Publications

Journal Articles

2025

Removal and monitoring of residual nucleic acids from core streptavidin inclusion bodies for increased refolding yield. Alias, Nurul Nadia Mohamad, Eugene Boon Beng Ong, and Mervyn WO Liew. Protein Expression and Purification 225 (2025): 106591. https://doi.org/10.1016/j.pep.2024.106591

2024

TolC facilitates the intracellular survival and immunomodulation of Salmonella Typhi in human host cells. Hussain, Ashraf, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, and Phua Kia Kien. Virulence 15, no. 1 (2024): 2395831. https://doi.org/10.1080/21505594.2024.2395831

Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi. Ahmad Najib, Mohamad, Anja Winter, Khairul Mohd Fadzli Mustaffa, Eugene Boon Beng Ong, Kasturi Selvam, Muhammad Fazli Khalid, Mohd Syafiq Awang, Asrulnizam Abd Manaf, Yazmin Bustami, and Ismail Aziah. Scientific Reports 14, no. 1 (2024): 28416.

Academic Workloads: Insights from Medical and Non-medical Lecturers' Experience in Universiti Sains Malaysia. Bahri Yusoff, Muhamad Saiful, Abdul Latiff, Abdul Rais, Eugene Boon Beng Ong, Nor Asmat Ismail, Abdul Rahim, Nur Arzuar et al. Education in Medicine Journal 16, no. 3 (2024). https://doi.org/10.21315/eimj2024.16.3.10

Production of recombinant human epidermal growth factor in Escherichia coli: Strategic upstream and downstream considerations for high protein yield. Wong, Ru Shen, Mervyn WO Liew, and Eugene Boon Beng Ong. Process Biochemistry (2024). https://doi.org/10.1016/j.procbio.2024.07.022

Unravelling bacterial virulence factors in yeast: From identification to the elucidation of their mechanisms of action. Wong, ZhenPei, and Eugene Boon Beng Ong. Archives of Microbiology 206, no. 7 (2024): 303. https://doi.org/10.1007/s00203-024-04023-2

Exploring the role of impedance spectroscopy in assessing 405 nm laser-induced inactivation of saccharomyces cerevisiae. Ang, Beng Jiong, Nursakinah Suardi, Eugene Boon Beng Ong, Siti Nur Hazieqah Khasim, Sylvester Jande Gemanam, Iskandar Shahrim Mustafa, and Jing Heng Fong. Photochemical & Photobiological Sciences 23, no. 5 (2024): 931-940. https://doi.org/10.1007/s43630-024-00564-z

2023

Deletion of Salmonella enterica serovar Typhi tolC reduces bacterial adhesion and invasion toward host cells. A Hussain, EBB Ong, P Balaram, A Ismail, PK Kien Frontiers in Microbiology 14, 1301478. https://doi.org/10.3389/fmicb.2023.1301478

Intensification of Inclusion Body Processing via Temperature-Based Refolding. Ru Shen Wong, Nurul Nadia Mohamad Alias, Eugene Boon Beng Ong & Mervyn W. O. Liew. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY. doi.org/10.1007/978-1-0716-2930-7_13

2022

Identifying Leptospira interrogans putative virulence factors with a yeast protein expression screen. Weng Yu Lai, Zhenpei Wong, Chiat Han Chang, Mohd Razip Samian, Nobumoto Watanabe, Aik-Hong Teh, Rahmah Noordin, Eugene Boon Beng Ong. Appl Microbiol Biotech, 2022, 1-15. doi.org/10.1007/s00253-022-12160-1

2021

Identification of Tropical Plant Extracts That Extend Yeast Chronological Life Span. Kwong, Mandy Mun Yee; Lee, Jee Whu; Samian, Mohammed Razip; A Wahab, Habibah; Watanabe, Nobumoto; Ong, Eugene Boon Beng. Cells 2021, 10(10), 2718; doi.org/10.3390/cells10102718

Genomic Instability and Cellular Senescence: Lessons From the Budding Yeast. Lee, Jee Whu, and Ong, Eugene Boon Beng. Front. Cell Dev. Biol. 8 (2020): 1793. doi.org/10.3389/fcell.2020.619126

2020

Evaluation of Salmonella Typhi antigen YncE alongside HlyE for the detection of typhoid fever and its carriers. Franklin, Freddy; Chong, Chun Wie; Chua, Leong Huat; Anthony, Amy Amilda; Liew, Mervyn WO; Aziah, Ismail; Ong, Eugene Boon Beng. Med Microbiol Immunol. 2020 209, 593–601(2020) . doi: 10.1007/s00430-020-00667-1

2019

Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays. Kwong, Mandy Mun Yee; Lee, Jee Whu; Samian, Mohammed Razip; Watanabe, Nobumoto; Osada, Hiroyuki; Ong, Eugene Boon Beng. J Microbiol Methods. 2019 Dec;167:105743. doi: 10.1016/j.mimet.2019.105743

2018

Malaysia’s future higher education scenarios: fourth industrial revolution and beyond. Muhamad Saiful Bahri Yusoff, AJA Hamid, Eugene Ong Boon Beng, Mohd Ghows Mohd Azzam, Morshidi Sirat. Technical report. Putrajaya: Ministry of Education

2016

Detection of Salivary IgA Antibodies Against the HlyE Antigen as a Diagnosis of Typhoid Fever. Chin, Kai Ling; Redhuan, Nur Eliyana Mohd; Balaram, Prabha; Phua, Kia Kien; Ong, Eugene Boon Beng. J Clin Diagn Res. 2016 Jun;10(6):DM01-3. doi: 10.7860/JCDR/2016/17801.7909.

Creation of chimeric human/rabbit APOBEC1 with HIV-1 restriction and DNA mutation activities. Ikeda, Terumasa; Ong, Eugene Boon Beng; Watanabe, Nobumoto; Sakaguchi, Nobuo; Maeda, Kazuhiko; Koito, Atsushi. Sci Rep. 2016 Jan 7;6:19035. doi: 10.1038/srep19035.

Application of streptavidin mass spectrometric immunoassay tips for immunoaffinity based antibody phage display panning. Chin, Chai Fung; Ler, Lian Wee; Choong, Yee Siew; Ong, Eugene Boon Beng; Ismail, Asma; Tye, Gee Jun; Lim, Theam Soon. J Microbiol Methods. 2016 Jan;120:6-14. doi: 10.1016/j.mimet.2015.11.007.

2015

Multi-isotype antibody responses against the multimeric Salmonella Typhi recombinant hemolysin E antigen. Ong, Eugene Boon Beng; Ignatius, Joshua; Anthony, Amy Amilda; Aziah, Ismail; Ismail, Asma; Lim, Theam Soon. Microbiol Immunol. 2015 Jan;59(1):43-7. doi: 10.1111/1348-0421.12211.

2014

In Silico Investigation of a HIV-1 Vpr Inhibitor Binding Site: Potential for Virtual Screening and anti-HIV Drug Design. Choi, Sy Bing; Choong, Yee Siew; Saito, Akiko; Wahab, Habibah A; Najimudin, Nazalan; Watanabe, Nobumoto; Osada, Hiroyuki; Ong, Eugene Boon Beng. Mol Inform. 2014 Dec;33(11-12):742-8. doi: 10.1002/minf.201400080.

Overexpression, purification and validation of antigenic Salmonella enterica serovar Typhi proteins identified from LC-MS/MS. Chin, Chai Fung; Teh, Boon Aun; Anthony, Amy Amilda; Aziah, Ismail; Ismail, Asma; Ong, Eugene Boon Beng; Lim, Theam Soon. Appl Biochem Biotechnol. 2014 Nov;174(5):1897-906. doi: 10.1007/s12010-014-1173-y.

2013

Cloning, expression, and purification of the hemolysin/cytolysin (HlyE antigen) from Salmonella enterica serovar Typhi: potential application for immunoassay development. Ong, Eugene Boon Beng; Anthony, Amy Amilda; Ismail, Aziah; Ismail, Asma; Lim, Theam Soon. Diagn Microbiol Infect Dis. 2013 Sep;77(1):87-9. doi: 10.1016/j.diagmicrobio.2013.05.010.

Salmonella enterica serovar Typhi cutF is upregulated during environmental stress. Lau, Kwok-Leong; Ong, Eugene Boon Beng; Zainudin, Zainul Fadziruddin; Samian, Mohd Razip; Ismail, Asma; Najimudin, Nazalan.  J Gen Appl Microbiol. 2013;59(3):239-44. doi: 10.2323/jgam.59.153.

2012

Construction and characterization of a Burkholderia pseudomallei wzm deletion mutant. Yuen Chee Wah; Ong, Eugene Boon Beng; Mohamad S, Manaf UA, Najimudin, Nazalan. J Microbiol Biotechnol. 2012 Oct;22(10):1336-42. doi: 10.4014/jmb.1203.03059.

2011

Vipirinin, a coumarin-based HIV-1 Vpr inhibitor, interacts with a hydrophobic region of VPR. Ong, Eugene Boon Beng; Watanabe, Nobumoto; Saito, Akiko; Futamura, Yushi; Abd El Galil, Khaled Hussein; Koito, Atsushi; Najimudin, Nazalan; Osada, Hiroyuki. J Biol Chem. 2011 Apr 22;286(16):14049-56. doi: 10.1074/jbc.M110.185397.

Purification of the Bacillus subtilis regulatory protein AlsR and the localization of its binding site. Ong, Eugene Boon Beng; Muhammad, Tengku Sifzizul Tengku; Samian, Mohd Razip; Najimudin, Nazalan. Asia Pac J Mol Biol Biotechnol 2011 Jan Vol. 1, 29-37

Books and Chapters

1. Improving in vitro diagnostic design with in silico support: Structural bioinformatics to the fore in Sustainable Diagnostics for Low Resource Areas. Choong, Yee Siew; Lim, Theam Soon; Tye, Gee Jun; Ong, Eugene Boon Beng. 2017 (PDF)

2. Improving in vitro diagnostic design with in silico support: Part 1 Sequence-based informatics with simple tools in Sustainable Diagnostics for Low Resource Areas. Ong, Eugene Boon Beng; Lim, Theam Soon; Tye, Gee Jun; Choong, Yee Siew. 2017 (PDF)

3. Malaysia's Future Higher Education Scenarios - Fourth Industrial Revolution and Beyond. Muhamad Saiful Bahri Yusoff, Anees Janee Ali @ Hamid, Eugene Ong Boon Beng, Mohd Ghows Azzam and Morhidi Sirat. (PDF)

4. Intensification of Inclusion Body Processing via Temperature-Based Refolding. Ru Shen Wong, Nurul Nadia Mohamad Alias, Eugene Boon Beng Ong & Mervyn W. O. Liew. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY. doi.org/10.1007/978-1-0716-2930-7_13