016/j.ijcard.2015.04.069. Epub 2015 Apr 11. Review. PubMed PMID: 25885875; PubMed Central PMCID: PMC4461008.

Mohammed Abdul KS, Jovanović S, Sukhodub A, Du Q, Jovanović A. Upregulation of cardioprotective SUR2A by sub-hypoxic drop in oxygen. Biochim Biophys Acta. 2014 Nov;1843(11):2424-31. doi: 10.1016/j.bbamcr.2014.07.007. Epub 2014 Jul 23. PubMed PMID: 25064694; PubMed Central PMCID: PMC4331663.