Electromagnetic and UV spectrums
The UV spectrum is the high energy neighbor of visible light. While we cannot see it, the sun produces the full UV spectrum and UV-A/B (280-400 nm) passes through the atmosphere, making its effect known on the surface. If you ever had a sunburn than you have felt the effects of UV-B radiation. The burns themselves can be anything from an annoyance to painful, but overtime the repeated damage to the DNA in your skins cells can lead cancer. It is this same mechanism that makes UV-B light a cancer risk that makes UV-C light an effective germicide.
UV-C and high energy UV-B can interact directly with DNA, producing dimers and other photochemical products that destroy DNA integrity. This process can lead to rapid cell death after sufficient exposure. The effect is most pronounced in UV-C light and can be demonstrated with germicidal effectiveness plots. The one on the right, for E. Coli, shows that UV-C spectrum (200-280nm) is on the whole germicidal. Germicidal effectiveness begins rapidly cutting off in the UV-B spectrum (280-315 nm). The bar at 254 nm represents the emission of low-pressure mercury lamps which have become the standard for germicidal lamps due to their position in the UV-C spectrum.
Germicidal Effectiveness of Light against E. Coli
Fraudulent UV-C Lamps Available on Amazon
Following the shortages seen in the early stages of the COVID-19 pandemic has been a flood of counterfeit and fraudulent products. UV-C lamps have been a target of this fraud, particularly as demand has increased amid the pandemic. These lamps shown on the left, available now on amazon, claim to be UV-C LEDs and almost certainly are not (based on price, reviews testing the lamps, and that it would appear UV-C would be blocked by the plastic bulb material). Because of this rampant fraud, we cannot trust that a lamp we purchase will emit in the UV-C spectrum as advertised. It is critical to the project that we can verify the lamp, and therefore our box, can do what is advertised.
UV-C Meters available on Amazon (pricey)
UV-C sensors available for purchase on amazon are pricey to say the least. This excludes them as a viable option as a means to test our lamp. Instead, we will have to design our own UV-C sensor.
GUVA-S12SD Photodiode Responsivity Curve
All three of these breakout boards are designed around the GUVA-S12SD gallium nitride photodiode. The response curve of this photodiode is shown on the left. We can see that it is blind to visible light and responsive in the UV-A/B and part of the UV-C range. Critically for our application, it is responsive to 254 nm light, which our lamp advertises to be. Any sensor board built around this diode should be responsive to a UV-C light, as the three shown above are. However, upon further examination it is clear that the Proto and Waveshare boards are insufficient for our application. All three of these boards contain op-amps designed to boost the weak currents coming from the photodiode. The Proto and Waveshare use two to boost the response from the relatively weak UV-B intensities from the sun. Our UV lamps will produce UV light intensities far greater than that of the sun, and so these boards will be maxed out at best, damaged at worst.
Transmission Curve for SCHOTT D263 Borosilicate Glass
So we have identified a sensor, the GUVA-S12SD photodiode on the adafruit breakout board, that can detect and respond to UV-C light. However their is a critical problem, the sensor is also responsive to UV-A/B, not UV-C exclusively. This means the sensor board alone will not be able to tell us whether our lamp is UV-C, the key property of a germicidal lamp. The solution to this problem is inspired from the lamp itself. UV-C lamps must use special glass for the bulbs as standard soda-lime glass will block UV-C. Most lamps are built using titanium doped quartz or other similar materials which allow for the full UV spectrum to transmit. If we can find a glass that allows UV-A/B to transmit, but blocks UV-C we can use this to select UV-C and measure its intensity. A long search finally turned up a candidate. SCHOTT glass manufactures produce a high quality borosilicate glass and conveniently cut it into cheap and reasonably sized microscope cover slides. The transmission curve for this glass shown on the left cuts out right at the beginning of the UV-C spectrum. The price, size, and optical properties make this the perfect glass for use in our sensor design.
When the sensor is exposed directly to the light from our lamp we see a response from the output pins on the voltmeter. This tells us that the GUVA photodiode is being activated by this light, in turn telling us our light outputs in the UV spectrum.
Here, we have covered the sensor with the SCHOTT optical glass. We can see that the output voltage is reduced dramatically. This tells us the vast majority of the lights output is being blocked by the glass. From this we can infer that the light is indeed UV-C.
Survival rate of SARS-COV-2 virus exposed to far UV-C (222 nm)
As we can see from the plot on the left small doses of UV-C, on the order of a mJ/cm^2, are needed to eliminate the virus when directly exposed. Combined with information from both the adafruit and GUVA-S12SD datasheets, we can use our light measurements to estimate a lamp intensity of ~8 mW/cm^2 at 10 cm below the center of the lamp. This means our lamp should be able to effectively eliminate the virus in under a second. However, masks provide extra cover for the virus, and studies done on disinfecting masks from influenza virus have shown that roughly 1 J/cm^2 is needed to disinfect a mask. For our light, that should take roughly 125 seconds, but we will set our disinfection cycle to 300 seconds, covering for errors in our calculation, lamp degradation, and the likely reduced intensities the ends of masks will experience.
This procedure gives us a relatively low cost and effective way to test if a lamp is germicidal. This measurement will be crucial in future design considerations. Tests and measurements were taken when the sensors were first received were used in this calculation. When implemented in the box these sensors proved tricky to use for any reliable intensity calculations. For one, they were somewhat weakly responsive to the intensities of the UV-C lamp, and so could not be used for high resolution measurements on the arduino and were also susceptible to noise. Moreover, they were sensitive to physical disruption (a new soldering job may be in order). That being said, the sensors were still effective at detecting the presence of UV-C light in the box and make an effective check that the light is on.
In a future project, a custom pcb can be made using the GUVA photodiode that is better fitted to the output intensities of typical consumer grade germicidal lamps. These could be used to at a minimum reliably check if a lamp is UV-C and at best give reliable if somewhat low precision UV-C intensity measurements.