What we do

Workflow of 19 F NMR–based serum amino acid profiling and data analysis. Serum samples collected from CRC patients were subjected to fluorine-labeling–based amino acid detection followed by 19 F NMR spectroscopy. Quantified amino acid data were processed using ratio-based normalization and multivariate analyses to characterize stage-specific metabolic patterns and construct machine learning models for prognostic risk prediction.

 Lee, J. Y., Kim, J., Yoon, T., Kwon, S. H., Park, S. C., Chun, D., Kim, D., Park, E. J., Kim, H., Lee, J. M. (2026) Circulating Amino Acid Network Remodeling Reveals Systemic Metabolic Reprogramming Predictive of Colorectal Cancer Recurrence and Metastasis, Advanced Science, 13(48), e76944 

 A New Therapeutic Strategy and Target Validation Based on the Discovery That Tumors Co-opt Gene Regulatory Programs Already Present in Normal Vascular Differentiation to Grow Blood Vessels 

Lee, A. J.#, Min, S.#, Park. S. C.#, Qiu, M. Y., Bae, J. M., Jin, S. Y., Choi, B., Park, J., Kwon, S. H., Kang, G. H., Lee, C. H., Yang, M. J., Park, J., Koh, G. Y.*, Lee, J. M.*, Jung, I.* (2026) A Co-opted Developmental Gene Regulatory Program in Endothelial Progenitors Promotes Tumor Angiogenic Phenotypes, Cancer Research, 86(17), 4381-4399 (# : Equal contribution, *: Co-correspondence). 

Various different molecular mechanisms for PTMs changing a protein’s proteolytic stability have been found. DTL (for Denticleless protein homolog, also called CDT2) is the human orthologue of yeast Sic1 and itself a substrate adapter of the DCX E3 ligase complex. DTL is phosphorylated at T464, which promotes interaction with 14-3-3, thus shielding it from degradation by SCFFBXO11

Lee, J. M., Hammarén, H.M., Savitski, M.M., and Baek, S.H. (2023) Control of Protein Stability by Posttranslational Modifications. Nature Communications, 14 (1), 201 DOI: 10.1038/s41467-023-35795-8 

mRNA bridge mimetics for the programmed cleavage by Ago-catalyzed miRNA-mediated mRNA decay and its application to control nucleus translocation of CRISPR Self Check-In. (A) Schematic representation of CRISPR Self Check-In operation for controlled genome editing. In disease cells, CRISPR Self Check-In controls nucleus translocation of Cas9 proteins for disease-specific genome editing by sensing miRNAs and being cleaved off mRNA bridge mimetics containing NES.  

Shin, C-H.#, Park, S. C.#, Park, I-G., Kim, H., Lee, C., Kim, S-H., Lee, J.*, Lee, J. M.*, Oh, S. J.* (2022) Cytosolic microRNA-inducible nuclear translocation of Cas9 protein for disease-specific genome modification Nucleic Acids Research  50 (10), 5919-5933 (# : Equal contribution, *: Co-correspondence).