Week 1 - Genomics

Day 1 - Introduction to the workshop and field of Bioinformatics

Bioinformatics Introduction and applications

Biological databases – Introduction to Genomic and Proteomic databases

Day 2 - Sequence Analysis

Sequence Analysis – Pairwise alignment - BLAST

Multiple Sequence Alignment

Day 3 - Introduction to NGS Data

Sequencing technologies and terminologies

Next Generation Sequencing Data Analysis overview - Variant Analysis approach

Day 4 - RNA-seq data analysis

RNA Seq for Differential Gene Expression

Demonstration of RNA Seq data analysis using Galaxy

Day 5 - Enrichment analysis

Functional enrichment analysis of the Differentially expressed genes - Ontologies and Pathways

Demonstration using Galaxy

Week 2 - Structural Bioinformatics

Day 1 - Introduction to Structural Bioinformatics

A brief introduction to biomolecular structure data, databases, informatics methods and applications in biology

Day 2 - Biomolecular Structure Visualization (UCSF Chimera and Discovery Studio)

Visualization of biomolecules in various states to understand life processes, unearth mechanisms of biological reactions.

Visualizing structure data to understand quality of the structures

Day 3 - Structure alignment and comparisons (UCSF Chimera and Discovery Studio)

Methods for comparing protein structures

Methods for comparing poses of different structures

Day 4 - Molecular Modelling (UCSF Chimera and Modeller)

Fundamentals of molecular modelling

Protein structure prediction (modelling) using computational methods

Day 5 - Molecular Docking (UCSF Chimera, AutoDock Vina and ClusPro)

Understanding process of molecular recognition and interactions

Molecular docking as a tools to investigate biomolecular interactions