Research experience

Protein expression: SARS-CoV-1/2 S, virus-like particle (VP1), nanobodies, ACE2, IgM, enzymes, disordered proteins and pseudoviruses.

Biomolecule purification: Affinity chromatography (His, GST and MBP), ion-exchange chromatography, and gel filtration.

Biomolecule modification: Sortase A transpeptidation, click chemistry, BirA biotinylation, and chemical conjugations.

Biomolecule analysis: Binding kinetics, dynamic light scattering, and electrospray ionization mass spectrometry analysis.

Microscopy: STED (Abberior), Confocal (Airyscan LSM 980; Nikon A1, C1), Expansion microscopy and TEM (FEI T20).

Cell culture: HeLa cells, HEK 293 variants, Expi 293F, A549, Pichia pastoris, Pseudomonas putida and Escherichia coli.

Cell analysis: Flow cytometry, stable cell line sorting, cell fixing and immunostaining, and live-cell imaging.

Software proficiency: PyMol ^®, Fiji ^®_, FlowJo ^® Statistica^®, Design Expert ^®_, BioRender ^®, and Microsoft Office.