Embedded Files
Water dipping epifluorescence
Water dipping epifluorescence
Zeiss Axiolab A1 with three colour fluorescence; regular objectives and x10 and x40 'dipping' objectives. All coupled to a Ximea 12 Mbp camera for, sensitive fluorescence live imaging
Zeiss Axiolab A1 with three colour fluorescence; regular objectives and x10 and x40 'dipping' objectives. All coupled to a Ximea 12 Mbp camera for, sensitive fluorescence live imaging
Intracellular Calcium
Intracellular Calcium
We use the genetically encoded calcium indicator GCaMP6 developed at Janelia Farm by Tsai-Wen Chen to explore signaling events in nephrocytes an cardiomyocytes
We use the genetically encoded calcium indicator GCaMP6 developed at Janelia Farm by Tsai-Wen Chen to explore signaling events in nephrocytes an cardiomyocytes
Reagents
Reagents
We have developed and validated polyclonal antisera to nephrocyte proteins (dKlf15, sns and duf). We also have dKlf15-Gal4 and UAS-dKlf15 fly lines. Happy to share!
We have developed and validated polyclonal antisera to nephrocyte proteins (dKlf15, sns and duf). We also have dKlf15-Gal4 and UAS-dKlf15 fly lines. Happy to share!
Behavioural analysis
Behavioural analysis
The lab has used Drosophila Activity Monitoring Systems (DAMS) from Trikinetics to study the relationship between metabolism and sleep. These are simple systems and good for undergraduate projects.
The lab has used Drosophila Activity Monitoring Systems (DAMS) from Trikinetics to study the relationship between metabolism and sleep. These are simple systems and good for undergraduate projects.
Imaging
Imaging
The lab maintains 'Hidden Worlds' a small selection of microscopy images showcasing different imaging outputs from both confocal and widefield microscopes.
The lab maintains 'Hidden Worlds' a small selection of microscopy images showcasing different imaging outputs from both confocal and widefield microscopes.
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