Scientific Expertise

Scientific Expertise

Animal Expertise (Handling and Surgery)

  • Efficient in animal handling (especially Wistar rats)

Animal tissue culture techniques

  • Proficiency in Mammalian Tissue culture for various types of normal (L6 myotube and 3T3-L1 adipocyte) and cancerous cell lines (HEK293, Hep G2 and HeLa) cell lines

Oxidative Stress Study

  • Proficiency in biochemical estimation of enzymatic (SGOT/SGPT, ALP, GGT, Catalase, SOD, GST, and GSH) and antioxidant assays

Molecular Biology Techniques

  • Isolation of genomic DNA and RNA, PCR, Purification of plasmid DNA by CsCl density gradient method, gel electrophoresis and RT-PCR

Microbiological Techniques

  • Experience on bacterial culture, pure culture isolation, and antibiotic susceptibility test

Biochemical Techniques

  • Quantitative and qualitative analysis of biomolecules and enzyme assay

Analytical Techniques

  • New enzymatic method developed for the glucose uptake in the cell lines

  • Spectroscopic techniques for analyses - UV-Vis, fluorescence, FTIR

  • Hands-on experience in handling HPLC, GPC, FTIR, Gel Dock, Elisa reader

Relevant Skills

  • Expert Level : ChemDraw, Hyperchem, Origin, Sigmaplot, SPSS, MS-Office

  • Beginner Level: Insight2, Matlab

Research Experience

Doctoral Research, IIT Madras, Jan, 2006 – Dec, 2010.

“Interaction of Natural phytochemicals with Commercial Oral Hypoglycemic Drugs”

Antioxidant studies:

  • Conducted the antioxidant assays for the synthetic compounds as well as natural products,

  • Studied the QSAR studies for both the group of compounds to know the parameter involved in the antioxidant properties.

Cell culture studies:

  • Studied the glucose uptake activity of both natural phytochemicals as well as synthetic compounds with two different cell lines (L6 myotubes and 3T3-L1 adipocytes),

  • Studied the effect of all these compounds on the differentiation of both the cell lines,

  • Did effect of all these compounds on the cellular ATP concentration with HPLC method,

  • Studied the effect of all these compounds on the expression of genes which is involved in glucose uptake by the cells and some down regulators of insulin pathway.

Animal studies:

  • Studied the interaction of phytochemicals with commercial oral antidiabetic drugs on the plasma glucose level, lipid profile, antioxidants status in the animal model (rat),

  • Evaluate the modulation in the insulin cascade at gene level,

  • Anatomical studies of various organs and the beta cell regenerations.

Masters Research, Department of Biochemistry, Patna University Patna

Have done all the biochemical analysis for carbohydrates, lipids and protein for both qualitative as well as quantitative, Microbiological and biochemical tests for the identification of unknown isolated bacterial strain.