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In Chamaerops humilis, volatiles are emitted by leaves, but there is no specific cells. Here is a staining of terpenes by NaDi reagent in the upper epidermis and in the mesophyll.
Neutral Red also stains the epidermis of Chamaerops humilis. It could indicates a high level of secretion of volatiles.
Here is a simple view of an hand-made section of a Chamaerops humilis palm stained with Rawlins-Takahashi staining.
In Chamaerops humilis idioblasts can be detected with specific stains. There function is not known. Here are two views with Lugol staining. Strange spherical structures can be seen inside cells.
In Brassica juncea, extrafloral nectaries are spray all over the stems. We have discovered them in 2013. Here are two views: 1/transversal and-made section; 2/top-view by environmental microscopy
In Arabidopsis lyrata, terpene droplets can be seen in the conical cells of the petal epidermis. Here, terpenes are stained by the NaDi reagent.
In Trollius europaeus, there are unicellular trichomes on the lower epidermis of tepals. These trichomes secrete an oily substance. Here is a staining of this oil by Nile Red.
In Moss Roses, there is a reiteration of the development of trichomes on trichomes.
When expressed in Nicotiana tabacum, the specific AtGL1 promoter of unicellular trichomes, is expressed in secreting trichomes. Here, the AtGL1 promoter is fused to GFP (green, GFP; red, chlorophyll).
In Nicotiana sylvestris, there are secreting and non secreting trichomes. In this mutant, trichomes are distorted. Here is a view by environmental microscopy.
In Mentha spicata, peltate trichomes have 8 headcells. In this mutant, they have 9 to 12 headcells. Here, nucleus are stained with DAPI.
In Mentha x piperita, flower buds are covered with glandular trichomes for direct defence (false colors in Scanning Electron Microscopy)
In Mentha x piperita, the ATP promoter of Arabidopsis thaliana fused to the GUS reporter gene, is expressed in both peltate and capitate glandular trichomes.
In Salvia sclarea, numerous intracellular vesicles can be stained by Floral Yellow in the neck- and head-cells of glandular trichomes. They could be essential oil droplets.
In Salvia sclarea, imaging of sclareol cristals is possible with Second Harmonic Generation Microscopy (in green).
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