Key Technologies

Measurement of modular kinetics in isolated mitochondria by simultaneous polarographic, potentiometric and fluorescent measurements

Measurement of oxygen consumption (mitochondrial activity) and extracellular acidification (glycolysis) using the extracellular flux analyzer (Seahorse Bioscience)

Mouse metabolic phenotyping

Measurement of mitochondrial reactive oxygen species in isolated mitochondria (using hydrogenperoxide sensitive probes) and intact cells (using superoxide sensitive probes)

Measurement of plasma and mitochondrial membrane potential in intact cells (using time-lapse fluorescence microscopy and platereader-based kinetic measurements)