The Schedule

Day 1 Evening, Session 1

Zoom recordings: LINK

Welcome and announcements. Ido Braslavsky & Peter Davies (10 min)

Events at the ice surface (part 1) Ran Drori chairing

Ran Drori Opening remarks (10+5 min)

Julian Gerhaeuser Correlation between the ice surface structure and the Gibbs-Thomson equation in Molecular Dynamics Simulations (15+5 min)

Amish Patel Molecular Insights into the Interactions between Antifreeze Proteins and Ice (20+10 min)

Kenji_Mochizuki Melt growth mechanism of basal ice face and its link to AFPs (10+5 min)

BREAK 10 min

Hossam Farag Free Energy Barriers for Anti-Freeze Protein Engulfment: A Model for the Effects of Supercooling, Footprint Size, and Spatial Separation (20+10 min)

A. Malinowska Local curvature analysis of ice recrystallisation inhibition (10+5 min)

Jeongmoon Choi Identifying Ice-Philic Patches to Inform the Ice Binding Sites of Antifreeze Proteins (10+5 min)

GENERAL DISCUSSION 15 min

Day 2 Morning Session 2

Zoom recordings: LINK

Biological ice nucleation Valeria Molinero chairing

Valeria Molinero Opening remarks (15 min)

Tina Šantl-Temkiv Structure and Protein-Protein Interactions of Ice Nucleation Proteins Drive Their Activity (30+10 min)

Jocelyn Lee Probing the structure-function relationships of a bacterial ice nucleation protein guided by its AlphaFold model (10+5 min)

Thomas Hansen Investigating the higher order structure of a bacterial ice nucleation protein (15+5 min)

BREAK 10 min

Virginia Walker Purple brome downs the champion, Pseudomonas syringae, with a remarkable “jab-cross-hook” combination (20+10 min)

Ingrid Ribeiro Tetramer is the minimum size of the INP aggregate in P. syringae (20+10 min)

GENERAL DISCUSSION 15 min

Day 2 Evening Session 3

IBP Nomenclature, Discovery, and Evolution Chris Cheng chairing.

Zoom recordings: LINK

Chris Cheng Opening remarks (20 min)

Peter Davies Nomenclature of IBPs (15+5 min)

Onnela Luhila Ice-binding proteins in psychrophilic bacteria – isolation, characterization, and applications in food (10+5 min)

Johanna Winder The diversity of ice-binding proteins in the polar diatom Fragilariopsis cylindrus (10+5 min)

BREAK 10 min

Rob Eves Using small IBPs as affinity tags in protein purification (10+5 min)

Joanna Kelley Evolution of type III antifreeze proteins in Zoarcoidei (15+5 min)

Laurie Graham Origins of type I AFPs (20+10 min)

GENERAL DISCUSSION 15 min

Day 3 Morning Session 4

Events at the ice surface (part 2) Ran Drori chairing

Zoom recordings: LINK

Ido Braslavsky Atomic force microscopy for the investigation of ice surfaces covered by IBPs (20+10 min)

Roderick Tas Revealing the interfacial interactions of single, ice-bound antifreeze proteins through subzero nanoscopy (20+10 min)

Tolou Shokuhfar Real-time, nanoscale observation of ice nucleation on TiO2 nanoparticles in water (20+10 min)

BREAK 10 min

Structure-function relationships in IBPs Chair TBA

Sakae Tsuda Fish ice-binding proteins differently function at higher concentrations (15+5 min)

Douglas Laurents NMR Studies of the Snow Flea Antifreeze Protein as a Model Polyproline II Helical Bundle to Advance Protein Design (15+5 min)

Connor Scholl Exploring the activity-size relationship in IBPs through protein engineering (10+5 min)

Biman Jana Connecting cold denaturation and antifreeze proteins: uncovering nature’s design (20+10 min)

GENERAL DISCUSSION 15 min

Day 3 Evening Session 5

Applications of IBPs 1 Sakae Tsuda chairing

Zoom recordings: LINK

Sakae Tsuda Opening remarks (10+5 min)

Jianjun Wang Revealing the mechanism of IBPs for cryopreservation (20+10 min)

Tatsuya Arai Elucidating the molecular mechanism of cell protective function of ice-binding proteins at non-freezing temperature (10+5 min)

Masahiro Kuramochi Expression of Ice-Binding Proteins in Caenorhabditis elegans Improves the Survival Rate after freezing and cold shock (10+5 min)

BREAK 10 min

Katrin Bamberg Winter flounder meets Gila Monster - Structure and Activity of (photoswitchable) AFP-Tc-Protein Chimera (10+5 min)

Gang Ren Macromolecular structures in ice (15+5 min)

Tim Hogervorst Ice-binding with nano-tube forming cyclic peptides (10+5 min)

Ido Braslavsky Fortified ice - cellulose composite by ice-binding protein and cellulose-binding protein chimera. (10+5 min)

Day 4 Morning Session 6

Jack Duman Lifetime Achievement Award (Introduction by Laurie Graham)

Zoom recordings: LINK

Applications of IBPs 2 Ido Braslavsky chairing

Prakriti Kashyap Isolation of ice-binding proteins from Himalayan barley (10+5 min)

Daniëlle van den Broek Ice-binding proteins for cryopreservation of heart cells and tissue (10+5 min)

Krisztina Varga An insect antifreeze protein from Anatolica polita: structure and its applications in cryoprotection of mammalian cells (15+5 min)

Ana Marie Lyons Comparative proteomics of cold-treated tardigrades reveals shifts in key biological processes and identifies putative ice-binding proteins (10+5 min)

BREAK 10 min

Yusuke Kato Antifreeze Properties of Antifreeze Protein-Poly(vinyl alcohol) Conjugates (10+5 min)

Matthew Reeder Inhibiting Ice Recrystallization by Corn Cob Hemicelluloses (10+5 min)

John Tsavalas Ice Nucleation Promotion Impact on Ice Recrystallization Inhibition Activity of Polyols (15+5 min)

Closing remarks