Lab Techniques


Members of the Do Monte lab have the opportunity to learn a wide variety of procedures and techniques in rats, including:

- Optogenetic targeting of projection-specific neuronal populations

- Single-unit electrophysiology in combination with optogenetics to record from identified neuronal populations (photo-tagging)  

- Calcium imaging to measure neuronal network dynamics in freely-behaving animals

- Fiberphotometry and fluorescent biosensors to quantify neurotransmitter release in vivo 

- Immunohistochemical approaches to identify the upregulation/downregulation of specific brain proteins within projection-specific neuronal populations

- Behavioral assays to study the formation and retrieval of aversive and rewarding memories



In addition, students and postdocs have the chance to participate in bi-weekly journal club discussions and develop their skills through oral presentations during our weekly lab meetings. They are also encouraged to present their research results during scientific conferences in the field. Through additional one-on-one weekly meetings with the PI, students are mentored to design hypotheses, develop critical skills, and communicate clearly and effectively through speaking and writing. These individual meetings also allow them to receive constructive feedback according to their individual expertise and abilities. This student-centered training in Do Monte Lab is critical for the rapid development of students, and for the success of the lab as well.