ACE LAB

Animal Cell Engineering Lab

Department of Biological Sciences, KAISTProf. Gyun Min Lee

Welcome to Animal Cell Engineering Lab!

For the production of recombinant proteins, Chinese hamster ovary (CHO) cells have been the most popular hosts in industry since the first product, human tPA, was approved for marketing in the mid 1980's. As of 2002, more than 50 recombinant proteins produced from CHO cells are approved for marketing. Our research group focus on the research and development of therapeutic protein production using recombinant CHO cells. The topics range from molecular biology to bioprocess engineering.

  1. "Selective Endocytosis Recombinant Human BMPs Through Cell Surface Heparan Sulfate Proteoglycans in CHO Cells: BMP-2 and BMP-7", M. G. Kim, C. L. Kim, Y. S. Kim, J. W. Jang, and G. M. Lee, Sci. Rep. (2021).

  2. “Comprehensive Characterization of Dihydrofolate Reductase-Mediated Gene Amplification for the Establishment of Recombinant Human Embryonic Kidney 293 Cells Producing Monoclonal Antibodies”, S. Y. Lee, M. Baek, and G. M. Lee, Biotechnol. J. (2021).

  3. "Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system", S. H. Park, J. H. Park, J. H. Lee, H. M. Lee, Y. J. Kang, E. J. Lee, S. Shin, G. M. Lee and Y. G. Kim, Appl. Microbiol. Biotechnol. (2020).