2023.7-2024.2
NSTC Undergraduate Research Project (Shiang Lin)- Use of point mutation to study the function of photosystem I subunit in cyanobacteria under far-red light
2019.8-2024.7
MOST Grant for the Einstein Program (PI)
Studying the environmental impact, the mechanism, and the application of using far-red light for oxygenic photosynthesis
2020.7-2021.2
MOST Undergraduate Research Project (Ting-Shuo Nien)- To the ecological niche of far-red light using cyanobacteria
We are studying why cyanobacteria can use far-red light for oxygenic photosynthesis, how important they are, and where they are in the environment. Understanding this mechanism benefits re-evaluating global primary production and enhancing photosynthetic efficiency in plants and algae. This mechanism can also be applied to synthetic biology in controlling the syntheses of desired products.
Our current research topics are: (1) Investigating the role of cyanobacteria and algae using far-red light for photosynthesis in the environment, (2) Elucidating the mechanism of photosynthesis in far-red light and other special conditions, (3) the application of cyanobacteria in synthetic biology.
Our approaches include sampling in the environment, 16S rRNA sequencing, isolation of strains, spectroscopy, pigment analysis, HPLC, oxygen electrode, bacterial genetics, gene function and regulation, and characterization of protein complexes.
Photosynthetic machinery in cyanobacteria
Chlorophyll (Chl) d and Chl f are the two Chls that can harvest far-red light (FRL, wavelength = 700-800 nm)
Some cyanobacteria with this specific gene cluster can perform far-red light photoacclimation (FaRLiP). This process includes syntheses of Chl d and Chl f and remodeling of photosystem I (PSI), photosystem II (PSII), and phycobilisome (PBS). These modifications allow those cyanobacteria to harvest FRL for photosynthesis.
The regulatory pathway of far-red light photoacclimation (FaRLiP) 遠紅光轉換的調控機制
Engineering the capability of using FRL in plants and algae can potentially increase their photosynthetic productivity.
Plants, algae, and cyanobacteria can harvest more light if they can use both visible light and far-red light for photosynthesis.