Publications

Publications (Kenichi Nagase)

Cover Art Gallery (Kenichi Nagase)

Publications (Kenichi Nagase)

Nakao M, Nagase K*, Harvesting methods of umbilical cord-derived mesenchymal stem cells from culture modulate cell properties and functions, Regenerative Therapy, 26, 80-88, (2024) DOI: 10.1016/j.reth.2024.05.010
Nagase K*, Nagaoka M, Nakano Y, Utoh R, bFGF-releasing biodegradable nanoparticles for effectively engrafting transplanted hepatocyte sheet. Journal of Controlled Release, 366, 160-169, (2024) DOI: 10.1016/j.jconrel.2023.12.040
Nagase K*, Nagaoka M, Matsuda J, Kojima N, Thermoresponsive block-copolymer brush-modified interfaces for effective fabrication of hepatocyte sheets, Materials & Design, 239, 112824, (2024) DOI: 10.1016/j.matdes.2024.112824
Nagase K*, Bioanalytical technologies using temperature-responsive polymers, Analytical Sciences 40, 5, 827-841

(2024) DOI: 10.1007/s44211-024-00545-3 (Hot Article)

Hirotani T, Nagase K*, Temperature-modulated separation of vascular cells using thermoresponsive-anionic block copolymer-modified glass, Regenerative Therapy, 27, 259-267, (2024) DOI: 10.1016/j.reth.2024.03.009
Nakao M, Matsui M, Kim K, Nishiyama N, Grainger DW, Okano T, Kanazawa H, Nagase K*, Umbilical cord-derived mesenchymal stem cell sheets transplanted subcutaneously enhance cell retention and survival more than dissociated stem cell injections, Stem Cell Research & Therapy 14, 1, 352 (2023) DOI: 10.1186/s13287-023-03593-0
Nagase K*, Shimane R, Ethylene glycol-based thermoresponsive block copolymer brushes with cell-affinity peptides for thermally controlled interaction with target cells, Materials & Design, 233, 112234, (2023) DOI: 10.1016/j.matdes.2023.112234.
Nagase K*, Matsuda J, Takeuchi A, Ikemoto Y, Hydration and dehydration behaviors of poly(N-isopropylacrylamide)-grafted silica beads, Surface and Interfaces, 40, 103058, (2023) DOI: 10.1016/j.surfin.2023.103058.
Nagase K*, Wakayama H, Matsuda J, Kojima N, Kanazawa H, Thermoresponsive mixed polymer brush to effectively control the adhesion and separation of stem cells by altering temperature, Materials Today Bio, 20, 100627, (2023), DOI: 10.1016/j.mtbio.2023.100627
Nagase K*, Yamazaki K, Maekawa Y, Kanazawa H, Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes, Materials Today Bio, 18, 100521, (2023), DOI: 10.1016/j.mtbio.2022.100521
Takahashi H, Wakayama H, Nagase K, Shimizu T, Engineered human muscle tissue from multilayered aligned myofiber sheets for studies of muscle physiology and predicting drug response, Small Methods, 7, 2200849, (2023), DOI：10.1002/smtd.202200849, (Inside Front Cover)
Nagase K*, Okada A, Matsuda J, Ichikawa D, Hattori Y, Kanazawa H, A thermoresponsive cationic block copolymer brush-grafted silica bead interface for temperature-modulated separation of adipose-derived stem cells, Colloids and Surfaces B: Biointerfaces, 220, 112928 (2022), DOI: 10.1016/j.colsurfb.2022.112928.
Nagase K*, Kojima N, Goto M, Akaike T, Kanazawa H. Thermoresponsive block copolymer brush for temperature-modulated hepatocyte separation. Journal of Materials Chemistry B, 10(42), 8629-8641 (2022), DOI: 10.1039/D2TB01384C (Front Cover Article).
Nagase K*, Ishii S, Takeuchi A, Kanazawa H. Temperature-modulated antibody drug separation using thermoresponsive mixed polymer brush-modified stationary phase. Separation and Purification Technology, 299, 121750 (2022), DOI: 10.1016/j. seppur.2022.121750.
Nagase K*, Takagi H, Nakada H, Ishikawa H, Nagata Y, Aomori T, Kanazawa H, Chromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring, Scientific Reports, 12(1), 12847 (2022), DOI: 10.1038/s41598-022-16928-3.
Nagase K*, Kitazawa S, Kogure T, Yamada S, Katayama K, Kanazawa H. Viral vector purification with thermoresponsive-anionic mixed polymer brush modified beads-packed column. Separation and Purification Technology 286, 120445 (2022), DOI:10.1016/j.seppur.2022.120445
Nagase K*, Matsumoto K, Kanazawa H, Temperature-responsive mixed-mode column for the modulation of multiple interactions, Scientific Reports, 12(1), 4434, (2022), DOI: 10.1038/s41598-022-08475-8.
Nagase K*, Inoue S, Inoue M, Kanazawa H, Two-dimensional temperature-responsive chromatography using a poly(N-isopropylacrylamide) brush-modified stationary phase for effective therapeutic drug monitoring, Scientific Reports, 12(1), 2653 (2022), DOI: 10.1038/s41598-022-06638-1.
Nagase K*, Nishiyama T, Inoue M, Kanazawa H, Temperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase, Scientific Reports, 11(1), 23508, DOI: 10.1038/s41598-021-02998-2 (2021).
Nagase K*, Edatsune G, Nagata Y, Matsuda J, Ichikawa D, Yamada S., Hattori Y, Kanazawa H, Thermally-modulated cell separation columns using a thermoresponsive block copolymer brush as a packing material for the purification of mesenchymal stem cells, Biomaterials Science, 9(21), 7054-7064, (2021), DOI: 10.1039/D1BM00708D (Front Cover Article).
Nagase K*, Thermoresponsive interfaces obtained using poly(N-isopropylacrylamide)-based copolymer for bioseparation and tissue engineering applications, Advances in Colloid and Interface Science, 295, 102487 (2021), DOI: 10.1016/j.cis.2021.102487.
Nagase K*, Ishizawa Y, Inoue M, Kokubun M, Yamada S, Kanazawa H, Temperature-responsive spin column for sample preparation using an all-aqueous eluent, Analytica Chimica Acta, 1179, 338806, (2021), DOI: 10.1016/j.aca.2021.338806.
Nagase K*, Umemoto Y, Kanazawa H, Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns, Scientific Reports, 11(1), 9976, (2021), DOI: 10.1038/s41598-021-89165-9.
Homma K, Ohta Y, Minami K, Yoshikawa G, Nagase K, Akimoto A M, Yoshida R, Autonomous Nanoscale Chemomechanical Oscillation on the Self-Oscillating Polymer Brush Surface by Precise Control of Graft Density, Langmuir, 37(14), 4380-4386, (2021), DOI: 10.1021/acs.langmuir.1c00459.
Nagase K*, Shimura M, Shimane R, Hanaya K, Yamada S, Akimoto A M, Sugai T, Kanazawa H, Selective capture and non-invasive release of cells using a thermoresponsive polymer brush with affinity peptides, Biomaterials Science, 9(3), 663-674, (2021), DOI: 10.1039/D0BM01453B (Front Cover Article).
Nomoto D, Nagase K, Nakamura Y, Kanazawa H, Citterio D, Hiruta Y, Anion species-triggered antibody separation system utilizing a thermo-responsive polymer column under optimized constant temperature, Colloids and Surfaces B: Biointerfaces, 205, 111890, (2021), DOI: 10.1016/j.colsurfb.2021.111890.
Maekawa Y, Ayano E, Nagase K, Kanazawa H, Effective Separation for New Therapeutic Modalities Utilizing Temperature-Responsive Chromatography, Analytical Sciences, 37, 651-660, (2021), DOI: 10.2116/analsci.20SCR09.
Nagase K*, Uchikawa N, Hirotani T, Akimoto A M, Kanazawa H, Thermoresponsive anionic copolymer brush-grafted surfaces for cell separation, Colloids and Surfaces B: Biointerfaces, 185, 110565, (2020), DOI: 10.1016/j.colsurfb.2019.110565.
Nagase K*, Kitazawa S, Yamada S, Akimoto A M, Kanazawa H, Mixed polymer brush as a functional ligand of silica beads for temperature-modulated hydrophobic and electrostatic interactions, Analytica Chimica Acta, 1095, 1-13, (2020), DOI: 10.1016/j.aca.2019.10.058, (Front Cover Article).
Nishimoto T, Matsukawa K, Nagase K, Kanazawa H, Akimoto A M, Yoshida R, Design of two complementary copolymers that work as a glue for cell-laden collagen gels, Chemical Communications, 56, 10545-10548, (2020), DOI: 10.1039/D0CC03689G.
Nagase K*, Shukuwa R, Takahashi H, Takeda N, Okano T, Enhanced mechanical properties and cell separation with thermal control of PIPAAm-brushed polymer-blend microfibers, Journal of Materials Chemistry B, 8, 6017-6026, (2020), DOI: 10.1039/D0TB00972E (Front Cover Article).
Maekawa Y, Okamoto N, Okada Y, Nagase K, Kanazawa H, Green analytical method for the simultaneous analysis of cytochrome P450 probe substrates by poly(N-isopropylacrylamide)-based temperature-responsive chromatography, Scientific Reports, 10(1), 8828, (2020), DOI: 10.1038/s41598-020-65270-z.
Nagase K*, Ota A, Hirotani T, Yamada S, Akimoto A M, Kanazawa H, Thermoresponsive Cationic Block Copolymer Brushes for Temperature-Modulated Stem Cell Separation, Macromolecular Rapid Communications, 41(19), 2000308, (2020), DOI: 10.1002/marc.202000308 (Front Cover Article).
Maekawa Y, Yamazaki K, Ihara M, Nagase K, Kanazawa H, Simultaneous analysis of multiple oligonucleotides by temperature-responsive chromatography using a poly(N-isopropylacrylamide)-based stationary phase, Analytical and Bioanalytical Chemistry, 412, 5341-5351, (2020), DOI: 10.1007/s00216-020-02749-8.
Nagase K*, Ishii S, Ikeda K, Yamada S, Ichikawa D, Akimoto A, Hattori Y, Kanazawa H, Antibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions., Scientific Reports, 10, 11896, (2020), DOI: 10.1038/s41598-020-68707-7.
Nagase K*, Kanazawa H, Temperature-responsive chromatography for bioseparations, Analytica Chimica Acta, 1138, 191-212, (2020), DOI: 10.1016/j.aca.2020.07.075 (Front Cover Article).
Nagase K*, Watanabe M, Zen F, Kanazawa H, Temperature-responsive mixed-mode column containing temperature-responsive polymer-modified beads and anionic polymer-modified beads, Analytica Chimica Acta, 1079, 220-229, (2019), DOI: 10.1016/j.aca.2019.06.010.
Nagase K*, Hasegawa M, Ayano E, Maitani Y, Kanazawa H, Effect of Polymer Phase Transition Behavior on Temperature-Responsive Polymer-Modified Liposomes for siRNA Transfection, International Journal of Molecular Sciences, 20(2), 430, (2019), DOI: 10.3390/ijms20020430.
Maekawa-Matsuura M, Fujieda K, Maekawa Y, Nishimura T, Nagase K*, Kanazawa H, LAT1-Targeting Thermoresponsive Liposomes for Effective Cellular Uptake by Cancer Cells, ACS Omega, 4(4), 6443-6451, (2019), DOI: 10.1021/acsomega.9b00216.
Nagase K*, Inanaga D, Ichikawa D, Mizutani Akimoto A, Hattori Y, Kanazawa H, Temperature-modulated cell-separation column using temperature-responsive cationic copolymer hydrogel-modified silica beads, Colloids and Surfaces B: Biointerfaces, 178, 253-262, (2019), DOI: 10.1016/j.colsurfb.2019.02.057 (Front Cover Article).
Nemoto R, Fujieda K, Hiruta Y, Hishida M, Ayano E, Maitani Y, Nagase K, Kanazawa H, Liposomes with temperature-responsive reversible surface properties, Colloids and Surfaces B: Biointerfaces, 176, 309-316, (2019), DOI: 10.1016/j.colsurfb.2019.01.007.
Nakao M, Kim K, Nagase K, Grainger D W, Kanazawa H, Okano T, Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets, Stem Cell Research & Therapy, 10(1), 353, (2019), DOI: 10.1186/s13287-019-1431-6.
Nakao M, Inanaga D, Nagase K*, Kanazawa H, Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins, Regenerative Therapy, 11, 34-40, (2019), DOI: 10.1016/j.reth.2019.01.002.
Li J, Kaku T, Tokura Y, Matsukawa K, Homma K, Nishimoto T, Hiruta Y, Akimoto A M, Nagase K, Kanazawa H, Shiratori S, Adsorption–Desorption Control of Fibronectin in Real Time at the Liquid/Polymer Interface on a Quartz Crystal Microbalance by Thermoresponsivity, Biomacromolecules, 20(4), 1748-1755, (2019), DOI: 10.1021/acs.biomac.9b00121.
Konishi T, Mizutani Akimoto A, Nishimoto T, Tokura Y, Tenjimbayashi M, Homma K, Matsukawa K, Kaku T, Hiruta Y, Nagase K, Kanazawa H, Shiratori S, Crosslinked Poly(N-Isopropylacrylamide)-Based Microfibers as Cell Manipulation Materials with Prompt Cell Detachment, Macromolecular Rapid Communications, 40(23), 1900464, (2019), DOI: 10.1002/marc.201900464.
Homma K, Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Stable and Prolonged Autonomous Oscillation in a Self-Oscillating Polymer Brush Prepared on a Porous Glass Substrate, Langmuir, 35(30), 9794-9801, (2019), DOI: 10.1021/acs.langmuir.9b00928.
Okubo K, Ikeda K, Oaku A, Hiruta Y, Nagase K*, Kanazawa H, Protein purification using solid-phase extraction on temperature-responsive hydrogel-modified silica beads, Journal of Chromatography A, 1568, 38-48, (2018), DOI: 10.1016/j.chroma.2018.07.027.
Nagase K*, Yamato M, Okano T, Temperature‐responsive Polymers for Tissue Engineering, Temperature‐Responsive Polymers: Chemistry, Properties and Applications, 301-312 (2018), DOI 10.1002/9781119157830.ch12.
Nagase K*, Yamato M, Kanazawa H, Okano T, Poly(N-isopropylacrylamide)-based thermoresponsive surfaces provide new types of biomedical applications, Biomaterials, 153, 27-48, (2018), DOI: 10.1016/j.biomaterials.2017.10.026.
Nagase K*, Okano T, Kanazawa H, Poly(N-isopropylacrylamide) based thermoresponsive polymer brushes for bioseparation, cellular tissue fabrication, and nano actuators, Nano-Structures & Nano-Objects, 16, 9-23, (2018), DOI: 10.1016/j.nanoso.2018.03.010.
Matsuura M, Ohshima M, Hiruta Y, Nishimura T, Nagase K*, Kanazawa H, LAT1-Targeting Thermoresponsive Fluorescent Polymer Probes for Cancer Cell Imaging, International Journal of Molecular Sciences, 19(6), 1646, (2018), DOI: 10.3390/ijms19061646.
Nagase K*, Okano T, Thermoresponsive Polymer Brushes for Thermally Modulated Cell Adhesion and Detachment, Polymer and Biopolymer Brushes: For Materials Science and Biotechnology, pp. 361-375 (2018), DOI 10.1002/9781119455042.ch12.
Masuda T, Akimoto A M, Furusawa M, Tamate R, Nagase K, Okano T, Yoshida R, Aspects of the Belousov–Zhabotinsky Reaction inside a Self-Oscillating Polymer Brush, Langmuir, 34(4), 1673-1680, (2018), DOI: 10.1021/acs.langmuir.7b03929.
Akimoto A, Niitsu E, Nagase K, Okano T, Kanazawa H, Yoshida R, Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation, International Journal of Molecular Sciences, 19(4), 1253, (2018), DOI: 10.3390/ijms19041253.
Masuda T, Kajisa T, Akimoto A M, Fujita A, Nagase K, Okano T, Sakata T, Yoshida R, Dynamic electrical behaviour of a thermoresponsive polymer in well-defined poly(N-isopropylacrylamide)-grafted semiconductor devices, RSC Advances, 7(55), 34517-34521, (2017), DOI: 10.1039/C7RA05786E.
Nagase K*, Shukuwa R, Onuma T, Yamato M, Takeda N, Okano T, Micro/nano-imprinted substrates grafted with a thermoresponsive polymer for thermally modulated cell separation, Journal of Materials Chemistry B, 5(30), 5924-5930, (2017), DOI: 10.1039/C7TB01251A (Front Cover Article).
Nagase K*, Sakurada Y, Onizuka S, Iwata T, Yamato M, Takeda N, Okano T, Thermoresponsive polymer-modified microfibers for cell separations, Acta Biomaterialia, 53, 81-92, (2017), DOI: 10.1016/j.actbio.2017.02.033.
Nagase K*, Nagumo Y, Kim M, Kim H-J, Kyung H-W, Chung H-J, Sekine H, Shimizu T, Kanazawa H, Okano T, Lee S-J, Yamato M, Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets, Macromolecular Bioscience, 17(8), 1700073, (2017), DOI: 10.1002/mabi.201700073, (Front Cover Article).
Homma K, Masuda T, Akimoto A M, Nagase K, Itoga K, Okano T, Yoshida R, Fabrication of Micropatterned Self-Oscillating Polymer Brush for Direction Control of Chemical Waves, Small, 13(21), 1700041, (2017), DOI: 10.1002/smll.201700041.
Nagase K*, Okano T, Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations, Journal of Materials Chemistry B, 4(39), 6381-6397, (2016), DOI: 10.1039/c6tb01003b.
Nagase K*, Okano T, Bioseparation Using Thermoresponsive Polymers, Encyclopedia of Biocolloid and Biointerface Science, 220-230 (2016), DOI 10.1002/9781119075691.ch16.
Nagase K*, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules, RSC Advances, 6(95), 93169-93179, (2016), DOI: 10.1039/c6ra20944k.
Nagase K*, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Protein separations via thermally responsive ionic block copolymer brush layers, RSC Advances, 6(31), 26254-26263, (2016), DOI: 10.1039/c6ra01061j.
Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Artificial cilia as autonomous nanoactuators: Design of a gradient self-oscillating polymer brush with controlled unidirectional motion, Science Advances, 2(8), e1600902, (2016), DOI: 10.1126/sciadv.1600902.
Akimoto A M, Hasuike E, Tada H, Nagase K, Okano T, Kanazawa H, Yoshida R, Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture, Analytical Sciences, 32(11), 1203-1205, (2016), DOI: 10.2116/analsci.32.1203.
Nagase K*, Onuma T, Yamato M, Takeda N, Okano T, Enhanced Wettability Changes by Synergistic Effect of Micro/Nanoimprinted Substrates and Grafted Thermoresponsive Polymer Brushes, Macromolecular Rapid Communications, 36(22), 1965-1970, (2015), DOI: 10.1002/marc.201500393, , (Front Cover Article).
Nagase K*, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation, RSC Advances, 5(81), 66155-66167, (2015), DOI: 10.1039/c5ra11038f.
Nagase K*, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Thermoresponsive Cationic Copolymer Brushes for Mesenchymal Stem Cell Separation, Biomacromolecules, 16(2), 532-540, (2015), DOI: 10.1021/bm501591s.
Masuda T, Terasaki A, Akimoto A M, Nagase K, Okano T, Yoshida R, Control of swelling-deswelling behavior of a self-oscillating gel by designing the chemical structure, RSC Advances, 5(8), 5781-5787, (2015), DOI: 10.1039/c4ra10675j.
Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R, Design of Self-Oscillating Polymer Brushes and Control of the Dynamic Behaviors, Chemistry of Materials, 27(21), 7395-7402, (2015), DOI: 10.1021/acs.chemmater.5b03228.
Nagase K*, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive Anionic Copolymer Brushes Containing Strong Acid Moieties for Effective Separation of Basic Biomolecules and Proteins, Biomacromolecules, 15(10), 3846-3858, (2014), DOI: 10.1021/bm5012163.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Monolithic Silica Rods Grafted with Thermoresponsive Anionic Polymer Brushes for High-Speed Separation of Basic Biomolecules and Peptides, Biomacromolecules, 15(4), 1204-1215, (2014), DOI: 10.1021/bm401779r.
Nagase K, Geven M, Kimura S, Kobayashi J, Kikuchi A, Akiyama Y, Grijpma D W, Kanazawa H, Okano T, Thermoresponsive Copolymer Brushes Possessing Quaternary Amine Groups for Strong Anion-Exchange Chromatographic Matrices, Biomacromolecules, 15(3), 1031-1043, (2014), DOI: 10.1021/bm401918a.
Matsuura K, Utoh R, Nagase K, Okano T, Cell sheet approach for tissue engineering and regenerative medicine, Journal of Controlled Release, 190(0), 228-239, (2014), DOI: 10.1016/j.jconrel.2014.05.024.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermally Modulated Cationic Copolymer Brush on Monolithic Silica Rods for High-Speed Separation of Acidic Biomolecules, ACS Applied Materials & Interfaces, 5(4), 1442-1452, (2013), DOI: 10.1021/am302889j.
Ayano E, Suzuki Y, Nishio T, Nagata Y, Kanazawa H, Nagase K, Okano T, Liquid Chromatography-Mass Spectrometric Analysis of Dehydroepiandrosterone and Related Steroids Utilizing a Temperature-Responsive Stationary Phase, Chromatography, 35, 131-138, (2014), DOI: 10.15583/jpchrom.2014.021.
Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Hydrophobized Thermoresponsive Copolymer Brushes for Cell Separation by Multistep Temperature Change, Biomacromolecules, 14(10), 3423-3433, (2013), DOI: 10.1021/bm4006722.
Masuda T, Hidaka M, Murase Y, Akimoto A M, Nagase K, Okano T, Yoshida R, Self-Oscillating Polymer Brushes, Angewandte Chemie International Edition, 52(29), 7468-7471, (2013), DOI: 10.1002/anie.201301988.
Tamura A, Nishi M, Kobayashi J, Nagase K, Yajima H, Yamato M, Okano T, Simultaneous Enhancement of Cell Proliferation and Thermally Induced Harvest Efficiency Based on Temperature-Responsive Cationic Copolymer-Grafted Microcarriers, Biomacromolecules, 13(6), 1765-1773, (2012), DOI: 10.1021/bm300256e.
Pirraco R P, Yamato M, Akiyama Y, Nagase K, Nakayama M, Marques A P, Reis R L, Okano T, Responsive Polymer Coatings for Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet Engineering, Intelligent Surfaces in Biotechnology, pp. 321-354 (2012), DOI 10.1002/9781118181249.ch9.
Nagase K, Mukae N, Kikuchi A, Okano T, Thermally Modulated Retention of Lymphocytes on Polymer-Brush-Grafted Glass Beads, Macromolecular Bioscience, 12(3), 333-340, (2012), DOI: 10.1002/mabi.201100283, IF: 5.859, (Front Cover Article)
Nagase K, Kimura A, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T, Dynamically cell separating thermo-functional biointerfaces with densely packed polymer brushes, Journal of Materials Chemistry, 22(37), 19514-19522, (2012), DOI: 10.1039/C2JM31797D.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, High Stability of Thermoresponsive Polymer-Brush-Grafted Silica Beads as Chromatography Matrices, DOI: 10.1021/am201832b, ACS Applied Materials & Interfaces, 4(4), 1998-2008, (2012).
Nagase K, Yuk S F, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermo-responsive protein adsorbing materials for purifying pharmaceutical protein on exposed charging surface, Journal of Materials Chemistry, 21(8), 2590-2593, (2011), DOI: 10.1039/C0JM03453C.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermally-modulated on/off-adsorption materials for pharmaceutical protein purification, Biomaterials, 32(2), 619-627, (2011), DOI: 10.1016/j.biomaterials.2010.09.012.
Nagase K, Watanabe M, Kikuchi A, Yamato M, Okano T, Thermo-Responsive Polymer Brushes as Intelligent Biointerfaces: Preparation via ATRP and Characterization, Macromolecular Bioscience, 11(3), 400-409, (2011), DOI: 10.1002/mabi.201000312, IF: 5.859, (Front Cover Article).
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Thermoresponsive Polymer Brush on Monolithic-Silica-Rod for the High-Speed Separation of Bioactive Compounds, Langmuir, 27(17), 10830-10839, (2011), DOI: 10.1021/la201360p.
Nagase K, Mizutani Akimoto A, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Effect of reaction solvent on the preparation of thermo-responsive stationary phase through a surface initiated atom transfer radical polymerization, Journal of Chromatography A, 1218(48), 8617-8628, (2011), DOI: 10.1016/j.chroma.2011.09.082.
Nagase K, Kumazaki M, Kanazawa H, Kobayashi J, Kikuchi A, Akiyama Y, Annaka M, Okano T, Thermoresponsive Polymer Brush Surfaces with Hydrophobic Groups for All-Aqueous Chromatography, ACS Applied Materials & Interfaces, 2(4), 1247-1253, (2010), DOI: 10.1021/am100122h.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Annaka M, Okano T, Preparation of Thermoresponsive Anionic Copolymer Brush Surfaces for Separating Basic Biomolecules, Biomacromolecules, 11(1), 215-223, (2010), DOI: 10.1021/bm9010744.
Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads, Journal of Chromatography B, 878(24), 2191-2198, (2010), DOI: 10.1016/j.jchromb.2010.06.026.
Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides, Journal of Chromatography A, 1217(38), 5978-5985, (2010), DOI: 10.1016/j.chroma.2010.07.067.
Mizutani A, Nagase K, Kikuchi A, Kanazawa H, Akiyama Y, Kobayashi J, Annaka M, Okano T, Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography, Journal of Chromatography A, 1217(4), 522-529, (2010), DOI: 10.1016/j.chroma.2009.11.073.
Idota N, Nagase K, Tanaka K, Okano T, Annaka M, Stereoregulation of Thermoresponsive Polymer Brushes by Surface-Initiated Living Radical Polymerization and the Effect of Tacticity on Surface Wettability, Langmuir, 26(23), 17781-17784, (2010), DOI: 10.1021/la1024229.
Hattori Y, Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Hydration of poly(N-isopropylacrylamide) brushes on micro-silica beads measured by a fluorescent probe, Chemical Physics Letters, 491(4-6), 193-198, (2010), DOI: 10.1016/j.cplett.2010.03.082.
Nagase K, Kobayashi J, Okano T, Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering, Journal of The Royal Society Interface, 6(Suppl 3), S293-S309, (2009), DOI: 10.1098/rsif.2008.0499.focus.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Annaka M, Kanazawa H, Okano T, Influence of Graft Interface Polarity on Hydration/Dehydration of Grafted Thermoresponsive Polymer Brushes and Steroid Separation Using All-Aqueous Chromatography, Langmuir, 24(19), 10981-10987, (2008), DOI: 10.1021/la801949w.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Effects of Graft Densities and Chain Lengths on Separation of Bioactive Compounds by Nanolayered Thermoresponsive Polymer Brush Surfaces, Langmuir, 24(2), 511-517, (2008), DOI: 10.1021/la701839s.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Preparation of Thermoresponsive Cationic Copolymer Brush Surfaces and Application of the Surface to Separation of Biomolecules, Biomacromolecules, 9(4), 1340-1347, (2008), DOI: 10.1021/bm701427m.
Nagase K, Kobayashi J, Kikuchi A, Akiyama Y, Kanazawa H, Okano T, Interfacial Property Modulation of Thermoresponsive Polymer Brush Surfaces and Their Interaction with Biomolecules, Langmuir, 23(18), 9409-9415, (2007), DOI: 10.1021/la700956b.
Annaka M, Yahiro C, Nagase K, Kikuchi A, Okano T, Real-time observation of coil-to-globule transition in thermosensitive poly(N-isopropylacrylamide) brushes by quartz crystal microbalance, Polymer, 48(19), 5713-5720, (2007), DOI: 10.1016/j.polymer.2007.06.067.
Nagase K, Kohori F, Sakai K, Nishide H, Rearrangement of hollow fibers for enhancing oxygen transfer in an artificial gill using oxygen carrier solution, Journal of Membrane Science, 254(1-2), 207-217, (2005), DOI: 10.1016/j.memsci.2005.01.008.
Nagase K, Kohori F, Sakai K, Oxygen transfer performance of a membrane oxygenator composed of crossed and parallel hollow fibers, Biochemical Engineering Journal, 24(2), 105-113, (2005), DOI: 10.1016/j.bej.2005.02.003.
Nagase K, Hasegawa U, Kohori F, Sakai K, Nishide H, The photoresponse of a molybdenum porphyrin makes an artificial gill feasible, Journal of Membrane Science, 249(1-2), 235-243, (2005), DOI: 10.1016/j.memsci.2004.10.040.
Nagase K, Kohori F, Sakai K, Efficiency of biological and artificial gills, Bio-mechanisms of Swimming and Flying, 188-199, (2004), DOI: 10.1007/978-4-431-53951-3_14.
Nagase K, Kohori F, Sakai K, Designing an Artificial Gill Using Oxygen Carrier Solution for Effective Supply of Oxygen to Underwater Closed Spaces, Journal of Research in Science and Engineering, 1, 31-36, (2003).
Nagase K, Kohori F, Sakai K, Development of a compact artificial gill using concentrated hemoglobin solution as the oxygen carrier, Journal of Membrane Science, 215(1-2), 281-292, (2003), DOI: 10.1016/s0376-7388(03)00021-8.
Nagase K, Matsuda N, Sakai K, Optimum control of oxygen affinity of hemoglobin as an oxygen carrier solution for an artificial gill, Journal of Artificial Organs, 5(1), 44-49, (2002), DOI: 10.1007/s100470200007.

Publications (Kinoshita-Kikuta Emiko)

Highlighted Publications (Kinoshita-Kikuta Emiko)

Kikuta, E., Murata, M., Katsube, N., Koike, T., Kimura, E. Novel recognition of thymine base in double-stranded DNA by zinc(II)−macrocyclic tetraamine complexes appended with aromatic groups. Journal of the American Chemical Society, 121: 5426–5436, (1999)
Kikuta, E., Aoki, S., Kimura, E. A new type of potent inhibitors of HIV-1 TAR RNA-Tat peptide binding by zinc(II)−macrocyclic tetraamine complexes. Journal of the American Chemical Society, 123: 7911–7912, (2001)
Kinoshita-Kikuta E., Kinoshita E., Koike T. A novel procedure for simple and efficient genotyping of single nucleotide polymorphisms by using the Zn2+–cyclen complex. Nucleic Acids Research, 30: e126, (2002)
Kimura, E., Aoki, A., Kikuta, E., Koike T. A macrocyclic zinc(II) fluorophore as a detector of apoptosis. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 100: 3731–3736, (2003)
Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., Koike, T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Molecular & Cellular Proteomics, 5: 749–757, (2006)
Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E., Koike, T. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis. Molecular & Cellular Proteomics, 6: 356–366, (2007)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE. Nature Protocols, 9: 1513–1521, (2009)
Kinoshita-Kikuta E., Utsumi T., Miyazaki A., Tokumoto C., Doi K., Harada H., Kinoshita E., Koike T. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Scientific Reports, 10: 16273, (2020)

Publications (Kinoshita-Kikuta Emiko)

Ichimaru, Y., Kato, K., Nakatani, R., Augiura, K., Mizutani, H., Kinoshita-Kikuta E., Koike, T., Jin, W., Imai, M., Kurosaki, H. Characterization of zinc(II) complex of 1,4,7,10-tetraazacyclodecane and deprotonated 5-fluorouracil (FU) in crystalline/solution states and evaluation of anticancer activity: approach for improving the anticancer activity of FU. Inorg. Chem. Comm., 147:110221 (2023)
Kinoshita-Kikuta, E., Ino, Y., Kimura, Y., Akiyama, T., Kinoshita, E., Koike, T., Use of Escherichia coli expression system for analyzing kinase motifs. J. Electrophoresis, 66:13-20 (2022)
Ino Y., Kinoshita E., Kinoshita-Kikuta E., Akiyama T., Nakai Y., Ryo A., Hirano H., Koike T., Kimura Y. Evaluation of four phosphopeptide enrichment strategies for mass spectrometry–based proteomic analysis. Proteomics, 22, e2100216 (2022)
Ichimaru, Y., Kato, K., Jin W., Sugiura K., Kinoshita-Kikuta E., Kinoshita E., Kurosaki H., Koike T. Crystal structure of bis{1,3-bis[bis(pyridine-2-ylmethyl)amino]propan-2-olato-dizinc(II)}-orthophosphate tirs(percholate) octahydrate, [Phos-tag]2-PO43-)](ClO4-)3·8H2O. X-ray Struct. Anal., 37: 87-88 (2021)
Kinoshita-Kikuta E., Ichimaru Y., Yamano Y., Kato K., Kurosaki H., Kinoshita E., Koike T. Characterization of the Binding of Adenosine-5’-monophosphate to a µ-Type Alkoxide-linked Dinuclear Zinc(II) Complex in Crystal and Solution State. Bull. Chem. Soc. Jap., 94:2670-2677 (2021)
Kinoshita-Kikuta E., Kinoshita E., Suga M., Higashida M., Yamane Y., Nakamura T., Koike T. Characterization of phosphorylation status and kinase activity of Src family kinases expressed in cell-based and cell-free protein expression systems. Biomolecules, 11: 1448, (2021)
Kinoshita-Kikuta E., Yoshimoto M., Yano M., Kinoshita E., Koike T. An assay of human tyrosine protein kinase activities by using an Escherichia coli protein-expression system. BioTechniques, 70: 209-217 (2021)
Sunehiro M., Sasaki K., Kinoshita-Kikuta E., Kinoshita E., Koike T. Phos-tag-based micropipette-tip method for analysis of phosphomonoester-type impurities in synthetic oligonucleotides. J. Chromatogr. B, 1151: 122198 (2020)
Kinoshita-Kikuta E., Akayama K., Kinoshita E., Koike T. A dot-blot-staining method for detecting phosphoproteins with a Phos-tag Aqua fluorescent dye. J Electrophoresis, 64: 7-11 (2020)
Kinoshita-Kikuta E., Utsumi T., Miyazaki A., Tokumoto C., Doi K., Harada H., Kinoshita E., Koike T. Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep., 10: 16273, (2020)
Kinoshita-Kikuta E., Maruta S., Eguchi Y., Igarashi M., Okajima T., Utsumi R., Kinoshita E., Koike T. An immuno-dot blot assay for screening histidine kinase inhibitors. Anal. Biochem., 600: 113765 (2020)
Tsunehiro M, Sasaki K, Kinoshita-Kikuta E, Kinoshita E, Koike T. Phos-tag-based micropipette-tip method for analysis of phosphomonoester-type impurities in synthetic oligonucleotides. J. Chromatogr. B, 1151:122198 (2020)
Kinoshita-Kikuta E., Tanikawa A., Hosokawa T., Kiwado A., Moriya K., Kinoshita E., Koike T., Utsumi T. A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phos-tag SDS-PAGE. PLoS One, 14: e0225510 (2019)
Kinoshita-Kikuta E., Kusamoto H., Ono S., Akayama K., Eguchi Y., Igarashi M., Okajima T., Utsumi R., Kinoshita E, Koike T. Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes. Electrophoresis, 40: 3005–3013 (2019)
Kusamoto H., Kinoshita-Kikuta E., Nishimura T., Nagai T., Kinoshita E., Koike T. Gel-based analysis of protein phosphorylation status by rapid fluorometric staining using TAMRA-labeled Phos-tag. J. Electrophoresis, 63: 25-32 (2019)
Kinoshita-Kikuta E., Kinoshita E., Ueda S., Ino Y., Kimura Y., Hirano H., Koike T. Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers. Biochim. Biophys. Acta (BBA) - Proteins and Proteomics 2018, 1867: 62-70 (2019)
Uezato Y., Kameshita I., Morisawa K., Sakamoto S., Kinoshita E., Kinoshita-Kikuta E., Koike T., Sugiyama Y. A method for profiling the phosphorylation state of tyrosine protein kinases. Biochim. Biophys. Acta (BBA) - Proteins and Proteomics 2018, 1867: 71-75 (2019)
Kusamoto H., Shiba A., Tsunehiro M., Fujioka H., Kinoshita-Kikuta E., Kinoshita E., Koike T. A simple method for determining the ligand affinity toward a zinc-enzyme. Dalton Trans., 47: 1841-1848 (2018)
Yuan E. T., Ino Y., Kawaguchi M., Kimura Y., Hirano H., Kinoshita-Kikuta E., Kinoshita E., Koike T. A Phos-tag-based micropipette-tip method for rapid and selective enrichment of phosphopeptides Electrophoresis, 38: 2447–2455 (2017)
Shiba A., Kinoshita-Kikuta E., Kinoshita E., Koike T. TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase. Sensors, 17: 1877 (2017)
Kinoshita, E., Kinoshita-Kikuta, E., Karata, K., Kawano, T., Nishiyama, A., Yamato, M., Koike, T. Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos-tag SDS-PAGE migration. Electrophoresis, 38: 1139-1146 (2017)
Kusamoto, H., Shiba, A., Koretake, N., Fujioka, H., Hieda, Y., Kinoshita-Kikuta, E., Kinoshita, E., koike, T. A novel thiol-affinity micropipette tip method using zinc(II)–cyclen-attached agarose beads for enrichment of cysteine-containing molecules. J. Chromatogr. B, 1031: 195-201 (2016)
Kinoshita E, Kinoshita-Kikuta E, Kubota Y, Takekawa M, Koike T. A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1. Proteomics, 16:1825-36 (2016)
Kinoshita-Kikuta, E., Eiji Kinoshita, Eguchi, Y., Koike, T. Validation of cis and trans modes in multistep phosphotransfer signaling of bacterial tripartite sensor kinases by using Phos-tag SDS-PAGE. PLoS One, 11: e0148294 (2016)
Kinoshita-Kikuta, E., Eiji Kinoshita, Eguchi, Y., Yanagihara, S., Edahiro, K., Inoue, Y., Taniguchi, M, Yoshida, M., Yamamoto, K., Takahashi, H., Sawasaki , T., Utsumi, R., Koike, T. Functional characterization of the receiver domain for phosphorelay control in hybrid sensor kinases. PLoS One, 10: e0132598 (2015)
Sugiyama, Y., Katayama, S., Kameshita, I., Morisawa, K., Higuchi, T., Todaka, H., Eiji Kinoshita, Kinoshita-Kikuta, E., Koike, T., Taniguchi, T., Sakamoto, S. Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE. MethodsX, 2: 469–474 (2015)
Kinoshita-Kikuta, E., Kinoshita, E., Matsuda, A., Koike, T. Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel. Proteomics, 14: 2437-2442, (2014)
Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. Identification of two phosphorylated species of ß-catenin involved in the ubiquitin-proteasome pathway by using two-dimensional Phos-tag affinity electrophoresis. J. Electrophoresis, 58: 1-4 (2014)
Kinoshita-Kikuta, E., Kurosaki, H., Kunisada, N., Kinoshita, E., Koike, T. A Phos-tag-based fluorescence quenching system for activity assay and inhibitor screening for alkaline phosphatase. Am. J. Anal. Chem., 5: 796-804, (2014)
Fujioka, H., Tsunehiro, M., Kawaguchi, M., Kuramoto, Y., Kurosaki, H., Hieda, Y., Kinoshita-Kikuta, E., Kinoshita, E., Koike,T. Simple enrichment of thiol-containing biomolecules by using zinc(II)–cyclen-functionalized magnetic beads, J. Sep. Sci., 37, 1601-1609 (2014)
Kinoshita, E., Kinoshita-Kikuta, E., Shiba, A., Edahiro, K., Inoue, Y., Yamamoto, K., Yoshida, M., Koike, T. Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: evaluation of adenosine 5′-O-(3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions. Proteomics, 14: 668-679 (2014)
Fujioka H., Hieda Y., Kuramonot Y., konishi K., Kinoshita-Kikuta E., Kinoshita E., Koike T. Increase in lipophilicity of enalaprilat by complexation with copper (II) or zinc (II) ions. Yakugaku Zasshi, 133: 1135-1141 (2013)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag. Anal. Biochem., 438: 104-106, (2013)
Tsunehiro, M., Meki, Y., Matsuoka, K., Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules. J. Chromatogr. B, 925: 86–94 (2013)
Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. A laborsaving, timesaving, and more reliable strategy for separation of low-molecular-mass phosphoproteins in Phos-tag affinity electrophoresis. Int. J. Chem., 4: e1-8 (2012)
Kinoshita, E., Kinoshita-Kikuta, E., Sugiyama, Y., Fukuda, Y., Ozeki, T., Koike, T. Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin. Proteomics, 12: 932-937 (2012)
Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis. Electrophoresis, 33: 849-855 (2012)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions, Proteomics, 12:192-202 (2012)
Kinoshita-Kikuta, E., Yamada, A., Inoue, C., Kinoshita, E., Koike, T. A novel phosphate-affinity bead with immobilized Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins. J. Integ. Omics, 1: 157-169 (2011)
Kinoshita, E., Kinoshita-Kikuta, E., Harada, N., Koike, T. Zinc(II)–cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments. Anal. Biochem., 408: 348-350 (2011)
Somura, M., Takiyama, K., Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. A phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide. Anal. Methods, 3: 1303-1309 (2011)
Kinoshita, E., Kinoshita-Kikuta, E. Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling. Proteomics 11: 319-323 (2011)
Sugiyama, Y., Hatano, N., Sueyoshi, N., Suetake, I., Tajima, S., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Kameshita, I. The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1d/e. Biochem. J., 427: 489-497 (2010)
Kinoshita, E., Kinoshita-Kikuta, E., Nakashima, H., Koike, T. Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes. Clin. Biochem., 43: 302-306 (2010)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE. Nat. Protoc., 9: 1513–1521, (2009)
Kinoshita, E., Kinoshita-Kikuta, E., Ujihara, H., Koike, T. Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose. Proteomics, 9: 4098–4101 (2009)
Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates. Anal. Biochem., 389: 83–85 (2009)
Takiyama, K., Kinoshita, E., Kinoshita-Kikuta, E., Fujioka, Y., Kubo, Y., Koike, T. A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides. Anal. Biochem., 388: 235–241 (2009)
Kinoshita, E., Kinoshita-Kikuta, E., Matsubara, M., Aoki, Y., Ohie, S., Mouri, Y., Koike, T. Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes. Electrophoresis, 30: 550–559 (2009)
Ishiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Tashiro, S., Elledge, S.J., Takata, M. FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. Nat. Struct.Mol. Biol., 15: 1138–1146 (2008)
Kinoshita, E., Kinoshita-Kikuta, E., Yoshimoto, M., Koike, T. Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn2+–cyclen polyacrylamide gel electrophoresis. Anal. Biochem., 380: 122–127 (2008)
Kinoshita, E., Kinoshita-Kikuta, E., Matsubara, M., Yamada, S., Nakamura, H., Shiro, Y., Aoki, Y., Okita, K., Koike, T. Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. Proteomics, 8: 2994–3003 (2008)
Kinoshita-Kikuta, E., Kinoshita, E., Koike, T. A mobility shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamidegel electrophoresis. Anal. Biochem., 378: 102–104 (2008)
Nakanishi, T., Ando, E., Furuta, M., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Tsunasawa, S., Nishimura, O. Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate binding tag molecule. J. Biomol. Tech., 18: 278–286 (2007)
Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E., Koike, T. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis. Mol. Cell. Proteomics, 6: 356–366, (2007)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis. Anal. Biochem., 361: 294–298 (2007)
Nakano, Y., Tashiro, S. Kinoshita, E., Kinoshita-Kikuta, E., Takenaka, S., Miyoshi, M., Ogi, H., Sakoda, E., Oda, N., Suenari, K., Tonouchi, Y., Okimoto, T., Hirai, Y., Miura, F., Yamaoka, K., Koike, T., Chayama, K. Non-SCN5A related Brugada syndromes: Verification of normal splicing and trafficking of SCN5A without exonic mutations. Ann. Hum. Genet., 71: 8–17, (2007)
Yamada, S., Nakamura, H., Kinoshita, E., Kinoshita-Kikuta, E., Koike, T., Shiro, Y. Separation of a phosphorylated-His protein using phosphate-affinity polyacrylamide gel electrophoresis. Anal. Biochem., 360: 160–162 (2007)
Kinoshita-Kikuta, E., Kinoshita, E., Yamada, A., Endo, M., Koike T. Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH. Proteomics, 6: 5088–5095 (2006)
Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K., Koike, T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol. Cell. Proteomics, 5: 749–757 (2006)
Kinoshita, E., Kinoshita-Kikuta, E., Kojima, H., Nakano, Y., Chayama, K., Koike, T. Reliable and cost-effective screening of inherited heterozygosity by using Zn2+–cyclen polyacrylamide gel electrophoresis. Clin. Chem., 51: 2195–2198 (2005)
Kinoshita, E., Ishikawa, K., Kinoshita-Kikuta, E., Ohtani, K., Shiro, M., Koike, T. An alkoxide-bridged dinuclear zinc(II) hexaazacryptate: a novel phosphate capture molecule in aqueous solution. Bull. Chemi. Soc. Jap., 78:125–131 (2005)
Kinoshita, E., Yamada, A., Takeda, H., Kinoshita-Kikuta, E., Koike T. Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins. J. Sep. Sci., 28: 155–162 (2005)
Kinoshita, E., Kinoshita-Kikuta, E., Koike, T. A heteroduplex-preferential Tm depressor for the specificity-enhanced DNA polymerase chain reactions. Anal. Biochem., 337: 154–160, (2005)
Kimura, E., Aoki, A., Kikuta, E., Koike T. A macrocyclic zinc(II) fluorophore as a detector of apoptosis. Proc. Nat. Acad. Sci. USA, 100: 3731–3736 (2003)
Kinoshita-Kikuta E, Kinoshita E, Koike T. A novel procedure for simple and efficient genotyping of single nucleotide polymorphisms by using the Zn2+–cyclen complex. Nuc. Acids Res., 30: e126, (2002)
Kikuta, E., Aoki, S., Kimura, E. New potent agents binding to a poly(dT) sequence in double-stranded DNA: bis(Zn2+−cyclen) and tris(Zn2+−cyclen) complexes. J. Biol. Inorg. Chem., 7: 473–482 (2002)
Kikuta, E., Aoki, S., Kimura, E. A new type of potent inhibitors of HIV-1 TAR RNA-Tat peptide binding by zinc(II)−macrocyclic tetraamine complexes. J. Am. Chem. Soc., 123: 7911–7912 (2001)
Kikuta, E., Matsubara, R., Katsube, N., Koike, T., Kimura, E. Selective recognition of consecutive G sequence in double-stranded DNA by zinc(II)-macrocyclic tetraamine complex appended with an anthraquinone. J. Inorg. Biochem., 82: 239–249 (2000)
Kikuta E., Koike, T., Kimura E. Controlling gene expression by zinc(II)-macrocyclic tetraamine complexes. J. Inorg. Biochem., 79: 253–259 (2000)
Kikuta, E., Katsube, N., Kimura, E. Natural and synthetic double-stranded DNA binding studies of macrocyclic tetraamine complexes. J. Biol. Inorg. Chem., 4: 431–440 (1999)
Kikuta, E., Murata, M., Katsube, N., Koike, T., Kimura, E. Novel recognition of thymine base in double-stranded DNA by zinc(II)−macrocyclic tetraamine complexes appended with aromatic groups. J. Am. Chem. Soc., 121: 5426–5436 (1999)