Discovering the complexity in intercellular communication

To advance EV analysis through the novel molecular technology at the single EV resolution in vitro and in vivo biological fluids by cutting-edge nano-flow cytometry and super-resolution microscopy

Oncogenic EGFRvIII affecting the EV subtype transition in glioma cells. (A) EGFRvIII-overexpressed glioma U373 (U373vIII) shows the aggressive cellular phenotype with invadopoda structure in which mesenchymal maker BSG and CD44 are highly expressed and colocalized. (B) Nano-flow cytometry shows the increased BSG/CD44 double positive EV subpopulation in U373vIII EVs (Choi et al., Mol Cell Proteomics 2018)

Distinctive CD63- and CD81-positive EV. (A) Structural illuminated microscope (SIM) image shows the anti-CD63 (red) completely colocalize with CD63/GFP protein (green) in A431-CD63/GFP EVs. (B) However, anti-CD81 (red) does not colocalize with CD63/GFP protein (green), implying their distinctive subpopulation in A431-CD63/GFP EVs. (Choi et al., ACS Nano 2019)

2. To understand the landscape of extracellular EV uptake and their intracellular route from the plasma membrane to lysosome for their functionality in cancer.

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