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An experiment is a procedure carried out to support or refute a hypothesis, or determine the efficacy or likelihood of something previously untried. Experiments provide insight into cause-and-effect by demonstrating what outcome occurs when a particular factor is manipulated. Experiments vary greatly in goal and scale but always rely on repeatable procedure and logical analysis of the results. There also exist natural experimental studies.

A child may carry out basic experiments to understand how things fall to the ground, while teams of scientists may take years of systematic investigation to advance their understanding of a phenomenon. Experiments and other types of hands-on activities are very important to student learning in the science classroom. Experiments can raise test scores and help a student become more engaged and interested in the material they are learning, especially when used over time.[1] Experiments can vary from personal and informal natural comparisons (e.g. tasting a range of chocolates to find a favorite), to highly controlled (e.g. tests requiring complex apparatus overseen by many scientists that hope to discover information about subatomic particles). Uses of experiments vary considerably between the natural and human sciences.

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Experiments typically include controls, which are designed to minimize the effects of variables other than the single independent variable. This increases the reliability of the results, often through a comparison between control measurements and the other measurements. Scientific controls are a part of the scientific method. Ideally, all variables in an experiment are controlled (accounted for by the control measurements) and none are uncontrolled. In such an experiment, if all controls work as expected, it is possible to conclude that the experiment works as intended, and that results are due to the effect of the tested variables.

In the scientific method, an experiment is an empirical procedure that arbitrates competing models or hypotheses.[2][3] Researchers also use experimentation to test existing theories or new hypotheses to support or disprove them.[3][4]

An experiment usually tests a hypothesis, which is an expectation about how a particular process or phenomenon works. However, an experiment may also aim to answer a "what-if" question, without a specific expectation about what the experiment reveals, or to confirm prior results. If an experiment is carefully conducted, the results usually either support or disprove the hypothesis. According to some philosophies of science, an experiment can never "prove" a hypothesis, it can only add support. On the other hand, an experiment that provides a counterexample can disprove a theory or hypothesis, but a theory can always be salvaged by appropriate ad hoc modifications at the expense of simplicity.

In engineering and the physical sciences, experiments are a primary component of the scientific method. They are used to test theories and hypotheses about how physical processes work under particular conditions (e.g., whether a particular engineering process can produce a desired chemical compound). Typically, experiments in these fields focus on replication of identical procedures in hopes of producing identical results in each replication. Random assignment is uncommon.

In medicine and the social sciences, the prevalence of experimental research varies widely across disciplines. When used, however, experiments typically follow the form of the clinical trial, where experimental units (usually individual human beings) are randomly assigned to a treatment or control condition where one or more outcomes are assessed.[5] In contrast to norms in the physical sciences, the focus is typically on the average treatment effect (the difference in outcomes between the treatment and control groups) or another test statistic produced by the experiment.[6] A single study typically does not involve replications of the experiment, but separate studies may be aggregated through systematic review and meta-analysis.

There are various differences in experimental practice in each of the branches of science. For example, agricultural research frequently uses randomized experiments (e.g., to test the comparative effectiveness of different fertilizers), while experimental economics often involves experimental tests of theorized human behaviors without relying on random assignment of individuals to treatment and control conditions.

In some disciplines (e.g., psychology or political science), a 'true experiment' is a method of social research in which there are two kinds of variables. The independent variable is manipulated by the experimenter, and the dependent variable is measured. The signifying characteristic of a true experiment is that it randomly allocates the subjects to neutralize experimenter bias, and ensures, over a large number of iterations of the experiment, that it controls for all confounding factors.[14]

Depending on the discipline, experiments can be conducted to accomplish different but not mutually exclusive goals: [15] test theories, search for and document phenomena, develop theories, or advise policymakers. These goals also relate differently to validity concerns.

A controlled experiment often compares the results obtained from experimental samples against control samples, which are practically identical to the experimental sample except for the one aspect whose effect is being tested (the independent variable). A good example would be a drug trial. The sample or group receiving the drug would be the experimental group (treatment group); and the one receiving the placebo or regular treatment would be the control one. In many laboratory experiments it is good practice to have several replicate samples for the test being performed and have both a positive control and a negative control. The results from replicate samples can often be averaged, or if one of the replicates is obviously inconsistent with the results from the other samples, it can be discarded as being the result of an experimental error (some step of the test procedure may have been mistakenly omitted for that sample). Most often, tests are done in duplicate or triplicate. A positive control is a procedure similar to the actual experimental test but is known from previous experience to give a positive result. A negative control is known to give a negative result. The positive control confirms that the basic conditions of the experiment were able to produce a positive result, even if none of the actual experimental samples produce a positive result. The negative control demonstrates the base-line result obtained when a test does not produce a measurable positive result. Most often the value of the negative control is treated as a "background" value to subtract from the test sample results. Sometimes the positive control takes the quadrant of a standard curve.

An example that is often used in teaching laboratories is a controlled protein assay. Students might be given a fluid sample containing an unknown (to the student) amount of protein. It is their job to correctly perform a controlled experiment in which they determine the concentration of protein in the fluid sample (usually called the "unknown sample"). The teaching lab would be equipped with a protein standard solution with a known protein concentration. Students could make several positive control samples containing various dilutions of the protein standard. Negative control samples would contain all of the reagents for the protein assay but no protein. In this example, all samples are performed in duplicate. The assay is a colorimetric assay in which a spectrophotometer can measure the amount of protein in samples by detecting a colored complex formed by the interaction of protein molecules and molecules of an added dye. In the illustration, the results for the diluted test samples can be compared to the results of the standard curve (the blue line in the illustration) to estimate the amount of protein in the unknown sample.

Controlled experiments can be performed when it is difficult to exactly control all the conditions in an experiment. In this case, the experiment begins by creating two or more sample groups that are probabilistically equivalent, which means that measurements of traits should be similar among the groups and that the groups should respond in the same manner if given the same treatment. This equivalency is determined by statistical methods that take into account the amount of variation between individuals and the number of individuals in each group. In fields such as microbiology and chemistry, where there is very little variation between individuals and the group size is easily in the millions, these statistical methods are often bypassed and simply splitting a solution into equal parts is assumed to produce identical sample groups.

Once equivalent groups have been formed, the experimenter tries to treat them identically except for the one variable that he or she wishes to isolate. Human experimentation requires special safeguards against outside variables such as the placebo effect. Such experiments are generally double blind, meaning that neither the volunteer nor the researcher knows which individuals are in the control group or the experimental group until after all of the data have been collected. This ensures that any effects on the volunteer are due to the treatment itself and are not a response to the knowledge that he is being treated.

The term "experiment" usually implies a controlled experiment, but sometimes controlled experiments are prohibitively difficult, impossible, unethical or illegal. In this case researchers resort to natural experiments or quasi-experiments.[16] Natural experiments rely solely on observations of the variables of the system under study, rather than manipulation of just one or a few variables as occurs in controlled experiments. To the degree possible, they attempt to collect data for the system in such a way that contribution from all variables can be determined, and where the effects of variation in certain variables remain approximately constant so that the effects of other variables can be discerned. The degree to which this is possible depends on the observed correlation between explanatory variables in the observed data. When these variables are not well correlated, natural experiments can approach the power of controlled experiments. Usually, however, there is some correlation between these variables, which reduces the reliability of natural experiments relative to what could be concluded if a controlled experiment were performed. Also, because natural experiments usually take place in uncontrolled environments, variables from undetected sources are neither measured nor held constant, and these may produce illusory correlations in variables under study. 2351a5e196